hnRNP H Antibody

Images

 
Western Blot: hnRNP H Antibody [NB100-385] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NB100-385] - analysis of hnRNP H in SH-SY5Y cells using anti-hnRNP H antibody (green). Cells were fixed in 4% PFA in PBS for 15 min at room temperature. Image ...read more
Immunohistochemistry: hnRNP H Antibody [NB100-385] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-hnRNP-H at dilution of 1:250. DAB staining using anti-Rabbit IHC antibody at ...read more
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-hnRNP-H used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit ...read more
Simple Western: hnRNP H Antibody [NB100-385] - Simple Western lane view shows a specific band for hnRNP H in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

hnRNP H Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:30000
  • Simple Western 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 4-10 ug/mg of lysate
Application Notes
In Western Blot this antibody recognizes a band at ~49 kDa, representing hnRNP H.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC9)
hnRNP H Lysate (NBL1-11643)
Reviewed Applications
Read 1 Review rated 3
using
NB100-385 in the following applications:

Publications
Read Publications using
NB100-385 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hnRNP H Antibody

  • DKFZp686A15170
  • heterogeneous nuclear ribonucleoprotein H
  • heterogeneous nuclear ribonucleoprotein H1 (H)
  • hnRNP H
  • HNRPH
  • HNRPH1hnRNPH

Background

The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP H Antibody (NB100-385)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-385 Applications Species
Kieffer-Kwon P, Happel C, Uldrick TS et al. KSHV MicroRNAs Repress Tropomyosin 1 and Increase Anchorage-Independent Growth and Endothelial Tube Formation. PLoS ONE. 2015 Aug 12 [PMID: 26263384] (WB, Human) WB Human
Lee KY, Li M, Manchanda M et al. Compound loss of muscleblind-like function in myotonic dystrophy. EMBO Mol Med. 2013 Dec [PMID: 24293317] (WB, Mouse) WB Mouse
Vivarelli S, Lenzken SC, Ruepp MD et al. Paraquat Modulates Alternative Pre-mRNA Splicing by Modifying the Intracellular Distribution of SRPK2. PLoS One 2013 Apr 16 [PMID: 23613995] (WB, Human) WB Human
Wang E, Mueller WF, Hertel KJ et al. G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site. J Biol Chem 2011 Feb [PMID: 21127064]
Ling SC, Albuquerque CP, Han JS et al. ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS. Proc Natl Acad Sci U S A 2010 Jul [PMID: 20624952]
Van Dusen CM, Yee L, McNally LM et al. A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1. Mol Cell Biol 2010 May [PMID: 20308327]
Ohe K, Watanabe T, Harada S et al. Regulation of alternative splicing of the receptor for advanced glycation endproducts (RAGE) through G-rich cis-elements and heterogenous nuclear ribonucleoprotein H. J Biochem 2010 May [PMID: 20028692]
de Planell-Saguer M, Schroeder DG, Rodicio MC et al. Biochemical and genetic evidence for a role of IGHMBP2 in the translational machinery. Hum Mol Genet 2009 Jun [PMID: 19299493]
Wang E, Cambi F. Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs. J Biol Chem 2009 Apr [PMID: 19244236]
Jonson L, Vikesaa J, Krogh A et al. Molecular composition of IMP1 ribonucleoprotein granules. Mol Cell Proteomics 2007 May [PMID: 17289661]
Show All 14 Publications.

Review for hnRNP H Antibody (NB100-385) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB100-385:
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IF
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence hnRNP H NB100-385
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reviewed by:
Simona Darovic
IF Human 01/21/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSH-SY5Y cell culture
SpeciesHuman
LotA2

Blocking

Blocking Details10 % goat serum with 3 % BSA in PBS, 1hr, RT

Primary Anitbody

Dilution RatiohnRNP H Antibody NB100-385 Dilution, Dilution: 1:100; 1h; room temperature, 3 % BSA in PBS

Secondary Antibody

Secondary Descriptiongoat anti-rabbit Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Highly cross-adsorbed
Secondary Manufacturer Cat#Invitrogen A-11070
Secondary Concentration1:500

Details

Detection Notesconfocal microscope LSM710, Zeiss
Fixation DetailsFixation: 4 % PFA in PBS 15 min RT; Permeabilization: 0,1 %T riton in PBS 6 min RT
Wash DescriptionPBS, 5 min, 3 x

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP H Antibody (NB100-385) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Simona Darovic
01/21/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol HNRNPH1
Entrez
Uniprot