hnRNP H Antibody


Western Blot: hnRNP H Antibody [NB100-385] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit more
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NB100-385] - analysis of hnRNP H in SH-SY5Y cells using anti-hnRNP H antibody (green). Cells were fixed in 4% PFA in PBS for 15 min at room temperature. Image more
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - FFPE section of mouse squamous cell carcinoma. Affinity purified rabbit anti-hnRNP-H used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit more
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - FFPE section of human prostate adenocarcinoma. Affinity purified rabbit anti-hnRNP-H at dilution of 1:250. DAB staining using anti-Rabbit IHC antibody at more
Simple Western: hnRNP H Antibody [NB100-385] - Simple Western lane view shows a specific band for hnRNP H in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

Order Details

hnRNP H Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Rabbit (100%), Primate (100%), Equine (100%), Monkey (100%), Canine (100%), Guinea Pig (100%)
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-citrate/phosphate buffer, pH 7 to 8
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
In Western Blot this antibody recognizes a band at ~49 kDa, representing hnRNP H. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 1 Review rated 3
NB100-385 in the following application:

Read Publications using
NB100-385 in the following applications:

  • WB
    3 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, African elephant and Gray short-tailed opossum.

Alternate Names for hnRNP H Antibody

  • DKFZp686A15170
  • heterogeneous nuclear ribonucleoprotein H
  • heterogeneous nuclear ribonucleoprotein H1 (H)
  • hnRNP H


The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP H Antibody (NB100-385)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB100-385 Applications Species
Kieffer-Kwon P, Happel C, Uldrick TS et al. KSHV MicroRNAs Repress Tropomyosin 1 and Increase Anchorage-Independent Growth and Endothelial Tube Formation. PLoS ONE. 2015 Aug 12 [PMID:26263384] (WB, Human) WB Human
Lee KY, Li M, Manchanda M et al. Compound loss of muscleblind-like function in myotonic dystrophy. EMBO Mol Med. 2013 Dec [PMID:24293317] (WB, Mouse) WB Mouse
Vivarelli S, Lenzken SC, Ruepp MD et al. Paraquat Modulates Alternative Pre-mRNA Splicing by Modifying the Intracellular Distribution of SRPK2. PLoS One 2013 Apr 16 [PMID:23613995] (WB, Human) WB Human
Wang E, Mueller WF, Hertel KJ et al. G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site. J Biol Chem 2011 Feb [PMID:21127064]
Ling SC, Albuquerque CP, Han JS et al. ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS. Proc Natl Acad Sci U S A 2010 Jul [PMID:20624952]
Van Dusen CM, Yee L, McNally LM et al. A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1. Mol Cell Biol 2010 May [PMID:20308327]
Ohe K, Watanabe T, Harada S et al. Regulation of alternative splicing of the receptor for advanced glycation endproducts (RAGE) through G-rich cis-elements and heterogenous nuclear ribonucleoprotein H. J Biochem 2010 May [PMID:20028692]
de Planell-Saguer M, Schroeder DG, Rodicio MC et al. Biochemical and genetic evidence for a role of IGHMBP2 in the translational machinery. Hum Mol Genet 2009 Jun [PMID:19299493]
Wang E, Cambi F. Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs. J Biol Chem 2009 Apr [PMID:19244236]
Jonson L, Vikesaa J, Krogh A et al. Molecular composition of IMP1 ribonucleoprotein granules. Mol Cell Proteomics 2007 May [PMID:17289661]
Show All 14 Publications.

Reviews for hnRNP H Antibody (NB100-385) (1) 31

Average Rating: 3
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.

Reviews using NB100-385:
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Images Ratings Applications Species Date Details
 hnRNP H NB100-385
reviewed by:
Human 01/21/2015


Sample TestedSH-SY5Y cell culture


Blocking Details10 % goat serum with 3 % BSA in PBS, 1hr, RT

Primary Anitbody

Dilution RatiohnRNP H Antibody NB100-385 Dilution, Dilution: 1:100; 1h; room temperature, 3 % BSA in PBS

Secondary Antibody

Secondary Descriptiongoat anti-rabbit Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) Highly cross-adsorbed
Secondary Manufacturer Cat#Invitrogen A-11070
Secondary Concentration1:500


Detection Notesconfocal microscope LSM710, Zeiss
Fixation DetailsFixation: 4 % PFA in PBS 15 min RT; Permeabilization: 0,1 %T riton in PBS 6 min RT
Wash DescriptionPBS, 5 min, 3 x

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for hnRNP H Antibody (NB100-385) (0)

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Positive Control Lysate(s)

Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol HNRNPH1

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