Independent Antibodies: Western Blot: SF3B2 Antibody [NB100-79848] - Detection of Human SF3b145/SAP145 from HeLa whole cell lysate (5, 15, 50 microgram) and 293T (50 microgram) using NB100-79848. SF3b145/SAP145 ...read more
Immunohistochemistry: SF3B2 Antibody [NB100-79848] - Sample: FFPE section of human linitis plastica stomach cancer. Antibody: Affinity purified rabbit anti- SF3b145/SAP145 used at a dilution of 1:200 (1ug/ml). ...read more
The immunogen recognized by this antibody maps to a region between residue 825 and 872 of human splicing factor 3b, subunit 2, 145kDa (spliceosome associated protein 145) using the numbering given in entry AAA97461.1 (GeneID 10992).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.