Endoglin/CD105 Antibody (MEM-229)

Images

 
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (MEM-229) NB110-58718PE (blue) and a matched isotype control (orange). ...read more
Flow Cytometry: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Analysis of the APC conjugate of this antibody. A cell surface stain was performed on THP-1 cells with Endoglin antibody NB110-58718APC (blue) along with ...read more
Immunofluorescence: Endoglin/CD105 Antibody (MEM-229) [NB110-58718] - Staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue).

Product Details

Summary
Reactivity Hu, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, CyTOF-ready, IF
Clone
MEM-229
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Endoglin/CD105 Antibody (MEM-229) Summary

Immunogen
recombinant vaccinia virus containing human CD105 (L-isoform) cDNA
Marker
Neo-endothelial Cells Marker
Specificity
This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ENG
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • CyTOF-ready
  • Immunofluorescence
Application Notes
Western Blot: use non-reducing conditions.
Positive Control
Endoglin/CD105 Lysate (NBL1-10266)
Publications
Read Publications using NB110-58718.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Endoglin/CD105 Antibody (MEM-229)

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF770
Species: Mu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-1642
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP, Flow-CS
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA
NB100-77417
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo, CyTOF-ready
AF2067
Species: Hu, Po, Ca
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-36561
Species: Hu, Mu
Applications: WB, B/N, ELISA, Flow, ICC/IF, IHC-Fr, IP
NBP2-56244
Species: Hu
Applications: WB, ICC/IF
AF1172
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-33050
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB110-58718
Species: Hu, Po
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, CyTOF-ready, IF

Publications for Endoglin/CD105 Antibody (NB110-58718)(3)

We have publications tested in 1 application: Flow.


Filter By Application
Flow
(1)
All Applications
Filter By Species
All Species

Reviews for Endoglin/CD105 Antibody (NB110-58718) (0)

There are no reviews for Endoglin/CD105 Antibody (NB110-58718). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NB110-58718). (Showing 1 - 2 of 2 FAQs).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-58718AF405
Alexa Fluor 488 Labeled NB110-58718AF488
Alexa Fluor 647 Labeled NB110-58718AF647
Alexa Fluor 700 Labeled NB110-58718AF700
Allophycocyanin Labeled NB110-58718APC
Biotin Labeled NBP1-97565
DyLight 350 Labeled NB110-58718UV
DyLight 405 Labeled NB110-58718V
DyLight 488 Labeled NB110-58718G
DyLight 680 Labeled NB110-58718FR
DyLight 755 Labeled NB110-58718IR
FITC Labeled NB110-81749
PE Labeled NB110-58718PE
PerCP Labeled NB110-58718PCP

Additional Endoglin/CD105 Products

Bioinformatics Tool for Endoglin/CD105 Antibody (NB110-58718)

Discover related pathways, diseases and genes to Endoglin/CD105 Antibody (NB110-58718). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Endoglin/CD105 Antibody (NB110-58718)

Discover more about diseases related to Endoglin/CD105 Antibody (NB110-58718).
 

Pathways for Endoglin/CD105 Antibody (NB110-58718)

View related products by pathway.

PTMs for Endoglin/CD105 Antibody (NB110-58718)

Learn more about PTMs related to Endoglin/CD105 Antibody (NB110-58718).
 

Research Areas for Endoglin/CD105 Antibody (NB110-58718)

Find related products by research area.

Blogs on Endoglin/CD105

There are no specific blogs for Endoglin/CD105, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Endoglin/CD105 Antibody (MEM-229) and receive a gift card or discount.

Bioinformatics

Gene Symbol ENG
Entrez