Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF


There are currently no images for Ephrin-A2 (8415-A2).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of PC‑3 human prostate cancer cells. The ED50 for this effect is 5-30 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Ephrin-A2 protein
Mouse Ephrin-A2
 Accession # P52801
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfied-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
52-61 kDa, reducing conditions

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF

  • Cek7-L
  • EFNA2
  • ELF-1
  • EPH-related receptor tyrosine kinase ligand 6
  • EphrinA2
  • Ephrin-A2
  • EPLG6HEK7 ligand
  • HEK7-L
  • HEK7-ligand
  • LERK6
  • ligand of eph-related kinase 6


Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4-7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7-9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up-regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1995) J. Biol. Chem. 270:3467.
  4. Cheng, H.-J., and J.G. Flanagan (1994) Cell 79:157.
  5. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
  6. Ohta, K. et al. (1997) Mech. Dev. 64:127.
  7. Wada, N. et al. (2003) Dev. Biol. 264:550.
  8. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
  9. Holmberg, J. et al. (2005) Genes Dev. 19:462.
  10. Nakamoto, M. et al. (1996) Cell 86:755.
  11. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
  12. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: Bind
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu, Mu, Rt
Applications: IHC, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Mu
Applications: Bind
Species: Hu
Applications: Bind
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: Flow, WB
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, WB
Species: Mu
Applications: IHC, WB
Species: Hu, Rt
Applications: CyTOF-ready, Flow, ICC, WB
Species: Mu
Applications: Bind
Species: Hu
Applications: ICC, IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Mu
Applications: Bind
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
Species: Hu, Mu
Applications: ChIP, IHC, IHC-P, IP, WB
Species: Hu
Applications: Flow, IHC, IHC-P, WB
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB

Publications for Ephrin-A2 (8415-A2) (0)

There are no publications for Ephrin-A2 (8415-A2).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Ephrin-A2 (8415-A2) (0)

There are no reviews for Ephrin-A2 (8415-A2). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Ephrin-A2 (8415-A2) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Ephrin-A2 Products

Bioinformatics Tool for Ephrin-A2 (8415-A2)

Discover related pathways, diseases and genes to Ephrin-A2 (8415-A2). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ephrin-A2 (8415-A2)

Discover more about diseases related to Ephrin-A2 (8415-A2).

Pathways for Ephrin-A2 (8415-A2)

View related products by pathway.

PTMs for Ephrin-A2 (8415-A2)

Learn more about PTMs related to Ephrin-A2 (8415-A2).

Blogs on Ephrin-A2

There are no specific blogs for Ephrin-A2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF and receive a gift card or discount.


Gene Symbol Efna2