Blk Antibody (7A12)

Western Blot: Blk Antibody (7A12) [H00000640-M02] - Analysis of BLK expression in transfected 293T cell line by BLK monoclonal antibody (M02), clone 7A12.Lane 1: BLK transfected lysate (Predicted MW: 57.7 KDa).Lane 2: Non-transfected lysate.

Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in PC-12.

Western Blot: Blk Antibody (7A12) [H00000640-M02] - BLK monoclonal antibody (M02), clone 7A12. Analysis of BLK expression in Raw 264.7.

ELISA: Blk Antibody (7A12) [H00000640-M02] - Detection limit for recombinant GST tagged BLK is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, Flow
• Clone: 7A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Blk Antibody (7A12) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: BLK (AAH07371, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKG
• Localization: Nuclear
• Specificity: BLK - B lymphoid tyrosine kinase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BLK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA. FACS usage was reported in scientific literature.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Blk Antibody (7A12)

• B lymphocyte kinase
• B lymphoid tyrosine kinase
• BLK nonreceptor tyrosine kinase
• Blk
• EC 2.7.10.2
• MGC10442
• MODY11
• p55-Blk
• tyrosine-protein kinase Blk

Background

The gene encoding BLK is a member of the SRC family of protooncogenes and shows preferential expression in B-lymphoid cells. It is thought to function in a signal transductory pathway specific to this lineage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Blk Antibody (H00000640-M02)(4)

Publications using H00000640-M02

• Ni J, Li X, Tu X et al. Halofuginone ameliorates systemic lupus erythematosus by targeting Blk in myeloid-derived suppressor cells International immunopharmacology 2022-12-06 [PMID: 36493694] (WB, Mouse)
• Bernal-Quiros M, Wu YY, Alarcon-Riquelme ME et al. BANK1 and BLK Act through Phospholipase C Gamma 2 in B-Cell Signaling. PLoS One 2013-01-01 [PMID: 23555801] (Human)
• Simpfendorfer KR, Olsson LM, Manjarrez Orduno N et al. The autoimmunity-associated BLK haplotype exhibits cis-regulatory effects on mRNA and protein expression that are prominently observed in B cells early in development Hum Mol Genet 2012-06-20 [PMID: 22678060] (WB, FLOW, Human)
• Castillejo-Lopez C, Delgado-Vega AM, Wojcik J et al. Genetic and physical interaction of the B-cell systemic lupus erythematosus-associated genes BANK1 and BLK. Ann Rheum Dis. 2011-01-06 [PMID: 21978998]

Review for Blk Antibody (H00000640-M02) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Kim Simpfendorfer WB Human 05/16/2011

Summary

• Application: Western Blot
• Sample Tested: 100ng recombinant BLK, rLCK, rLYN, rFYN. 3ug Ramos whole cell lysate. 20ug Jurkat whole cell lysate.
• Species: Human
• Lot: 6342-7A12-00AaY6

FAQs for Blk Antibody (H00000640-M02). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of H00000640-M02 Lot E9231-7A12?
   • The concentration of Lot# Lot E9231-7A12 is 0.5mg/mL.

Bioinformatics

• Gene Symbol: BLK
• Entrez:
  • 640(Human)
• OMIM:
  • 191305(Human)
• Uniprot:
  • AAH07371(Human)