CD79A Antibody (HM57)

Images

 
Immunohistochemistry: CD79A Antibody (HM57) [NB100-64347] - Paraffin-embedded alcohol fixed Rat Spleen tissue (20x). Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4C. This image was submitted via customer ...read more
Flow Cytometry: CD79A Antibody (HM57) [NB100-64347] - Using the Allophycocyanin direct conjugate An intracellular stain was performed on Daudi cells with CD79A (HM 57) antibody NB100-64347APC (blue) and a matched ...read more
Immunohistochemistry-Paraffin: CD79A Antibody (HM57) [NB100-64347] - Embedded human tonsil.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq, Ft, GP, Gt, Mk, RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
HM57
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD79A Antibody (HM57) Summary

Immunogen
Synthetic peptide corresponding to 202-216 amino acid sequence of human mb-1.
Specificity
NB100-64347 recognizes an epitope within the cytoplasmic domain of CD79a. CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Clone HM57 has been reported to work in western blotting applications.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD79A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. For IHC: heat treated antigen retrieval using sodium citrate buffer pH 6.0 is recommended for this purpose. Although not tested this antibody may be useful in Western Blot. NB100-64347 has been used successfully in Immunofluorescence reported by a by a customer review.
Positive Control
CD79A Lysate (NBL1-08969)
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-64347 in the following applications:

Reactivity Notes

Cross reacts with Red Deer, Fallow Deer and American Bison. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD79A Antibody (HM57)

  • CD79A antigen (immunoglobulin-associated alpha)
  • CD79a antigen
  • CD79a molecule, immunoglobulin-associated alpha
  • CD79A
  • IGAB-cell antigen receptor complex-associated protein alpha chain
  • ig-alpha
  • MB-1 membrane glycoprotein
  • MB1
  • MB-1
  • Membrane-bound immunoglobulin-associated protein
  • Surface IgM-associated protein

Background

A disulfide-linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, is non-covalently associated with membrane bound immunoglobulins on B cells to constitute the B cell Ag receptor. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD79A Antibody (NB100-64347) (0)

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Reviews for CD79A Antibody (NB100-64347) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Rat, Other.

Reviews using NB100-64347:
Filter by Applications
IHC-P
(1)
IF
(1)
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Rat
(1)
Other
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD79A NB100-64347
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reviewed by:
Anonymous
IHC-P Rat 02/17/2018
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSpleen tissue
SpeciesRat
Lot1601

Comments

CommentsIHC performed on paraffin-embedded alcohol fixed tissue. Antigen retrieval pH6. Dilution: 1:500 and ON incubation at 4°C.
Immunofluorescence CD79A NB100-64347
Enlarge
reviewed by:
Anonymous
IF Other 08/15/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedDog Lymphoma
SpeciesOther
Lot1106
CommentsI thought the staining was decent, memebrane staining was seen on 20-30% of cells which is what is expected.

Blocking

Blocking DetailsBlcok in 5% Dog gammaglobulin and 2% Donkey serum for 10 minutes

Primary Anitbody

Dilution Ratio1:50 dilution for 1hr at room temp in block buffer

Secondary Antibody

Secondary Descriptiondonkey anti mouse PE
Secondary Manufacturer Cat#Jackson immuno research 715-116-150
Secondary Concentration1/2000 dilution

Details

Detection NotesFluorescence based microscope (metasystems)
Fixation Details2% PFA fixation for 20 min and 0.2% Triton X Perm in PBS for 5 minutes
Wash Descriptionwash in PBS x3

Comments

CommentsI thought the staining was decent, memebrane staining was seen on 20-30% of cells which is what is expected.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD79A Antibody (NB100-64347) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64347AF405
Alexa Fluor 488 Labeled NB100-64347AF488
Alexa Fluor 647 Labeled NB100-64347AF647
Alexa Fluor 700 Labeled NB100-64347AF700
Allophycocyanin Labeled NB100-64347APC
DyLight 350 Labeled NB100-64347UV
DyLight 405 Labeled NB100-64347V
DyLight 488 Labeled NB100-64347G
DyLight 680 Labeled NB100-64347FR
DyLight 755 Labeled NB100-64347IR
PE Labeled NB100-64347PE
PerCP Labeled NB100-64347PCP

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Blogs on CD79A.

CD79b - A Signal Transduction Component of the B-cell Receptor
The B-cell antigen receptor (BCR) is a complex multimeric aggregate that includes the following key noncovalently-bound components: antigen-specific surface immunoglobulin (Ig), CD79a (Ig-alpha), and CD79b (Ig-beta). BCR signaling is a pivotal pathway...  Read full blog post.

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Anonymous
02/17/2018
Application: IHC-P
Species: Rat

Anonymous
08/15/2012
Application: IF
Species: Other

Bioinformatics

Gene Symbol CD79A
Entrez
Uniprot