Endoglin/CD105 Antibody (3A9)

Images

 
Western Blot: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody ...read more
Immunocytochemistry: Endoglin/CD105 Antibody (3A9) [NBP2-22122] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: CD105 Antibody (3A9) [NBP2-22122] - Immunohistochemical analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB ...read more
Western Blot: CD105 Antibody (3A9) [NBP2-22122] - Western blot analysis using CD105 mAb against HEK293 (1) and CD105 (AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: CD105 Antibody (3A9) [NBP2-22122] - Immunofluorescence analysis of HepG2 cells using CD105 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
ELISA: CD105 Antibody (3A9) [NBP2-22122] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Endoglin/CD105 Antibody (3A9) Summary

Immunogen
Purified recombinant fragment of human CD105 expressed in E. coli. [UniProt# P17813]
Marker
Neo-endothelial Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENG
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This CD105 (3A9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22122 in the following applications:

Publications
Read Publication using
NBP2-22122 in the following applications:

Reactivity Notes

Human. Mouse reactivity reported in scientific literature (PMID: 27322553).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Endoglin/CD105 Antibody (3A9)

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endoglin/CD105 Antibody (NBP2-22122)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Mouse
(1)
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Review for Endoglin/CD105 Antibody (NBP2-22122) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-22122:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Endoglin/CD105 NBP2-22122
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reviewed by:
Anonymous
ICC Human 01/07/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedHMSC cells
SpeciesHuman

Blocking

Blocking Details-

Primary Anitbody

Dilution Ratio1 ug/ml, incubated for 16 hours at 4C

Secondary Antibody

Secondary Descriptiondonkey anti-mouse Alexa 488
Secondary Concentration1:1,000

Details

Detection Notes-
Fixation Detailsice-cold 1% or 2% formaldehyde for 5 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NBP2-22122). (Showing 1 - 4 of 4 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.
  3. I would like to know the concentration and purity of this antibody, and whether you could provide a purified format of this anti-CD105.
    • This product is provided as ammonium sulfate precipitated ascites. The concentration of this preparation is 1.0mg/ml (total IgG). Unfortunately, this product is not available in any other format at this time as the clone is licensed from a collaborator.
  4. Regarding the anti-endoglin antiboy clone 3A9, what antigen sequence was it raised against?
    • Our product NBP2-22122 was raised against an immunogen that falls between amino acids 330-600 of the human Endoglin protein 

Secondary Antibodies

 

Isotype Controls

Additional Endoglin/CD105 Products

Bioinformatics Tool for Endoglin/CD105 Antibody (NBP2-22122)

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Diseases for Endoglin/CD105 Antibody (NBP2-22122)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
01/07/2016
Application: ICC
Species: Human

Bioinformatics

Gene Symbol ENG
Entrez
Uniprot