Ribophorin I Antibody (OTI3H3)

Images

 
Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts ...read more
Immunocytochemistry/ Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.
Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells transfected with either Ribophorin I Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained ...read more
Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3H3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Ribophorin I Antibody (OTI3H3) Summary

Immunogen
Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPN1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Application Notes
ICC data from customer review.
Theoretical MW
66.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NBP2-01294 in the following applications:

Publications
Read Publication using
NBP2-01294 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Affinity purified

Alternate Names for Ribophorin I Antibody (OTI3H3)

  • DKFZp686B16177
  • dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit
  • Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
  • dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
  • EC 2.4.1.119
  • oligosaccharyltransferase 1 homolog
  • OST1
  • ribophorin IRBPH1
  • ribophorin-1
  • RPN-I

Background

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-46439
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP1-80835
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-48848
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37888
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00023263-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, S-ELISA
NBP2-38323
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-669
Species: Hu, Mu
Applications: WB, ICC/IF
MAB197
Species: Hu
Applications: Flow, IHC, CyTOF-reported, ICC, Neut
NBP1-92025
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-59845
Species: Hu
Applications: IP (-), WB, IHC, IHC-P
AF6295
Species: Hu, Mu
Applications: WB, IHC
NBP1-85525
Species: Hu
Applications: WB, IHC, IHC-P
AF3928
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO
NBP2-93776
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-06274
Species: Hu, Mu, Rt, Ce, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IHC-FrFl
NBP1-30447
Species: Hu
Applications: WB, IHC, IHC-P, IP
AF5129
Species: Hu
Applications: WB
H00029978-M03
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, KD
NBP1-56536
Species: Hu
Applications: WB, IHC, IHC-P

Publications for Ribophorin I Antibody (NBP2-01294)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Ribophorin I Antibody (NBP2-01294) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-01294:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Ribophorin I NBP2-01294
Enlarge
5
reviewed by:
Teddy Chan
WB Human 02/08/2019
View

Summary

ApplicationWestern Blot
Sample TestedBrain Vascular Smooth Muscle Cell lysate
SpeciesHuman
LotA01

Comments

CommentsBetter reactivity when samples are not boiled
Immunocytochemistry Ribophorin I NBP2-01294
Enlarge
4
reviewed by:
Anonymous
ICC Human 07/01/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman prostate cells tumor and non tumor
SpeciesHuman

Blocking

Blocking Details5% goat serum

Primary Anitbody

Dilution Ratio1/200 dilution, incubation-Over night at 4 degree

Secondary Antibody

Secondary DescriptionGoat anti mouse alexa flour 488 molecular probes (1/2000)
Secondary Concentration1/2000

Details

Detection Notes172 ms exposure time, Leica DMi3000b
Fixation Detailsfixed using 100% methnol at -20C
Wash Description5 minutes wash with PBS tween

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ribophorin I Antibody (NBP2-01294) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73898AF350
Alexa Fluor 405 NBP2-73898AF405
Alexa Fluor 488 NBP2-73898AF488
Alexa Fluor 532 NBP2-73898AF532
Alexa Fluor 594 NBP2-73898AF594
Alexa Fluor 647 NBP2-73898AF647
Alexa Fluor 700 NBP2-73898AF700
Alexa Fluor 750 NBP2-73898AF750
Allophycocyanin NBP2-73898APC
Biotin NBP2-73898B
DyLight 350 NBP2-73898UV
DyLight 405 NBP2-73898V
DyLight 488 NBP2-73898G
DyLight 550 NBP2-73898R
DyLight 594 NBP2-73898DL594
DyLight 650 NBP2-73898C
DyLight 680 NBP2-73898FR
DyLight 755 NBP2-73898IR
FITC NBP2-73898F
HRP NBP2-73898H
Janelia Fluor 549 NBP2-73898JF549
Janelia Fluor 646 NBP2-73898JF646
PE NBP2-73898PE
PerCP NBP2-73898PCP

Additional Ribophorin I Products

Bioinformatics Tool for Ribophorin I Antibody (NBP2-01294)

Discover related pathways, diseases and genes to Ribophorin I Antibody (NBP2-01294). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ribophorin I Antibody (NBP2-01294)

Discover more about diseases related to Ribophorin I Antibody (NBP2-01294).
 

Pathways for Ribophorin I Antibody (NBP2-01294)

View related products by pathway.

PTMs for Ribophorin I Antibody (NBP2-01294)

Learn more about PTMs related to Ribophorin I Antibody (NBP2-01294).

Blogs on Ribophorin I

There are no specific blogs for Ribophorin I, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Teddy Chan
02/08/2019
Application: WB
Species: Human

Anonymous
07/01/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol RPN1
Entrez