Endoglin/CD105 Antibody (SN6) [FITC]

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Flow Cytometry: Endoglin/CD105 Antibody (SN6) [FITC] [NB100-63330] - Staining of KG1 cells with MOUSE ANTI HUMAN CD105:FITC

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC-Fr, IP
Clone
SN6
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC
Concentration
0.1 mg/ml

Endoglin/CD105 Antibody (SN6) [FITC] Summary

Immunogen
Partially purified cell membrane antigens from fresh leukemia cells.
Marker
Neo-endothelial Cells Marker
Specificity
Recognizes the human CD105 cell surface antigen, a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukemia cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.
Positive Control
Endoglin/CD105 Lysate (NBL1-10266)

Reactivity Notes

Cross reacts with Horse, Cynomolgus Monkey, Rhesus Monkey, Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein G purified

Alternate Names for Endoglin/CD105 Antibody (SN6) [FITC]

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Endoglin/CD105 Antibody (NB100-63330). (Showing 1 - 2 of 2 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ENG
Entrez