eIF4A2 Antibody

Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.

Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Ca, Ch, Pm, Rt, Bv, Eq, Pm, Ma-Op, RM
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

eIF4A2 Antibody Summary

• Description: Novus Biologicals Rabbit eIF4A2 Antibody (NBP2-24529) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-eIF4A2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 50-100 of human eIF4A2 was used as the immunogen for the antibody.
• Specificity: The immunogen for this antibody has 100% homology to eIF4A1 and 89% homology to eIF4A3. Specificity to eIF4A2 has not been determined.
• Predicted Species: Rat (100%), Equine (100%), Monkey (100%), Opossum (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Simple Western 1:200
  • Western Blot 0.025-0.05 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF4A2 Antibody

• ATP-dependent RNA helicase eIF4A-2
• BM-010
• DDX2Beukaryotic translation initiation factor 4A, isoform 2
• EC 3.6.1
• EC 3.6.4.13
• EIF4A
• eIF-4A-II
• eIF4A-II
• EIF4F
• eukaryotic initiation factor 4A-II
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A2

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4A2 Antibody (NBP2-24529)(1)

Publication using NBP2-24529

• Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by promoting proteome remodeling on the mRNA cap bioRxiv 2020-07-14 [PMID: 34841428] (WB, ICC/IF, Human)

Review for eIF4A2 Antibody (NBP2-24529) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 02/18/2019

Summary

• Application: Western Blot
• Sample Tested: Mouse Embryonic Stem Cells
• Species: Mouse
• Lot: 080833758

Bioinformatics

• Gene Symbol: EIF4A2
• Uniprot:
  • Q14240(Human)