SF3B2 Antibody

Images

 
Independent Antibodies: Western Blot: SF3B2 Antibody [NB100-79848] - Detection of Human SF3b145/SAP145 from HeLa whole cell lysate (5, 15, 50 microgram) and 293T (50 microgram) using NB100-79848. SF3b145/SAP145 ...read more
Immunohistochemistry: SF3B2 Antibody [NB100-79848] - Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

SF3B2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 825 and 872 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SF3B2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-79848 in the following applications:

Publications
Read Publication using
NB100-79848 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SF3B2 Antibody

  • Cus1
  • pre-mRNA splicing factor SF3b 145 kDa subunit
  • Pre-mRNA-splicing factor SF3b 145 kDa subunit
  • SAP145SAP 145
  • SF3b1
  • SF3B145
  • SF3b150
  • spliceosome associated protein 145
  • Spliceosome-associated protein 145
  • splicing factor 3B subunit 2
  • splicing factor 3b, subunit 2, 145kD
  • splicing factor 3b, subunit 2, 145kDa

Background

This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SF3B2 Antibody (NB100-79848)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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WB
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Review for SF3B2 Antibody (NB100-79848) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-79848:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SF3B2 NB100-79848
Enlarge
reviewed by:
Astrid Spannhoff
WB Human 11/19/2010
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate, Sample Amount: 15-20 ul
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio: 1:2000, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit IgG, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9340

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 1 minute, Specific Bands: around 110 kDa

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Video Protocols

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FAQs for SF3B2 Antibody (NB100-79848) (0)

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Secondary Antibodies

 

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Recent Reviews

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Astrid Spannhoff
11/19/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol SF3B2
Entrez
Uniprot