ESRP1/2 Antibody (23A7.C9) - BSA Free

Western Blot: ESRP1/2 Antibody (23A7.C9) [NBP1-77971] - 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 23A7.C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

ESRP1/2 Antibody (23A7.C9) - BSA Free Summary

• Description: This antibody was purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: ESRP1/2 Antibody (23A7.C9) was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein. (Uniprot: Q8K0G8)
• Specificity: This antibody reacts with both mouse ESRP1/2 and Esrp-2 proteins. A BLAST analysis of the immunizing protein sequence shows 100% homology with ESRP1/2 from mouse and a 91% sequence homology with ESRP1/2 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ESRP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:750000
  • Western Blot 1:1000
• Application Notes: This protein-A purified antibody has been tested for use western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 75.5 kDa in size corresponding to Esrp-1 and 77.4 kDa in size corresponding to Esrp-2 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis of the immunizing protein sequence shows 100% homology with ESRP1/2 from mouse and a 91% sequence homology with ESRP1/2 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Use in Human reported in scientific literature (PMID: 33500360)

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for ESRP1/2 Antibody (23A7.C9) - BSA Free

• epithelial splicing regulatory protein 2,9530027K23Rik
• Rbm35b
• RNA binding motif protein 35b
• RNA-binding motif protein 35B
• RNA-binding protein 35B

Background

Epithelial splicing regulatory protein-1 (Esrp-1) and Esrp-2 are mRNA splicing factors that regulate the formation of epithelial cell-specific isoforms. They specifically regulate the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-1 and -2 act by directly binding specific sequences in mRNAs. They bind the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ESRP1/2 Antibody (NBP1-77971)(3)

Publications using NBP1-77971

• Ye Q, Mohamed R, Dukhlallah D et al. Molecular Analysis of ZNF71 KRAB in Non-Small-Cell Lung Cancer International Journal of Molecular Sciences 2021-04-04 [PMID: 33916522] (WB, Human)
• Addison JB, Voronkova MA, Fugett JH, et al. Functional hierarchy and cooperation of EMT master transcription factors in breast cancer metastasis Molecular cancer research : MCR 2021-01-26 [PMID: 33500360] (WB, Human)
• Warzecha CC, Jiang P, Amirikian K et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 2010-10-01 [PMID: 20711167]

Bioinformatics

• Gene Symbol: ESRP1
• Uniprot:
  • NP_918944.2(Mouse)
  • Q8K0G8(Mouse)