Erythropoietin R Antibody - BSA Free

Images

 
Western Blot: Erythropoietin R Antibody [NBP1-19388] - Western blot analysis of K562 (left) and SH-SY5Y (right) cell lysate using EPO receptor antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Erythropoietin R Antibody [NBP1-19388] - EPO receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) ...read more
Immunohistochemistry-Paraffin: Erythropoietin R Antibody [NBP1-19388] - IHC analysis of the EPO receptor in human placenta.
Simple Western: Erythropoietin R Antibody [NBP1-19388] - Simple Western lane view shows a specific band for EPO Receptor in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Erythropoietin R Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human EPO receptor protein (between residues 300-400) [UniProt P19235]
Localization
Membrane
Specificity
EpoR (A364) detects endogenous levels of EpoR protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EPOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:50 - 1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Simple Western 1:10
  • Western Blot 2.0 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-19388 in the following applications:

Publications
Read Publications using
NBP1-19388 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34552120). Human, Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Erythropoietin R Antibody - BSA Free

  • EpoR
  • EPO-R
  • Erythropoietin R
  • erythropoietin receptor
  • MGC138358

Background

The erythropoietin receptor (EPOR) is a cytokine receptor family member. There are three known isoforms of the receptor, EPOR-F (full length), EPOR-S (soluble form), and EPOR-T (truncated form). When bound to erythropoietin (EPO), EPOR is phosphorylated by Jak2 tyrosine kinase. Phosphorylated EPOR serves as the docking site for a number of different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. EPOR-Stat5 signaling appears to play a role in erythroid cell survival. Animal studies have shown that truncated EPOR that can still be bound by Jak2 are viable, suggesting that basal Jak2 activity is sufficient for erythropoiesis Stat5 binding is important, as a mutated Tyr343 on EPOR results in anemia and poor response to hypoxic stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for Erythropoietin R Antibody (NBP1-19388)(3)

Reviews for Erythropoietin R Antibody (NBP1-19388) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-19388:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Erythropoietin R NBP1-19388
Enlarge
5
reviewed by:
Daiana Drehmer
WB Human 10/05/2022
View

Summary

ApplicationWestern Blot
Sample Testedk562 cells
SpeciesHuman
LotA-1
Western Blot Erythropoietin R NBP1-19388
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 11/17/2017
View

Summary

ApplicationWestern Blot
Sample Testedblood vessel tissue
SpeciesMouse
LotA-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Erythropoietin R Antibody (NBP1-19388). (Showing 1 - 1 of 1 FAQs).

  1. EPO Receptor Antibody (NBP1-19388) is suitable for immunohistochemistry (paraffin) and you also say that it can be used for IF. I just want to confirm that this particular antibody can be visualized by other systems also?
    • This antibody has only been validated for use in WB and IHC-Paraffin, not Immunofluorescence. This was incorrect information on the datasheet and I have now corrected this.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Erythropoietin R Antibody (NBP1-19388)

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Daiana Drehmer
10/05/2022
Application: WB
Species: Human

Verified Customer
11/17/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EPOR
Entrez
Uniprot