RPL22 Antibody

Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for 1 h, and probing for hnRNP H/F or RPL22 (negative control), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. Shown is a representative result from n = 2 independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-16168-x) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RPL22 Antibody Summary

• Immunogen: Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RPL22
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/mL
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA detection limit 1:16000
  • Western Blot 1:100-1:2000

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26962690).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RPL22 Antibody

• EAPHBP15
• EBER-associated protein
• Epstein-Barr virus small RNA-associated protein
• Epstein-Barr-encoded RNA-associated protein
• HBP15/L22
• heparin-binding protein 15
• Heparin-binding protein HBp15
• ribosomal protein L22,60S ribosomal protein L22

Background

RPL22, also known as 60S ribosomal protein L22, is a 15kDa 128 amino acid protein. The primary function of RPL22 is to encode a cytoplasmic ribosomal protein. Current research is being conducted on RPL22 in relation to hepatitis, carcinoma, malaria, leukemia, hepatitis c, pneumonia and lung carcinoma. RPL22 has been linked to the virulence, methylation, contact inhibition, pathogenesis and cell growth pathways where it interacts with SHC1, UBA5, CSE1L, PRKAG1 and MAP3K14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RPL22 Antibody (NBP1-06069)(8)

Publications using NBP1-06069

• Luan y, Tang N, yang J et al. Deficiency of ribosomal proteins reshapes the transcriptional and translational landscape in human cells Nucleic acids research 2022-02-08 [PMID: 35137207] (WB, Human)
• Bogdanov V, Soltisz AM, Moise N Et al. Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes Basic research in cardiology 2021-10-28 [PMID: 34713358] (IF/IHC, ICC/IF, Mouse)
• Herviou P, Le Bras M, Dumas L et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma Nat Commun 2020-05-27 [PMID: 32461552] (WB, Human)
• Fu K, Tian S, Tan H Biological and RNA regulatory function of MOV10 in mammalian germ cells BMC Biol. 2019-05-14 [PMID: 31088452] (WB, Mouse)
• Li L, Wang M, Wang M et al. A long non-coding RNA interacts with Gfra1 and maintains survival of mouse spermatogonial stem cells Cell Death Dis 2016-03-11 [PMID: 26962690] (WB, Mouse)
• Lin SJ, Chang KP, Hsu CW et al. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics 2013-09-27 [PMID: 24080422] (WB, IHC-P, Human)
• Jin J, Jing W, Lei X-X et al. Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes. Nucleic Acids Res. 2011-01-01 [PMID: 21247876]
• Anderson SJ, Lauritsen JP, Hartman MG et al. Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint. Immunity 2007-06-01 [PMID: 17555992]

Review for RPL22 Antibody (NBP1-06069) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Weijin Xu WB Human 11/02/2020

Summary

• Application: Western Blot
• Sample Tested: HEK293 cell lysate
• Species: Human
• Lot: P1 E071019

Bioinformatics

• Gene Symbol: RPL22
• Entrez:
  • 6146(Human)
  • 19934(Mouse)
  • 81768(Rat)
• Uniprot:
  • P35268(Human)