Ets-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human thymus tissue, mouse thymus tissue, and rat CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Ets-1 ...read more
Ets‑1 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human/Mouse/Rat Ets‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) at 5 µg/mL for 1 hour ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Ets-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Ets-1
Glu127-Val230
Accession # P14921
Specificity
Detects human, mouse, and rat Ets-1 in Western blots and detects recombinant human Ets-1 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ETS1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ets-1 Antibody [Unconjugated]

  • Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1
  • ets protein
  • Ets1
  • Ets-1
  • EWSR2
  • FLJ10768
  • p54
  • Protein C-Ets-1
  • v-ets avian erythroblastosis virus E2 oncogene homolog 1
  • v-ets avian erythroblastosis virus E26 oncogene homolog 1
  • v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
  • v-ets

Background

Ets-1 (E26 Transformation-Specific 1; also p54 and c-ets1) is a 52-54 kDa member of the ETS family of proteins. It is found in multiple cell types, and serves as a transcriptional regulator (generally activator) of multiple target genes, including prolactin, the transferrin receptor, and Cyclin E. By upregulating Cyclin E and CDK2 genes, it promotes cell-cycle progression. Ets‑1 forms complexes with both transcriptional activators (AP-1 and GHF-1) and repressors (MafB and Daxx). Human Ets‑1 is 441 amino acids (aa) in length. It contains one PNT domain (aa 51-136) that binds ERK2, and a DNA-binding ETS domain (aa 335-415). There are two SUMOylation sites, plus four utilized phosphorylation and acetylated lysine sites. At least four potential isoform variants are reported. One shows a deletion of aa 244-330 (termed isoform 1B), a second shows a deletion of aa 262-331, a third contains a deletion of aa 28-244, and a fourth possesses an 11 aa substitution for aa 262-441. Over aa 127-230, human Ets‑1 shares 95% aa identity with mouse Ets‑1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Blogs on Ets-1.

HIF-3 alpha: a versatile target with hypoxia dependent and independent functions
By: Subhash GangarHIF-3 alpha (hypoxia-inducible factor 3-alpha/ HIF3A) represents an isoform of HIF-alpha subunits which heterodimerize with stable beta subunit (HIF-beta) for the regulation of HIF target genes through binding to hypoxia respon...  Read full blog post.

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Bioinformatics

Gene Symbol ETS1
Uniprot