CD79A Antibody (HM57)

Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer revies.

Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched isotype control NBP1-97005APC (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 0.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Ft, GP, Gt, Mk, Rb
• Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: HM57
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CD79A Antibody (HM57) Summary

• Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1.
• Specificity: NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD79A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
• Application Notes: For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: heat treated antigen retrieval using sodium citrate buffer pH 6.0 is recommended for this purpose. Although not tested this antibody may be useful in Western Blot. NB100-64347 has been used successfully in Immunofluorescence reported by a by a customer review.
• Positive Control:

  CD79A Lysate (NBL1-08969)

Reactivity Notes

Cross reacts with Red Deer, Fallow Deer and American Bison. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD79A Antibody (HM57)

• CD79A antigen (immunoglobulin-associated alpha)
• CD79a antigen
• CD79a molecule, immunoglobulin-associated alpha
• CD79A
• IGAB-cell antigen receptor complex-associated protein alpha chain
• ig-alpha
• MB-1 membrane glycoprotein
• MB1
• MB-1
• Membrane-bound immunoglobulin-associated protein
• Surface IgM-associated protein

Background

A disulfide-linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, is non-covalently associated with membrane bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for CD79A Antibody (NB100-64347) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Rat, Other.

reviewed by: Anonymous IHC-P Rat 02/17/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Spleen tissue
• Species: Rat
• Lot: 1601

Comments

• Comments: IHC performed on paraffin-embedded alcohol fixed tissue. Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4°C.

reviewed by: Anonymous IF Other 08/15/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Dog Lymphoma
• Species: Other
• Lot: 1106
• Comments: I thought the staining was decent, memebrane staining was seen on 20-30% of cells which is what is expected.

Blocking

• Blocking Details: Blcok in 5% Dog gammaglobulin and 2% Donkey serum for 10 minutes

Primary Anitbody

• Dilution Ratio: 1:50 dilution for 1hr at room temp in block buffer

Secondary Antibody

• Secondary Description: donkey anti mouse PE
• Secondary Manufacturer Cat#: Jackson immuno research 715-116-150
• Secondary Concentration: 1/2000 dilution

Details

• Detection Notes: Fluorescence based microscope (metasystems)
• Fixation Details: 2% PFA fixation for 20 min and 0.2% Triton X Perm in PBS for 5 minutes
• Wash Description: wash in PBS x3

Comments

• Comments: I thought the staining was decent, memebrane staining was seen on 20-30% of cells which is what is expected.

Bioinformatics

• Gene Symbol: CD79A
• Entrez:
  • 973(Human)
  • 12518(Mouse)
  • 681236(Rat)
• Uniprot:
  • P11912()