PRDM16/MEL1 Antibody

Images

 
Western Blot: PRDM16/MEL1 Antibody [NBP1-77096] - Analysis of PRDM16 in K562 cell lysate with PRDM16 antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: PRDM16/MEL1 Antibody [NBP1-77096] - PRDM16 in mouse brain tissue with PRDM16 antibody at 20 ug/ml.
Immunohistochemistry: PRDM16/MEL1 Antibody [NBP1-77096] - Immunohistochemistry of PRDM16 in mouse brain tissue with PRDM16 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PRDM16/MEL1 Antibody [NBP1-77096] - in Human Brain cells with PRDM16 antibody at 20 ug/mL.
Immunohistochemistry: PRDM16/MEL1 Antibody [NBP1-77096] - PRDM16 in human brain tissue with PRDM16 antibody at 2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PRDM16/MEL1 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120 - 1170 of PRDM16.
Specificity
Recognizes 120, 138 and 140 kDa isoforms of PRDM16
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRDM16
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL.
Theoretical MW
140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PRDM16/MEL1 Peptide (NBP1-77096PEP)
Publications
Read Publication using
NBP1-77096 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PRDM16/MEL1 Antibody

  • MDS1/EVI1-like gene 1
  • MEL1
  • MEL1KIAA1675PFM13MGC166915
  • PFM13
  • PR domain containing 16
  • PR domain zinc finger protein 16
  • PR domain-containing protein 16
  • PRDM16
  • Transcription factor MEL1

Background

PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRDM16/MEL1 Antibody (NBP1-77096)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PRDM16
Entrez
Uniprot