Endoglin/CD105 Antibody (MEM-229) [FITC]

• Reactivity: Hu, Po, Ca(-), Eq(-)
• Applications: Flow, ICC/IF
• Clone: MEM-229
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

Endoglin/CD105 Antibody (MEM-229) [FITC] Summary

• Immunogen: recombinant vaccinia virus containing human CD105 (L-isoform) cDNA
• Marker: Neo-endothelial Cells Marker
• Specificity: This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENG
• Purity: Size Exclusion Chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
• Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Endoglin/CD105 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Stabilizing phosphate buffered saline (PBS).
• Preservative: 15mM Sodium Azide
• Purity: Size Exclusion Chromatography

Alternate Names for Endoglin/CD105 Antibody (MEM-229) [FITC]

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbR-2 as a receptor for TGFb-1 and TGFb-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFb-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglin/CD105 Antibody (NB110-81749)(2)

Publications using NB110-81749

• Dey D, Fischer NG, Dragon AH Et al. Culture and characterization of various porcine integumentary-connective tissue-derived mesenchymal stromal cells to facilitate tissue adhesion to percutaneous metal implants Stem Cell Res Ther 2021-12-19 [PMID: 34922628] (FLOW, Porcine)
  
  Details:
  Citation using the FITC version of this antibody.
• Planka L, Necas A, Srnec R et al. Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs Physiol Res 2009-01-01 [PMID: 19093735]
  
  Details:
  This citation used the Biotin version of this antibody.

FAQs for Endoglin/CD105 Antibody (NB110-81749). (Showing 1 - 2 of 2 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: ENG