Independent Antibodies: Western Blot: hnRNP H Antibody [NB100-385] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NB100-385] - analysis of hnRNP H in SH-SY5Y cells using anti-hnRNP H antibody (green). Cells were fixed in 4% PFA in PBS for 15 min at room temperature. Image ...read more
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Simple Western: hnRNP H Antibody [NB100-385] - Simple Western lane view shows a specific band for hnRNP H in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
Rat (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. hnRNP H antibody validated for ICC/IFfrom a verified customer review.
49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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