ELF3/ESE-1 Antibody

Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Loss of Elf3 preserves the mesenchymal state. Immunoblot showing ELF3 and E-cadherin protein levels in response to Elf3 silencing by siRNAs. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4409/8/8/858) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: ELF3/ESE-1 Antibody [NBP1-30873] - Methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - Human lung carcinoma. ESE1 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ELF3/ESE-1 Antibody [NBP1-30873] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: ELF3/ESE-1 Antibody [NBP1-30873] - NCI-N87 xenograft, using ESE1 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ELF3/ESE-1 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ESE1. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELF3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) -Reported in (PMID: 32439949).
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific publication (PMID: 31404945). Rat reactivity reported in scientific publication (PMID: 32439949).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ELF3/ESE-1 Antibody

• E74-like factor 3 (ets domain transcription factor, epithelial-specific )
• E74-like factor 3
• ELF3
• Epithelial-restricted with serine box
• epithelial-specific)
• Epithelium-restricted Ets protein ESX
• Epithelium-specific Ets transcription factor 1
• EPR1
• EPR-1
• ERT
• ESX
• ets domain transcription factor, serine box (epithelial-specific)
• ETS-related transcription factor Elf-3
• JEN
• serine box

Background

ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ELF3/ESE-1 Antibody (NBP1-30873)(2)

Publications using NBP1-30873

• Wang J, Shen X, Liu J et al. High glucose mediates NLRP3 inflammasome activation via upregulation of ELF3 expression Cell Death Dis 2020-05-21 [PMID: 32439949] (Chemotaxis, IHC-P, Rat)
• Sengez B, Aygun I, Shehwana H et al. The Transcription Factor Elf3 Is Essential for a Successful Mesenchymal to Epithelial Transition Cells 2019-08-09 [PMID: 31404945] (Chemotaxis, Mouse)

Bioinformatics

• Gene Symbol: ELF3