Ribophorin I Antibody (OTI3H3)

Images

 
Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.
Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells transfected with either Ribophorin I Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained ...read more
Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Ribophorin I Antibody (OTI3H3) Summary

Immunogen
Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence -Validated from a verified customer review.
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
66.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-01294 in the following applications:

Publications
Read Publication using
NBP2-01294 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribophorin I Antibody (OTI3H3)

  • DKFZp686B16177
  • dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
  • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
  • EC 2.4.1.119
  • oligosaccharyltransferase 1 homolog
  • OST1
  • ribophorin IRBPH1
  • ribophorin-1
  • RPN-I

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ribophorin I Antibody (NBP2-01294)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Reviews for Ribophorin I Antibody (NBP2-01294) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-01294:
Filter by Applications
WB
(1)
ICC
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot Ribophorin I NBP2-01294
Enlarge
5
reviewed by:
Teddy Chan
WB Human 02/08/2019
View

Summary

ApplicationWestern Blot
Sample TestedBrain Vascular Smooth Muscle Cell lysate
SpeciesHuman
LotA01

Comments

CommentsBetter reactivity when samples are not boiled
Immunocytochemistry Ribophorin I NBP2-01294
Enlarge
4
reviewed by:
Verified Customer
ICC Human 07/01/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cells tumor and non tumor
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ribophorin I Antibody (NBP2-01294) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Teddy Chan
02/08/2019
Application: WB
Species: Human

Verified Customer
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol RPN1
Entrez