Erythropoietin R Antibodies

HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Erythropoietin R at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB0041, open histogram) followed by anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> MAB0041, open histogram) followed by anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

HAF109). Specific bands were detected for Erythropoietin R at approximately 70-78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF109). Specific bands were detected for Erythropoietin R at approximately 70-78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-322-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Erythropoietin R at approximately 70-78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Erythropoietin R was detected in immersion fixed mouse bone marrow cells using Goat Anti-Mouse Erythropoietin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1390) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

287-TC) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 64 to 70 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (lower panel, Catalog # MAB3072). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 287-TC) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 64 to 70 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (lower panel, Catalog # MAB3072). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of UT-7 human acute myeloid leukemia cell line untreated (-) or treated (+) with 300 ng/mL Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 64 to 70 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (lower panel, Catalog # MAB3072). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

287-TC) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 70 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> 287-TC) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Erythropoietin R (Y426) at approximately 70 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Phospho-Erythropoietin R (Y426) Monoclonal Antibody (Catalog # MAB6926). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of UT-7 human acute myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Erythropoietin R at approximately 64 to 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

307-ER) was serially diluted and captured by Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3071) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Erythropoietin R Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 307-ER) was serially diluted and captured by Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3071) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Erythropoietin R Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human Erythropoietin R (Catalog # 307-ER) was serially diluted and captured by Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3071) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Erythropoietin R Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

287-TC) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND₅₀ is typically 0.4-2.4 µg/mL." class="big_lightbox"> 287-TC) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND₅₀ is typically 0.4-2.4 µg/mL." alt="Recombinant Human Erythropoietin (Tissue Culture Grade) (Catalog # 287-TC) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human Erythropoietin (Tissue Culture Grade) (0.278 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB3073). The ND₅₀ is typically 0.4-2.4 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="Erythropoietin R was detected in immersion fixed K562 human myelogenous leukemia cell line using Mouse Anti-Human Erythropoietin R Monoclonal Antibody (Catalog # MAB11190) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />