Thrombopoietin R/Tpo R Antibody

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Recombinant Human Thrombopoietin/Tpo (Catalog # 288-TP) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Thrombopoietin R/Tpo R Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombopoietin R/Tpo R
Gln26-Tyr423
Accession # P40238
Specificity
Detects human Thrombopoietin R/Tpo R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human Epo R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MPL
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 0.5-2.5 ug/mL
Publications
Read Publications using
AF1016 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombopoietin R/Tpo R Antibody

  • CD110 antigen
  • CD110
  • c-Mpl
  • MPL
  • MPLV
  • Myeloproliferative leukemia protein
  • myeloproliferative leukemia virus oncogene
  • Proto-oncogene c-Mpl
  • Thrombopoietin R
  • thrombopoietin receptor
  • TpoR
  • TPO-R
  • TPORMPLV

Background

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a 95 kDa type I transmembrane protein that is a member of the type I cytokine receptor family within the hematopoietin/cytokine receptor superfamily (1‑4). The 635 amino acid (aa) full-length human Tpo R contains a 25 aa signal sequence, a 466 aa extracellular domain with a ligand binding domain and two fibronectin type III domains, a transmembrane (TM) domain and a cytoplasmic domain. The extracellular domain of human Tpo R shares 78%, 76%, 81%, 82% and 80% aa identity with mouse, rat, bovine, canine and equine Tpo R, respectively. Humans produce three distinct mRNA species; a P-form, a K-form, and a truncated form (Mpl-tr) lacking a TM domain (3‑7). The P-form encodes the full-length receptor. The Mpl-tr form, which is expressed in both human and mouse, is intracellular and targets the P-form for degradation (5, 6). The 579 aa K-form has an alternate cytoplasmic domain, but does not dimerize with, or inhibit, the P-form (7). Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis, thrombopoiesis and hematopoietic stem cell self-renewal, as reflected by expression of the Tpo R on megakaryocytes, platelets and hematopoietic progenitors (2, 8). Receptor dimerization occurs upon Tpo binding and initiates signaling through the Ras/MAP and JAK/STAT pathways (1, 2). Internalization and degradation of Tpo following Tpo R binding serves to downregulate circulating Tpo (9). Tpo R expressed at low levels on endothelial cells does not appear to contribute to regulation of Tpo (10). Inactivating mutations of Tpo R cause thrombocytopenia, and absence of functional Tpo R is lethal in humans, but not mice. Other mutations, including an activating change in the TM domain, can cause thrombocytosis (11, 12).

  1. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  2. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453. 
  3. Vigon, I. et al. (1992) Proc. Natl. Acad. Sci. USA 89:5640. 
  4. Mignotte, V. et al. (1994) Genomics 20:5. 
  5. Li, J. et al. (2000) Cytokine 12:835. 
  6. Coers, J. et al. (2004) J. Biol. Chem. 279:36397. 
  7. Millot, G.A. et al. (2002) Exp. Hematol. 30:166.
  8. Tong, W. et al. (2007) Exp. Hematol. July 14 [Epub ahead of print].
  9. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  10. Geddis, A.E. et al. (2006) Exp. Hematol. 34:82.
  11. Germeshausen, M. et al. (2006) Hum. Mutat. 27:296.
  12. Ding, J. et al. (2004) Blood 103:4198.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MPL
Uniprot