Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Fas Ligand/TNFSF6 Quantikine ELISA Kit Summary

• Background: The Quantikine^® Human Fas Ligand/TNFSF6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves tha...t were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Fas Ligand.
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• Specificity: Natural and recombinant human Fas Ligand.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

• apoptosis (APO-1) antigen ligand 1
• Apoptosis antigen ligand
• APT1LG1CD95L
• APTL
• CD178 antigen
• CD178
• CD95L
• CD95-L
• Fas antigen ligand
• Fas ligand (TNF superfamily, member 6)
• Fas Ligand
• FASLCD95 ligand
• FASLG
• TNFSF6
• TNFSF6FasL
• tumor necrosis factor (ligand) superfamily, member 6
• tumor necrosis factor ligand superfamily member 6

Background

Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Fas Ligand/TNFSF6 (DFL00B)(29)

Publications using DFL00B

• FS Thaler, B Bangol, M Biljecki, J Havla, AM Schumacher, T Kümpfel Possible link of genetic variants to autoimmunity in GAD-antibody-associated neurological disorders J. Neurol. Sci., 2020-04-29;413(0):116860. 2020-04-29 [PMID: 32388243] (Human)
• A Masuda, Y Isobe, K Sugimoto, M Yoshimori, A Arai, N Komatsu Efficient recruitment of c-FLIPL to the death-inducing signaling complex leads to Fas resistance in natural killer-cell lymphoma Cancer Sci., 2020-01-31;111(3):807-816. 2020-01-31 [PMID: 31908105] (Human)
• R Grzanka, A Damasiewic, A Kasperska- Tumor necrosis factor-alpha and Fas/Fas ligand signaling pathways in chronic spontaneous urticaria Allergy Asthma Clin Immunol, 2019-03-14;15(0):15. 2019-03-14 [PMID: 30911316] (Human)
• Y Matsukiyo, H Nagai, T Matsui, Y Igarashi Host Immunological Effects of Partial Splenic Embolization in Patients with Liver Cirrhosis J Immunol Res, 2018-07-15;2018(0):1746391. 2018-07-15 [PMID: 30116748] (Human)
• A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu Evaluation of soluble concentration Fas and Fas ligand in maternal and cord blood 3rd trimester of pregnancy Ginekol. Pol., 2018-01-01;89(3):142-146. 2018-01-01 [PMID: 29664549] (Human)
• Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis Sci Rep, 2016-11-18;6(0):36862. 2016-11-18 [PMID: 27857173] (Human)
• Katz , Gil, Krummey , Scott M, Larsen , Sasha E, Stinson , Jeffrey, Snow , Andrew L SAP facilitates recruitment and activation of LCK at NTB-A receptors during restimulation-induced cell death. J Immunol, 2014-03-31;192(9):4202-9. 2014-03-31 [PMID: 24688028] (Human)
• Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation. J Biol Chem, 2014-03-13;289(16):11522-35. 2014-03-13 [PMID: 24627480] (Mouse)
• Price S, Shaw P, Seitz A, Joshi G, Davis J, Niemela J, Perkins K, Hornung R, Folio L, Rosenberg P, Puck J, Hsu A, Lo B, Pittaluga S, Jaffe E, Fleisher T, Rao V, Lenardo M Natural history of autoimmune lymphoproliferative syndrome associated with FAS gene mutations. Blood, 2014-01-07;123(13):1989-99. 2014-01-07 [PMID: 24398331] (Human)
• Beynon V, Quintana FJ, Weiner HL Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling. PLoS ONE, 2012-06-29;7(6):e39576. 2012-06-29 [PMID: 22768094] (Human)
• Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients. Mucosal Immunol, 2011-12-21;5(2):140-9. 2011-12-21 [PMID: 22189956] (Human)
• Tang TT, Zhu ZF, Wang J, Zhang WC, Tu X, Xiao H, Du XL, Xia JH, Dong NG, Su W, Xia N, Yan XX, Nie SF, Liu J, Zhou SF, Yao R, Xie JJ, Jevallee H, Wang X, Liao MY, Shi GP, Fu M, Liao YH, Cheng X Impaired thymic export and apoptosis contribute to regulatory T-cell defects in patients with chronic heart failure. PLoS ONE, 2011-09-15;6(9):e24272. 2011-09-15 [PMID: 21935395] (Human)
• Dowdell KC, Niemela JE, Price S, Davis J, Hornung RL, Oliveira JB, Puck JM, Jaffe ES, Pittaluga S, Cohen JI, Fleisher TA, Rao VK Somatic FAS mutations are common in patients with genetically undefined autoimmune lymphoproliferative syndrome. Blood, 2010-04-01;115(25):5164-9. 2010-04-01 [PMID: 20360470] (Human)
• Deftereos S, Giannopoulos G, Kossyvakis C, Kaoukis A, Raisakis K, Driva M, Panagopoulou V, Lappos S, Rentoukas I, Pyrgakis V, Alpert MA Effect of quinapril on in-stent restenosis and relation to plasma apoptosis signaling molecules. Am. J. Cardiol., 2009-11-14;105(1):54-8. 2009-11-14 [PMID: 20102890] (Human)
• Zhao WS, Xu L, Wang LF, Zhang L, Zhang ZY, Liu Y, Liu XL, Yang XC, Cui L, Zhang L A 60-s postconditioning protocol by percutaneous coronary intervention inhibits myocardial apoptosis in patients with acute myocardial infarction. Apoptosis, 2009-10-01;14(10):1204-11. 2009-10-01 [PMID: 19693675] (Human)
• Liao WT, Yu CL, Lan CC, Lee CH, Chang CH, Chang LW, You HL, Yu HS Differential effects of arsenic on cutaneous and systemic immunity: focusing on CD4+ cell apoptosis in patients with arsenic-induced Bowen's disease. Carcinogenesis, 2009-04-17;30(6):1064-72. 2009-04-17 [PMID: 19376847] (Human)
• Tohyama M, Shirakata Y, Sayama K, Hashimoto K The influence of hepatic damage on serum soluble Fas ligand levels of patients with drug rashes. J. Allergy Clin. Immunol., 2009-04-01;123(4):971-2; author. 2009-04-01 [PMID: 19348934] (Human)
• Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus. J. Exp. Med., 2009-03-16;206(4):793-805. 2009-03-16 [PMID: 19289577] (Human)
• Chung WH, Hung SI, Yang JY, Su SC, Huang SP, Wei CY, Chin SW, Chiou CC, Chu SC, Ho HC, Yang CH, Lu CF, Wu JY, Liao YD, Chen YT Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis. Nat. Med., 2008-11-23;14(12):1343-50. 2008-11-23 [PMID: 19029983] (Human)
• Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 2008-05-27;36(8):1035-46. 2008-05-27 [PMID: 18504067] (Human)
• Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure. Atherosclerosis, 2007-11-07;199(1):215-21. 2007-11-07 [PMID: 17991471] (Human)
• Zhang B, Sun T, Xue L, Han X, Zhang B, Lu N, Shi Y, Tan W, Zhou Y, Zhao D, Zhang X, Guo Y, Lin D Functional polymorphisms in FAS and FASL contribute to increased apoptosis of tumor infiltration lymphocytes and risk of breast cancer. Carcinogenesis, 2006-12-20;28(5):1067-73. 2006-12-20 [PMID: 17183065] (Human)
• Harris LK, Keogh RJ, Wareing M, Baker PN, Cartwright JE, Aplin JD, Whitley GS Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism. Am. J. Pathol., 2006-11-01;169(5):1863-74. 2006-11-01 [PMID: 17071607] (Human)
• Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock. Am. J. Respir. Crit. Care Med., 2006-03-30;174(1):51-7. 2006-03-30 [PMID: 16574939] (Human)
• Pignatelli P, Sanguigni V, Paola SG, Lo Coco E, Lenti L, Violi F Vitamin C inhibits platelet expression of CD40 ligand. Free Radic. Biol. Med., 2005-03-23;38(12):1662-6. 2005-03-23 [PMID: 15917194] (Human)
• Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy. Eur J Cardiovasc Prev Rehabil, 2005-04-01;12(2):164-8. 2005-04-01 [PMID: 15785303] (Human)
• Zhang L, Li YH, Zhang HY, Chen ML, Gao MM, Hu AH, Yang HS High-dose glucose-insulin-potassium treatment reduces myocardial apoptosis in patients with acute myocardial infarction. Eur. J. Clin. Invest., 2005-03-01;35(3):164-70. 2005-03-01 [PMID: 15733070] (Human)
• Alter P, Maisch B Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors. Z Kardiol, 2004-07-01;93(7):524-32. 2004-07-01 [PMID: 15243763] (Human)
• D'Archivio M Wheat gliadin induces apoptosis of intestinal cells via an autocrine mechanism involving Fas-Fas ligand pathway. FEBS Lett., 2003-04-10;540(1):117-24. 2003-04-10 [PMID: 12681494] (Human)