RPL22 Antibody

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - (3.8 ug/ml) staining of paraffin embedded Human Cortex Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RPL22 Antibody Summary

• Immunogen: Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RPL22
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave bands at approx. 17-18 kDa band and 25 kDa band in human liver lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8 kDa band according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human brain (cortex) shows cytoplasm staining in neuronal bodies.
• Positive Control:

  RPL22 Lysate (NBP2-65891)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RPL22 Antibody

• EAPHBP15
• EBER-associated protein
• Epstein-Barr virus small RNA-associated protein
• Epstein-Barr-encoded RNA-associated protein
• HBP15/L22
• heparin-binding protein 15
• Heparin-binding protein HBp15
• ribosomal protein L22,60S ribosomal protein L22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPL22 Antibody (NBP1-06069)(4)

Publications using NBP1-06069

• Li L, Wang M, Wang M et al. A long non-coding RNA interacts with Gfra1 and maintains survival of mouse spermatogonial stem cells Cell Death Dis Mar 11 2016 [PMID: 26962690] (WB, Mouse)
• Lin SJ, Chang KP, Hsu CW et al. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics. 2013 Sep 27 [PMID: 24080422] (WB, IHC-P, Human)
• Jin J, Jing W, Lei X-X et al. Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes. Nucleic Acids Res. 2011 [PMID: 21247876]
• Anderson SJ, Lauritsen JP, Hartman MG et al. Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint. Immunity 2007 Jun [PMID: 17555992]

Bioinformatics

• Gene Symbol: RPL22
• Entrez:
  • 6146(Human)
  • 19934(Mouse)
  • 81768(Rat)
• Uniprot:
  • P35268(Human)