ELF2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ELF2 Antibody [NBP1-84770] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Staining of human cerebral cortex, gastrointestinal, placenta and testis using Anti-ELF2 antibody HPA006057 (A) shows similar protein distribution across tissues to independent antibody HPA071166 (B).

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Lane 1: Marker [kDa] 229, 112, 84, 48, 32, 27, 17Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human rectum shows strong nuclear positivity in glandular cells.

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ELF2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: MTSAVVDSGGTILELSSNGVENQEESEKVSEYPAVIVEPVPSARLEQGYAAQVLVYDDETYMMQDVAEEQEVETENVETVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPEFIHAAMRPDVITETVVEVSTEESEP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ELF2 Protein (NBP1-84770PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ELF2 Antibody - BSA Free

• b
• E74-like factor 2 (ets domain transcription factor)
• E74-like factor 2
• ets family transcription factor ELF2C
• ETS-related transcription factor Elf-2
• NERF-1a
• NERF-1B
• NERF-2
• NERFEU32
• New ETS-related factor

Background

soform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter .Isoform 2 may function in repression of RUNX1-mediated transactivation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ELF2 Antibody (NBP1-84770)(1)

Publication using NBP1-84770

• Fausther M, Lavoie EG, Goree JR, Dranoff JA. An Elf2-like transcription factor acts as repressor of the mouse ecto-5'-nucleotidase gene expression in hepatic myofibroblasts Purinergic Signal. 2017-06-30 [PMID: 28667437] (Mouse)

Bioinformatics

• Gene Symbol: ELF2