Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis of the EPO receptor in human placenta.
Simple Western: Erythropoietin R Antibody [NBP1-19388] - Simple Western lane view shows a specific band for EPO Receptor in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Erythropoietin R Antibody
The erythropoietin receptor (EPOR) is a cytokine receptor family member. There are three known isoforms of the receptor, EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). When bound to erythropoietin (EPO), EPOR is phosphorylated by Jak2 tyrosine kinase. Phosphorylated EPOR serves as the docking site for a number of different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPOR-Stat5 signaling appears to play a role in erythroid cell survival. Animal studies have shown that truncated EPOR that can still be bound by Jak2 are viable, suggesting that basal Jak2 activity is sufficient for erythropoiesis Stat5 binding is important, as a mutated Tyr343 on EPOR results in anemia and poor response to hypoxic stress.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Erythropoietin R Antibody (NBP1-19388). (Showing 1 - 1 of 1 FAQs).
EPO Receptor Antibody (NBP1-19388) is suitable for immunohistochemistry (paraffin) and you also say that it can be used for IF. I just want to confirm that this particular antibody can be visualized by other systems also?
This antibody has only been validated for use in WB and IHC-Paraffin, not Immunofluorescence. This was incorrect information on the datasheet and I have now corrected this.
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