EEA1 Antibody - BSA Free

Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug each lane of HEK-293 cell extracts. Image from verified customer review.

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Paraffin-embedded colon carcinoma. Antibody at 1:500.

Western Blot: EEA1 Antibody [NBP1-30914] - Mouse brain. 5% SDS PAGE. Antibody at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug PC-12 whole cell lysate/extract, B. 30 ug Rat 2 whole cell lysate/extract. 5% SDS-PAGE. Antibody at 1:1000.

Western Blot: EEA1 Antibody [NBP1-30914] - 30 ug whole cell lysate/extracts B. Antibody at 1:5000.

Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - Methanol-fixed HeLa cells, using EEA1 (NBP1-30914) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled red.

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: EEA1 Antibody [NBP1-30914] - Mouse testis. EEA1 antibody [C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

EEA1 antibody [C3], C-term detects EEA1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: EEA1 protein stained by EEA1 antibody [C3], C-term (NBP1-30914) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EEA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EEA1 Antibody - BSA Free (NBP1-30914) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-EEA1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EEA1. The exact sequence is proprietary.
• Localization: Cytoplasm, Early endosome membrane
• Marker: Early Endosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEA1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunomicroscopy Reported in scientific literature (PMID: 27795427)
  • Western Blot 1:500-1:10000
• Theoretical MW: 162 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for EEA1 Antibody - BSA Free

• early endosome antigen 1
• early endosome antigen 1, 162kD
• early endosome-associated protein
• EEA1
• Endosome-associated protein p162
• MST105
• MSTP105
• ZFYVE2
• ZFYVE2MST105
• Zinc finger FYVE domain-containing protein 2

Background

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for EEA1 Antibody (NBP1-30914)(9)

Publications using NBP1-30914

• Murakami R, Chiba Y, Takebayashi G et al. SPINT1 Expressed in Epithelial Cells of Choroid Plexus in Human and Mouse Brains. International Journal of Molecular Sciences 2025-06-13 [PMID: 40507942]
• Das S, Parray H, Chiranjivi A et al. Kennedy epitope (KE)-dependent retrograde transport of efficiently cleaved HIV-1 envelopes (Envs) and its effect on Env cell surface expression and viral particle formation Research Square 2022-11-10 (Western Blot, Human)
• Momei Zhou, Benjamin Vollmer, Emily Machala, Muyuan Chen, Kay Grünewald, Ann M Arvin, Wah Chiu, Stefan L Oliver Targeted mutagenesis of the herpesvirus fusogen central helix captures transition states. Nature communications 2023-12-04 [PMID: 38042814]
• Supratik Das, Hilal Ahmad Parray, Adarsh Kumar Chiranjivi, Prince Kumar, Abhishek Goswami, Manish Bansal, Deepak Kumar Rathore, Rajesh Kumar, Sweety Samal Kennedy Epitope (KE)-dependent Retrograde Transport of Efficiently Cleaved HIV-1 Envelopes (Envs) and its Effect on Env Cell Surface Expression and Viral Particle Formation. The protein journal 2023-10-04 [PMID: 37794304]
• Murakami R, Chiba Y, Nishi N et al. Immunoreactivity of receptor and transporters for lactate located in astrocytes and epithelial cells of choroid plexus of human brain Neurosci Lett 2020-11-16 [PMID: 33212210] (ICC/IF, Human)
  
  Details:
  Immunofluorescence analysis and immunohistochemical analysis of human brain samples. Western blot analysis performed in HeLa and HT-29.
• Yang E, Arvin AM, Oliver SL. The Glycoprotein B Cytoplasmic Domain Lysine Cluster Is Critical for Varicella Zoster Virus Cell-Cell Fusion Regulation and Infection. J. Virol. 2016-10-26 [PMID: 27795427] (IM)
• Yang E, Arvin AM, Oliver SL. The cytoplasmic domain of varicella-zoster virus glycoprotein h regulates syncytia formation and skin pathogenesis. PLoS Pathog. 2014-05-01 [PMID: 24874654] (ICC/IF, Human)
  
  Details:
  Human melanoma cells transiently expressing gH[WT] or gH mutants with gL at 24 hours post transfection were immunostained for early endosome antigen (EEA1) with anti-EEA1 antibody - immunostaining appearing as punctae in the cytoplasm (Figure 4). Human melanoma cells at 24 (Uninfected) or infection with pOka-gH[?834-841], pOka-gH[TL], pOka-gH[Y835A], pOka-gH[Y835F], pOka-gH[V5], pOka-gH[834StopV5], or pOka viruses were also stained for EEA1 (Figure 8).
• Oliver SL, Brady JJ, Sommer MH et al. An immunoreceptor tyrosine-based inhibition motif in varicella-zoster virus glycoprotein B regulates cell fusion and skin pathogenesis. Proc Natl Acad Sci U S A 2013-01-29 [PMID: 23322733] (ICC/IF, Human)
• Chen YT, Tsai MS, Yang TL et al. R26R-GR: a Cre-activable dual fluorescent protein reporter mouse. PLoS One 2012-01-01 [PMID: 23049968] (ICC/IF, Human)

Review for EEA1 Antibody (NBP1-30914) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Luiz Alexandre Magno WB Human 05/05/2019

Summary

• Application: Western Blot
• Sample Tested: HEK 293 whole cell lysate
• Species: Human
• Lot: 39959

Comments

• Comments: Antibody concentration: 1:1,000
  Incubation time/temp: overnight at 4C (shaking)

Bioinformatics

• Gene Symbol: EEA1
• Entrez:
  • 8411(Human)
• Uniprot:
  • Q15075(Human)