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Home » RPL22 » RPL22 Antibodies » RPL22 Antibody

RPL22 Antibody

 (8 Publications)
 (1 Review)
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Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for 1 h, and probing for hnRNP H/F or RPL22 (negative control), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. Shown is a representative result from n = 2 independent experiments. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41467-020-16168-x) licensed under a CC-BY licence.
Western Blot: RPL22 Antibody [NBP1-06069] - hnRNP H/F subcellular localization and association with polysomal fractions. Polysome profile of U251 cells untreated (NT) or treated with 20 uM carboxypyridostatin (cPDS) for ...read more
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Cortex. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Thyroid. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RPL22 Antibody [NBP1-06069] - Staining of Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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RPL22 Antibody Summary

Immunogen
Peptide with sequence C-SKESYELRYFQINQ corresponding to C-Terminus according to NP_000974.1.
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPL22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/mL
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA detection limit 1:16000
  • Western Blot 1:100-1:2000
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06069 in the following applications:
Publications
Read Publications using
NBP1-06069 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26962690).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RPL22 Antibody

  • EAPHBP15
  • EBER-associated protein
  • Epstein-Barr virus small RNA-associated protein
  • Epstein-Barr-encoded RNA-associated protein
  • HBP15/L22
  • heparin-binding protein 15
  • Heparin-binding protein HBp15
  • ribosomal protein L22,60S ribosomal protein L22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00006124-M01
Western Blot: RPL4 Antibody (4A3) [H00006124-M01] - RPL4 monoclonal antibody (M01), clone 4A3 Analysis of RPL4 expression in K-562.
Immunocytochemistry/Immunofluorescence: RPL4 Antibody (4A3) [H00006124-M01] - Analysis of monoclonal antibody to RPL4 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: RPL4 Antibody (4A3) [H00006124-M01] - Detection limit for recombinant GST tagged RPL4 is approximately 0.3ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
FAM60A Antibody
H00058516-B02P
Western Blot: FAM60A Antibody [H00058516-B02P] - Analysis of FAM60A expression in transfected 293T cell line by FAM60A polyclonal antibody. Lane 1: FAM60A transfected lysate(24.31 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: FAM60A Antibody [H00058516-B02P] - Analysis of purified antibody to FAM60A on HeLa cell. (antibody concentration 30 ug/ml)
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
RUNX1/CBFA2
Antibody
NBP1-89105
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining human cell line A-431 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining of human breast shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody [NBP1-89105] - Staining of human Fallopian tube shows weak to moderate nuclear positivity in glandular cells.
(1 Review)
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
60S ribosomal
protein L23 Antibody
NBP1-87847
Immunocytochemistry/Immunofluorescence: 60S ribosomal protein L23 Antibody [NBP1-87847] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Western Blot: 60S ribosomal protein L23 Antibody [NBP1-87847] - Analysis in human cell line THP-1.
Immunohistochemistry-Paraffin: 60S ribosomal protein L23 Antibody [NBP1-87847] - Staining of human pancreas shows strong cytoplasmic positivity in excorine glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
EVI-1 Antibody
NBP2-48848
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human kidney shows strong nuclear positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: EVI-1 Antibody [NBP2-48848] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Ribosomal Protein
L17 Antibody (702142)
MAB7158
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Ribosomal Protein L17 Monoclonal Antibody (Catalog # MAB7158) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein L17 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Ribosomal Protein L17 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Ribosomal Protein L17 Monoclonal Antibody (Catalog # MAB7158) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of acinar cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples.
Species: Hu, Mu
Applications: IHC, WB
PPFIBP1 Antibody
NBP1-83192
Immunohistochemistry-Paraffin: PPFIBP1 Antibody [NBP1-83192] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells and adnexal cells.
Immunocytochemistry/Immunofluorescence: PPFIBP1 Antibody [NBP1-83192] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
Western Blot: PPFIBP1 Antibody [NBP1-83192] - Analysis in human cell lines Caco-2 and HEK293 using Anti-PPFIBP1 antibody. Corresponding PPFIBP1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
RPL3 Antibody
NBP2-20214
Western Blot: RPL3 Antibody [NBP2-20214] - Sample (30 ug of whole cell lysate) A: JurKat B: Raji 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: RPL3 Antibody [NBP2-20214] - 4T1 xenograft, using RPL3 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunocytochemistry/Immunofluorescence: RPL3 Antibody [NBP2-20214] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
ZACN Antibody
NBP1-80104
Western Blot: ZACN Antibody [NBP1-80104] - NCI-H226 cell lysate, concentration 0.2-1 ug/ml.
Species: Hu
Applications: WB
RPS3 Antibody
(SA46-08)
NBP2-67301
Immunocytochemistry/Immunofluorescence: RPS3 Antibody (SA46-08) [NBP2-67301] - Staining RPS3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RPS3 Antibody (SA46-08) [NBP2-67301] - Analysis of paraffin-embedded human kidney tissue using anti-RPS3 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RPS3 Antibody (SA46-08) [NBP2-67301] - Analysis of paraffin-embedded mouse lung tissue using anti-RPS3 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
RPS19 Antibody
(JU33-43)
NBP2-75649
Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded human colon cancer tissue using anti-RPS19 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded mouse colon tissue using anti-RPS19 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RPS19 Antibody (JU33-43) [NBP2-75649] - Analysis of paraffin-embedded mouse brain tissue using anti-RPS19 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
LASS2 Antibody
(1A6)
H00029956-M01A
Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - S2 monoclonal antibody (M01), clone 1A6 Western Blot analysis of LASS2 expression in HepG2.
Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - S2 monoclonal antibody (M01), clone 1A6. Western Blot analysis of LASS2 expression in NIH/3T3.
Western Blot: LASS2 Antibody (1A6) [H00029956-M01A] - Human neuroblastoma cells untreated or treated with various drugs. Image from verified customer review.
(1 Review)
(7 Publications)
Species: Hu, Mu
Applications: ELISA, IHC, WB
RPL29 Antibody
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Western Blot: RPL29 Antibody [NBP3-12600] - Lane A: PC-3 Whole Cell LysateLane B: U87-MG Whole Cell LysateLane C: SW480 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:18 kDaObserved band size:22 kDa
Immunohistochemistry-Paraffin: RPL29 Antibody [NBP3-12600] - Staining of human RPL29 in human brain with rabbit polyclonal antibody at 1:10000 dilution.
Immunohistochemistry-Paraffin: RPL29 Antibody [NBP3-12600] - Staining of human RPL29 in human liver with rabbit polyclonal antibody at 1:10000 dilution.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
TPGS2 Antibody
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Western Blot: TPGS2 Antibody [NBP2-20687] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: TPGS2 Antibody [NBP2-20687] - Analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.
Species: Hu
Applications: ICC/IF, WB
PDLIM7 Antibody
(2D6)
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Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of monoclonal antibody to PDLIM7 on HeLa cell. Antibody concentration 10 ug/ml.
Immunoprecipitation: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 transfected lysate using anti-PDLIM7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PDLIM7 MaxPab rabbit polyclonal antibody.
Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 over-expressed 293 cell line, cotransfected with PDLIM7 Validated Chimera RNAi ( Cat # H00009260-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PDLIM7 monoclonal antibody (M01), clone 2D6 (Cat # H00009260-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
(1 Publication)
Species: Hu, Rt
Applications: ELISA, ICC/IF, IP, KD, S-ELISA, WB
RPL14 Antibody
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Immunohistochemistry-Paraffin: RPL14 Antibody [NBP2-97838] - Immunochemical staining of human RPL14 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: RPL14 Antibody [NBP2-97838] - Immunochemical staining of human RPL14 in human rectum with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: RPL14 Antibody [NBP2-97838] - RPL14 was immunoprecipitated using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 ul anti-RPL14 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RPL14 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 26 kDa
Species: Hu
Applications: IHC, IHC-P, IP, WB
RPS10 Antibody
NBP2-94502
Immunocytochemistry/Immunofluorescence: RPS10 Antibody [NBP2-94502] - Analysis of MCF7 cells using RPS10 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPS10 Antibody [NBP2-94502] - Paraffin-embedded mouse brain using RPS10 .
Immunohistochemistry-Paraffin: RPS10 Antibody [NBP2-94502] - Paraffin-embedded rat brain using RPS10 .
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Ribosomal Protein
L24 Antibody
NBP2-93788
Immunocytochemistry/Immunofluorescence: Ribosomal Protein L24 Antibody [NBP2-93788] - Analysis of L929 cells using Ribosomal Protein L24 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Ribosomal Protein L24 Antibody [NBP2-93788] - Paraffin-embedded human colon carcinoma using Ribosomal Protein L24 .
Western Blot: Ribosomal Protein L24 Antibody [NBP2-93788] - Analysis of extracts of various cell lines, using Ribosomal Protein L24 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
RPL18 Antibody
NBP2-13251
Immunohistochemistry-Paraffin: RPL18 Antibody [NBP2-13251] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunocytochemistry/Immunofluorescence: RPL18 Antibody [NBP2-13251] - Staining of human cell line HEK 293 shows localization to nucleoli, cytosol & endoplasmic reticulum. Antibody staining is shown in green.
Western Blot: RPL18 Antibody [NBP2-13251] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
TEX10 Antibody
NBP3-09260
Western Blot: TEX10 Antibody [NBP3-09260] - Western blot analysis of TEX10 in Fetal Brain lysates. Antibody dilution at 1.0ug/ml
Species: Hu
Applications: WB

Publications for RPL22 Antibody (NBP1-06069)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


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ICC/IF
(1)
IHC
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(5)
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(3)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-06069 Applications Species
Luan y, Tang N, yang J et al. Deficiency of ribosomal proteins reshapes the transcriptional and translational landscape in human cells Nucleic acids research Feb 8 2022 [PMID: 35137207] (WB, Human) WB Human
Bogdanov V, Soltisz AM, Moise N Et al. Distributed synthesis of sarcolemmal and sarcoplasmic reticulum membrane proteins in cardiac myocytes Basic research in cardiology Oct 28 2021 [PMID: 34713358] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Herviou P, Le Bras M, Dumas L et al. hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma Nat Commun May 27 2020 [PMID: 32461552] (WB, Human) WB Human
Fu K, Tian S, Tan H Biological and RNA regulatory function of MOV10 in mammalian germ cells BMC Biol. May 14 2019 [PMID: 31088452] (WB, Mouse) WB Mouse
Li L, Wang M, Wang M et al. A long non-coding RNA interacts with Gfra1 and maintains survival of mouse spermatogonial stem cells Cell Death Dis Mar 11 2016 [PMID: 26962690] (WB, Mouse) WB Mouse
Lin SJ, Chang KP, Hsu CW et al. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics. 2013 Sep 27 [PMID: 24080422] (WB, IHC-P, Human) WB, IHC-P Human
Jin J, Jing W, Lei X-X et al. Evidence that Lin28 stimulates translation by recruiting RNA helicase A to polysomes. Nucleic Acids Res. 2011 [PMID: 21247876]
Anderson SJ, Lauritsen JP, Hartman MG et al. Ablation of Ribosomal Protein L22 Selectively Impairs alphabeta T Development by Activation of a p53-Dependent Checkpoint. Immunity 2007 Jun [PMID: 17555992]

Review for RPL22 Antibody (NBP1-06069) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-06069:
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Images Ratings Applications Species Date Details
Western Blot RPL22 NBP1-06069
Enlarge 		4
reviewed by:
Verified Customer
 WB Human 11/02/2020
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Summary

ApplicationWestern Blot
Sample TestedHEK293 cell lysate
SpeciesHuman
LotP1 E071019

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FAQs for RPL22 Antibody (NBP1-06069) (0)

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Secondary Antibodies

 

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Array NBP1-06069

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Diseases for RPL22 Antibody (NBP1-06069)

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Western Blot RPL22 NBP1-06069
Enlarge
 Verified Customer
11/02/2020
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol RPL22
Entrez
Uniprot