hnRNP H Antibody

Images

 
Independent Antibodies: Western Blot: hnRNP H Antibody [NB100-385] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. ...read more
Immunocytochemistry/ Immunofluorescence: hnRNP H Antibody [NB100-385] - analysis of hnRNP H in SH-SY5Y cells using anti-hnRNP H antibody (green). Cells were fixed in 4% PFA in PBS for 15 min at room temperature. Image ...read more
Immunohistochemistry-Paraffin: hnRNP H Antibody [NB100-385] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- hnRNP H used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Simple Western: hnRNP H Antibody [NB100-385] - Simple Western lane view shows a specific band for hnRNP H in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

hnRNP H Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 400 and the C-terminus (residue 448) of human Heterogeneous Nuclear Ribonucleoprotein H1 using the numbering given in entry NP_005511.1 (GeneID 3187).
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HNRNPH1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 4-10 ug/mg of lysate
  • Simple Western 1:1000
  • Western Blot 1:10000-1:30000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. hnRNP H antibody validated for ICC/IFfrom a verified customer review.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
hnRNP H Overexpression Lysate
Publications
Read Publications using
NB100-385 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for hnRNP H Antibody

  • DKFZp686A15170
  • heterogeneous nuclear ribonucleoprotein H
  • heterogeneous nuclear ribonucleoprotein H1 (H)
  • hnRNP H
  • HNRPH
  • HNRPH1hnRNPH

Background

The heterogeneous nuclear Ribonucleoprotein (hnRNP) H protein family is involved in pre-mRNA processing. It is essential for enhancer activity. The sequence GGGA is recognized by all hnRNP H family proteins. Mutant exon 6D sequences function as a splicing enhancer when inserted into an enhancer-dependent splicing construct. hnRNP H family proteins bind to the enhancer and their binding is dependent on the sequence GGGA, located just downstream of the point of mutation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for hnRNP H Antibody (NB100-385)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(4)
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Human
(3)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-385 Applications Species
Li Y, Bakke J, Finkelstein D et al. HNRNPH1 is required for rhabdomyosarcoma cell growth and survival Oncogenesis 2018 Jan 24 [PMID: 29362363] (WB, Human) WB Human
Kieffer-Kwon P, Happel C, Uldrick TS et al. KSHV MicroRNAs Repress Tropomyosin 1 and Increase Anchorage-Independent Growth and Endothelial Tube Formation. PLoS ONE. 2015 Aug 12 [PMID: 26263384] (WB, Human) WB Human
Lee KY, Li M, Manchanda M et al. Compound loss of muscleblind-like function in myotonic dystrophy. EMBO Mol Med. 2013 Dec [PMID: 24293317] (WB, Mouse) WB Mouse
Vivarelli S, Lenzken SC, Ruepp MD et al. Paraquat Modulates Alternative Pre-mRNA Splicing by Modifying the Intracellular Distribution of SRPK2. PLoS One 2013 Apr 16 [PMID: 23613995] (WB, Human) WB Human
Wang E, Mueller WF, Hertel KJ et al. G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site. J Biol Chem 2011 Feb [PMID: 21127064]
Ling SC, Albuquerque CP, Han JS et al. ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS. Proc Natl Acad Sci U S A 2010 Jul [PMID: 20624952]
Van Dusen CM, Yee L, McNally LM et al. A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1. Mol Cell Biol 2010 May [PMID: 20308327]
Ohe K, Watanabe T, Harada S et al. Regulation of alternative splicing of the receptor for advanced glycation endproducts (RAGE) through G-rich cis-elements and heterogenous nuclear ribonucleoprotein H. J Biochem 2010 May [PMID: 20028692]
de Planell-Saguer M, Schroeder DG, Rodicio MC et al. Biochemical and genetic evidence for a role of IGHMBP2 in the translational machinery. Hum Mol Genet 2009 Jun [PMID: 19299493]
Wang E, Cambi F. Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs. J Biol Chem 2009 Apr [PMID: 19244236]
Show All 15 Publications.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HNRNPH1
Entrez
Uniprot