Endoglin/CD105 Antibody (3A9)

Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining.

Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysates.

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Analysis of HepG2 cells using CD105 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

ELISA: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 3A9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Endoglin/CD105 Antibody (3A9) Summary

• Immunogen: Purified recombinant fragment of human CD105 expressed in E. coli. [Uniprot: P17813]
• Marker: Neo-endothelial Cells Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENG
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Application Notes: ICC/IF reported in a verified customer review.
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Endoglin/CD105 Knockout HeLa Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27322553).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Endoglin/CD105 Antibody (3A9)

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglin/CD105 Antibody (NBP2-22122)(2)

Publications using NBP2-22122

• Esteban-Vives R, Ziembicki J, Choi MS et al. Isolation and Characterization of a Human Fetal Mesenchymal Stem Cell Population: Exploring the Potential for Cell Banking in Wound Healing Therapies Cell Transplant Aug 13 2019 [PMID: 31407589] (ICC/IF, Human)
• D'Amico L, Belisario D, Migliardi G et al. C-met inhibition blocks bone metastasis development induced by renal cancer stem cells Oncotarget Jul 19 2016 [PMID: 27322553] (IHC-P, Mouse)

Review for Endoglin/CD105 Antibody (NBP2-22122) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous ICC Human 01/07/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: HMSC cells
• Species: Human

Blocking

• Blocking Details: -

Primary Anitbody

• Dilution Ratio: 1 ug/ml, incubated for 16 hours at 4C

Secondary Antibody

• Secondary Description: donkey anti-mouse Alexa 488
• Secondary Concentration: 1:1,000

Details

• Detection Notes: -
• Fixation Details: ice-cold 1% or 2% formaldehyde for 5 min

FAQs for Endoglin/CD105 Antibody (NBP2-22122). (Showing 1 - 4 of 4 FAQs).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
3. I would like to know the concentration and purity of this antibody, and whether you could provide a purified format of this anti-CD105.
   • This product is provided as ammonium sulfate precipitated ascites. The concentration of this preparation is 1.0mg/ml (total IgG). Unfortunately, this product is not available in any other format at this time as the clone is licensed from a collaborator.
4. Regarding the anti-endoglin antiboy clone 3A9, what antigen sequence was it raised against?
   • Our product NBP2-22122 was raised against an immunogen that falls between amino acids 330-600 of the human Endoglin protein 

Bioinformatics

• Gene Symbol: ENG
• Uniprot:
  • P17813()