eIF4A2 Antibody

Images

 
Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ...read more
Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling ...read more
Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Ca, Ch, Pm, Rt, Bv, Eq, Pm, Ma-Op, RMSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

eIF4A2 Antibody Summary

Immunogen
A portion of amino acids 50-100 of human eIF4A2 was used as the immunogen for the antibody.
Specificity
The immunogen for this antibody has 100% homology to eIF4A1 and 89% homology to eIF4A3. Specificity to eIF4A2 has not been determined.
Predicted Species
Rat (100%), Equine (100%), Monkey (100%), Opossum (100%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4A2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Simple Western 1:200
  • Western Blot 0.025-0.05 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24529 in the following applications:

Publications
Read Publication using
NBP2-24529 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4A2 Antibody

  • ATP-dependent RNA helicase eIF4A-2
  • BM-010
  • DDX2Beukaryotic translation initiation factor 4A, isoform 2
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4A
  • eIF-4A-II
  • eIF4A-II
  • EIF4F
  • eukaryotic initiation factor 4A-II
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A2

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for eIF4A2 Antibody (NBP2-24529)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
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Review for eIF4A2 Antibody (NBP2-24529) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-24529:
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WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot eIF4A2 NBP2-24529
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5
reviewed by:
Verified Customer
WB Mouse 02/18/2019
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Summary

ApplicationWestern Blot
Sample TestedMouse Embryonic Stem Cells
SpeciesMouse
Lot080833758

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for eIF4A2 Antibody (NBP2-24529) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for eIF4A2 Antibody (NBP2-24529)

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Blogs on eIF4A2

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Recent Reviews

5
5
1
4
0
3
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2
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1
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Verified Customer
02/18/2019
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol EIF4A2
Uniprot