Western Blot: SF3B2 Antibody [NB100-79848] - Detection of Human SF3b145/SAP145 from HeLa whole cell lysate (5, 15, 50 microgram) and 293T (50 microgram) using NB100-79848. SF3b145/SAP145 was also immunoprecipitated by ...read more
Immunohistochemistry: SF3B2 Antibody [NB100-79848] - Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). ...read more
The immunogen recognized by this antibody maps to a region between residue 825 and 872 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).
Immunogen affinity purified
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This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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