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RPL22 Antibody

 (6 Publications)
 (1 Review)
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Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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RPL22 Antibody Summary

Immunogen
Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPL22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 3 - 6 ug/mL
  • Immunohistochemistry-Paraffin 3 - 6 ug/mL
  • Peptide ELISA Detection limit 1:16000
Control
RPL22 Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06069 in the following applications:
Publications
Read Publications using
NBP1-06069 in the following applications:

  • IHC-P
    1 publication
  • WB
    4 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26962690).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPL22 Antibody

  • EAPHBP15
  • EBER-associated protein
  • Epstein-Barr virus small RNA-associated protein
  • Epstein-Barr-encoded RNA-associated protein
  • HBP15/L22
  • heparin-binding protein 15
  • Heparin-binding protein HBp15
  • ribosomal protein L22,60S ribosomal protein L22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: RPL4 Antibody (4A3) [H00006124-M01] - RPL4 monoclonal antibody (M01), clone 4A3 Analysis of RPL4 expression in K-562.
Immunocytochemistry/Immunofluorescence: RPL4 Antibody (4A3) [H00006124-M01] - Analysis of monoclonal antibody to RPL4 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: RPL4 Antibody (4A3) [H00006124-M01] - RPL4 monoclonal antibody (M01), clone 4A3. Analysis of RPL4 expression in PC-12.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
FAM60A Antibody
H00058516-B02P
Western Blot: FAM60A Antibody [H00058516-B02P] - Analysis of FAM60A expression in transfected 293T cell line by FAM60A polyclonal antibody. Lane 1: FAM60A transfected lysate(24.31 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: FAM60A Antibody [H00058516-B02P] - Analysis of purified antibody to FAM60A on HeLa cell. (antibody concentration 30 ug/ml)
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
RUNX1/CBFA2
Antibody
NBP1-89105
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining in human breast and liver tissues using NBP1-89105 antibody. Corresponding RUNX1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining of human breast shows moderate to strong nuclear positivity in glandular cells.
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
60S ribosomal
protein L23 Antibody
NBP1-87847
Western Blot: 60S ribosomal protein L23 Antibody [NBP1-87847] - Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: 60S ribosomal protein L23 Antibody [NBP1-87847] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: 60S ribosomal protein L23 Antibody [NBP1-87847] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
EVI-1 Antibody
28100002
ELISA: EVI-1 Antibody [28100002]
(1 Review)
(3 Publications)
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
Ribosomal Protein
L17 Antibody (702142)
MAB7158
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Ribosomal Protein L17 Monoclonal Antibody (Catalog # MAB7158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein L17 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ribosomal Protein L17 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Ribosomal Protein L17 Monoclonal Antibody (Catalog # MAB7158) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of acinar cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples.
Species: Hu, Mu
Applications: WB, IHC
PPFIBP1 Antibody
NBP1-83192
Western Blot: PPFIBP1 Antibody [NBP1-83192] - Analysis in human cell lines Caco-2 and HEK293 using Anti-PPFIBP1 antibody. Corresponding PPFIBP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Immunocytochemistry/Immunofluorescence: PPFIBP1 Antibody [NBP1-83192] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
Immunohistochemistry-Paraffin: PPFIBP1 Antibody [NBP1-83192] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells and adnexal cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
RPL3 Antibody
NBP2-20214
Western Blot: RPL3 Antibody [NBP2-20214] - Sample (30 ug of whole cell lysate) A: JurKat B: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: RPL3 Antibody [NBP2-20214] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: RPL3 Antibody [NBP2-20214] - Paraffin-embedded mouse intestine diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
ZACN Antibody
NBP1-80104
Western Blot: ZACN Antibody [NBP1-80104] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.
Species: Hu
Applications: WB
RPS3 Antibody
NBP1-33691
Western Blot: RPS3 Antibody [NBP1-33691] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS3 antibody diluted at a dilution of 1:500.
Immunocytochemistry/Immunofluorescence: RPS3 Antibody [NBP1-33691] - Analysis of methanol-fixed HeLa, using RPS3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: RPS3 Antibody [NBP1-33691] - Mouse intestine diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
RPS19 Antibody
(JU33-43)
NBP2-75649
Western Blot: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of RPS19 on Hela cell using anti-RPS19 antibody at 1/500 dilution.Positive control:Lane 1: CRC Lane 2: MCF-7
Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded rat brain tissue using anti-RPS19 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded human colon cancer tissue using anti-RPS19 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
LASS2 Antibody
(1A6)
H00029956-M01A
Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.
Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - S2 monoclonal antibody (M01), clone 1A6 Western Blot analysis of LASS2 expression in HepG2.
Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - S2 monoclonal antibody (M01), clone 1A6. Western Blot analysis of LASS2 expression in NIH/3T3.
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: WB, ELISA, IHC
RPL29 Antibody
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Western Blot: RPL29 Antibody [H00006159-B02P] - Analysis of RPL29 expression in human pancreas.
Immunocytochemistry/Immunofluorescence: RPL29 Antibody [H00006159-B02P] - Analysis of purified antibody to RPL29 on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: RPL29 Antibody [H00006159-B02P] - Analysis of purified antibody to RPL29 on formalin-fixed paraffin-embedded human salivary gland. (antibody concentration 3 ug/ml)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
TPGS2 Antibody
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Western Blot: TPGS2 Antibody [NBP2-20687] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: TPGS2 Antibody [NBP2-20687] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, ICC/IF
PDLIM7 Antibody
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Western Blot: PDLIM7 Antibody [NBP1-84841] - Analysis using Anti-PDLIM7 antibody NBP1-84841 (A) shows similar pattern to independent antibody NBP2-58734 (B).
Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody [NBP1-84841] - Staining of human cell line U-251 MG shows localization to actin filaments and focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDLIM7 Antibody [NBP1-84841] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
(2 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
RPL14 Antibody
NBP2-97838
Western Blot: RPL14 Antibody [NBP2-97838] - Anti-RPL14 rabbit polyclonal antibody at 1:2000 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 26 kDa
Immunohistochemistry-Paraffin: RPL14 Antibody [NBP2-97838] - Immunochemical staining of human RPL14 in human rectum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: RPL14 Antibody [NBP2-97838] - Immunochemical staining of human RPL14 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Species: Hu
Applications: WB, IHC, IHC-P, IP
RPS10 Antibody
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Western Blot: RPS10 Antibody [NBP1-31357] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: RPS10 Antibody [NBP1-31357] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RPS10 protein stained by RPS10 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: RPS10 Antibody [NBP1-31357] - Paraffin-embedded mouse colon diluted at 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Ribosomal Protein
L24 Antibody
NBP2-93788
Western Blot: Ribosomal Protein L24 Antibody [NBP2-93788] - Analysis of extracts of various cell lines, using Ribosomal Protein L24 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/Immunofluorescence: Ribosomal Protein L24 Antibody [NBP2-93788] - Analysis of L929 cells using Ribosomal Protein L24. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Ribosomal Protein L24 Antibody [NBP2-93788] - Paraffin-embedded human thyroid cancer using Ribosomal Protein L24.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
RPL18 Antibody
NBP2-13251
Western Blot: RPL18 Antibody [NBP2-13251] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: RPL18 Antibody [NBP2-13251] - Staining of human cell line HEK 293 shows localization to nucleoli, cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RPL18 Antibody [NBP2-13251] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
TEX10
Overexpression Lysate
NBP2-06930
Western Blot: TEX10 Overexpression Lysate (Adult Normal) [NBP2-06930] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TEX10.
Species: Hu

Publications for RPL22 Antibody (NBP1-06069)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(4)
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(2)
Mouse
(2)
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Review for RPL22 Antibody (NBP1-06069) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-06069:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot RPL22 NBP1-06069
Enlarge 		4
reviewed by:
Anonymous
 WB Human 11/02/2020
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Summary

ApplicationWestern Blot
Sample TestedHEK293 cell lysate
SpeciesHuman
LotP1 E071019

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FAQs for RPL22 Antibody (NBP1-06069) (0)

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Control Lysate(s)

RPL22 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Array NBP1-06069

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Diseases for RPL22 Antibody (NBP1-06069)

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Western Blot RPL22 NBP1-06069
Enlarge
 Anonymous
11/02/2020
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol RPL22
Entrez
Uniprot