Erythropoietin R Antibody

Images

 
Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis of the EPO receptor in human placenta.
Simple Western: Erythropoietin R Antibody [NBP1-19388] - Simple Western lane view shows a specific band for EPO Receptor in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Erythropoietin R Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human EPO receptor protein (between residues 300-400) [UniProt P19235]
Localization
Membrane
Specificity
EpoR (A364) detects endogenous levels of EpoR protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPOR
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2.0 ug/ml
  • Simple Western 1:10
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-19388 in the following applications:

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Erythropoietin R Antibody

  • EpoR
  • EPO-R
  • Erythropoietin R
  • erythropoietin receptor
  • MGC138358

Background

The erythropoietin receptor (EPOR) is a cytokine receptor family member. There are three known isoforms of the receptor, EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). When bound to erythropoietin (EPO), EPOR is phosphorylated by Jak2 tyrosine kinase. Phosphorylated EPOR serves as the docking site for a number of different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPOR-Stat5 signaling appears to play a role in erythroid cell survival. Animal studies have shown that truncated EPOR that can still be bound by Jak2 are viable, suggesting that basal Jak2 activity is sufficient for erythropoiesis Stat5 binding is important, as a mutated Tyr343 on EPOR results in anemia and poor response to hypoxic stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Erythropoietin R Antibody (NBP1-19388) (0)

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Review for Erythropoietin R Antibody (NBP1-19388) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-19388:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Erythropoietin R NBP1-19388
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reviewed by:
zhang meng
WB Mouse 11/17/2017
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Summary

ApplicationWestern Blot
Sample Testedblood vessel tissue
SpeciesMouse
LotA-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Erythropoietin R Antibody (NBP1-19388). (Showing 1 - 1 of 1 FAQ).

  1. EPO Receptor Antibody (NBP1-19388) is suitable for immunohistochemistry (paraffin) and you also say that it can be used for IF. I just want to confirm that this particular antibody can be visualized by other systems also?
    • This antibody has only been validated for use in WB and IHC-Paraffin, not Immunofluorescence. This was incorrect information on the datasheet and I have now corrected this.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NBP1-19388AF488
Alexa Fluor 647 Labeled NBP1-19388AF647
Biotin Labeled NBP1-19388B
DyLight 350 Labeled NBP1-19388UV
DyLight 405 Labeled NBP1-19388V
DyLight 488 Labeled NBP1-19388G
DyLight 550 Labeled NBP1-19388R
DyLight 650 Labeled NBP1-19388C
DyLight 680 Labeled NBP1-19388FR
DyLight 755 Labeled NBP1-19388IR
HRP Labeled NBP1-19388H

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Recent Reviews

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zhang meng
11/17/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EPOR
Entrez
Uniprot