Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Gp, Gt, Ma, Pm, RbSpecies Glossary |
Applications | Flow, ICC/IF, IHC, IHC-Fr, IHC-P |
Clone | HM57 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Concentration | 1.0 mg/ml |
Immunogen | Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1. |
Specificity | NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications. |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Mouse |
Gene | CD79A |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: heat treated antigen retrieval using sodium citrate buffer pH 6.0 is recommended for this purpose. Although not tested this antibody may be useful in Western Blot. NB100-64347 has been used successfully in Immunofluorescence reported by a by a customer review. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | Phosphate buffered saline |
Preservative | 0.09% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein G purified |
Publications using NB100-64347 | Applications | Species |
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Skedsmo FS, Espenes A, Tranulis MA et al. Impaired NDRG1 functions in Schwann cells cause demyelinating neuropathy in a dog model of Charcot-Marie-Tooth type 4D Neuromuscular disorders : NMD Nov 26 2020 [PMID: 33334662] (IHC, Canine) | IHC | Canine |
Ali M, Abdel Hafez M, Mohamed M. The ameliorating effect of some immune-stimulants on bursal and tracheal lesions of broiler chickens challenged with ND virus JoBAZ Dec 1 2019 (IHC, Chicken) | IHC | Chicken |
Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||||||||||||||
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reviewed by:
Anonymous |
IHC-P | Rat | 02/17/2018 |
Summary
Comments
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reviewed by:
Anonymous |
IF | Other | 08/15/2012 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
Comments
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Secondary Antibodies |
Isotype Controls |
Alexa Fluor 350 | NB100-64347AF350 | |
Alexa Fluor 405 | NB100-64347AF405 | |
Alexa Fluor 488 | NB100-64347AF488 | |
Alexa Fluor 532 | NB100-64347AF532 | |
Alexa Fluor 594 | NB100-64347AF594 | |
Alexa Fluor 647 | NB100-64347AF647 | |
Alexa Fluor 700 | NB100-64347AF700 | |
Alexa Fluor 750 | NB100-64347AF750 | |
Allophycocyanin | NB100-64347APC | |
DyLight 350 | NB100-64347UV | |
DyLight 405 | NB100-64347V | |
DyLight 488 | NB100-64347G | |
DyLight 550 | NB100-64347R | |
DyLight 594 | NB100-64347DL594 | |
DyLight 650 | NB100-64347C | |
DyLight 680 | NB100-64347FR | |
DyLight 755 | NB100-64347IR | |
Janelia Fluor 549 | NB100-64347JF549 | |
Janelia Fluor 646 | NB100-64347JF646 | |
PE | NB100-64347PE | |
PerCP | NB100-64347PCP |
Diseases for CD79A Antibody (NB100-64347)Discover more about diseases related to CD79A Antibody (NB100-64347).
| Pathways for CD79A Antibody (NB100-64347)View related products by pathway.
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PTMs for CD79A Antibody (NB100-64347)Learn more about PTMs related to CD79A Antibody (NB100-64347).
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CD79b - A Signal Transduction Component of the B-cell Receptor The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway... Read full blog post. |
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Anonymous 02/17/2018 |
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Application: | IHC-P | |
Species: | Rat |
Anonymous 08/15/2012 |
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Application: | IF | |
Species: | Other |