RPL22 Antibody

Images

 
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - (3.8 ug/ml) staining of paraffin embedded Human Cortex Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

RPL22 Antibody Summary

Immunogen
Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPL22
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA detection limit 1:16000
Application Notes
WB: Preliminary experiments gave bands at approx. 17-18 kDa band and 25 kDa band in human liver lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8 kDa band according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human brain (cortex) shows cytoplasm staining in neuronal bodies.
Positive Control
RPL22 Lysate (NBP2-65891)
Publications
Read Publications using
NBP1-06069 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPL22 Antibody

  • EAPHBP15
  • EBER-associated protein
  • Epstein-Barr virus small RNA-associated protein
  • Epstein-Barr-encoded RNA-associated protein
  • HBP15/L22
  • heparin-binding protein 15
  • Heparin-binding protein HBp15
  • ribosomal protein L22,60S ribosomal protein L22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RPL22 Antibody (NBP1-06069)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RPL22 Antibody (NBP1-06069) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPL22
Entrez
Uniprot