ESRP2 Antibody - BSA Free

Western Blot: ESRP2 Antibody [NBP1-76537] - Human lung tissue lysate with ESRP2 antibody at 1 ug/mL.

Immunohistochemistry: ESRP2 Antibody - BSA Free [NBP1-76537] - Immunohistochemistry of ESRP2 in human lung tissue with ESRP2 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: ESRP2 Antibody - BSA Free [NBP1-76537] - Immunofluorescence of ESRP2 in human lung tissue cells with ESRP2 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ESRP2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ESRP2 Antibody - BSA Free (NBP1-76537) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human RBM35B. The immunogen is located within the last 50 amino acids of RBM35B. Amino Acid Squence: PADDYTSLMPVGDPPRT
• Specificity: RBM35B antibody is predicted to not cross-react with other RBM35/ESRP family members. At least two isoforms of RBM35B are known to exist; this antibody will detect both isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ESRP2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  ESRP2 Peptide (NBP1-76537PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for ESRP2 Antibody - BSA Free

• epithelial splicing regulatory protein 2
• FLJ21918
• FLJ22248
• RBM35BRNA-binding protein 35B
• RNA binding motif protein 35A
• RNA binding motif protein 35B
• RNA-binding motif protein 35B

Background

RBM35A, also known as ESRP2, is a mRNA splicing factor that with its related protein RBM35A (ESRP1) are coordinators of an epithelial cell-type-specific splicing program. Both RBM35B and RBM35A are involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs, suggesting that these proteins are global regulators of an epithelial regulatory network. Loss of this global ESRP-regulated epithelial splicing programme induces the phenotypic changes in cell morphology that are observed during the epithelial-mesenchymal transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ESRP2
• Uniprot:
  • NP_079215(Human)