Endoglin/CD105 Antibody (MEM-229)

Images

 
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PerCP-CyTM5.5.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with ...read more
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (MEM-229) NB110-58718PE (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) PE.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of CD105 on Huvec cells with anti-CD105 (MEM-229) biotin.

Product Details

Summary
Reactivity Hu, Po, Ca(-), Eq(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, CyTOF-ready
Clone
MEM-229
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Endoglin/CD105 Antibody (MEM-229) Summary

Immunogen
recombinant vaccinia virus containing human CD105 (L-isoform) cDNA
Marker
Neo-endothelial Cells Marker
Specificity
This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ENG
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • CyTOF-ready
Application Notes
Western Blot: use non-reducing conditions. This antibody is CyTOF ready.
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Endoglin/CD105 Overexpression Lysate
Endoglin/CD105 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB110-58718 in the following applications:

  • 2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Endoglin/CD105 Antibody (MEM-229)

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Po, Ca(-), Eq(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, CyTOF-ready

Publications for Endoglin/CD105 Antibody (NB110-58718)(3)

Reviews for Endoglin/CD105 Antibody (NB110-58718) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NB110-58718). (Showing 1 - 2 of 2 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-58718AF350
Alexa Fluor 405 NB110-58718AF405
Alexa Fluor 488 NB110-58718AF488
Alexa Fluor 532 NB110-58718AF532
Alexa Fluor 594 NB110-58718AF594
Alexa Fluor 647 NB110-58718AF647
Alexa Fluor 700 NB110-58718AF700
Alexa Fluor 750 NB110-58718AF750
Allophycocyanin NB110-58718APC
Allophycocyanin/Cy7 NB110-58718APCCY7
DyLight 350 NB110-58718UV
DyLight 405 NB110-58718V
DyLight 488 NB110-58718G
DyLight 550 NB110-58718R
DyLight 594 NB110-58718DL594
DyLight 650 NB110-58718C
DyLight 680 NB110-58718FR
DyLight 755 NB110-58718IR
FITC NB110-81749
Janelia Fluor 549 NB110-58718JF549
Janelia Fluor 646 NB110-58718JF646
PE NB110-58718PE
PE/Atto594 NB110-58718PEATT594
PE/Cy5.5 NB110-58718PECY55
PE/Cy7 NB110-58718PECY7
PerCP NB110-58718PCP

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Bioinformatics

Gene Symbol ENG