SFRS5 Antibody

Images

 
Western Blot: SFRS5 Antibody [H00006430-B01P] - Analysis of SFRS5 expression in human liver.
Immunocytochemistry/ Immunofluorescence: SFRS5 Antibody [H00006430-B01P] - Analysis of purified antibody to SFRS5 on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: SFRS5 Antibody [H00006430-B01P] - Analysis of purified antibody to SFRS5 on formalin-fixed paraffin-embedded human ovary. (antibody concentration 1.5 ug/ml)
Western Blot: SFRS5 Antibody [H00006430-B01P] - Analysis of SFRS5 expression in transfected 293T cell line by SFRS5 polyclonal antibody. Lane 1: SFRS5 transfected lysate(30.03 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SFRS5 Antibody Summary

Immunogen
SFRS5 (AAH18823, 1 a.a. - 272 a.a.) full-length human protein. MSGCRVFIGRLNPAAREKGVERFFKGYGRIRDIDLKRGFGFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRFSSRRPRNDRRNAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKNAIEKLSGKEINGRKIKLIEGSKRHSRSRSRSRSRTRSSSRSRSRSRSRSRKSYSRSRSRSRSRSRSKSRSVSRSPVPEKSQKRGSSSRSKSPASVDRQRSRSRSRSRSVDSGN
Specificity
SFRS5 - splicing factor, arginine/serine-rich 5,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
SRSF5
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactivity against tissue lystae and transfected lysate for WB. It has been used for IF, IHC-P, and ELISA.
Reviewed Applications
Read 1 Review rated 3
using
H00006430-B01P in the following applications:

Publications
Read Publication using H00006430-B01P.

Reactivity Notes

Rat reactivity reported from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SFRS5 Antibody

  • Delayed-early protein HRS
  • HRSSFRS5
  • serine/arginine-rich splicing factor 5
  • Splicing factor, arginine/serine-rich 5Pre-mRNA-splicing factor SRP40
  • SRP40SR splicing factor 5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SFRS5 Antibody (H00006430-B01P)(1)

Review for SFRS5 Antibody (H00006430-B01P) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IP.

Reviews using H00006430-B01P:
Filter by Applications
IP
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IP Human 01/28/2009
View

Summary

ApplicationImmunoprecipitation
Sample TestedHEK 293T cells
SpeciesHuman
Lot08256WULz
CommentsThis antibody did not conjugate well to the beads for this experiment but may work better with a larger volume. As a western antibody it recognized the immunoprecipitated protein in a single specific band but detection of endogenous protein was weak. Lysate Amount: 0.25mg, Protein Capture: Protein G

Primary Anitbody

Dilution RatioIncubation Dilution Buffer: 0.1M HEPES, Dilution Ratio: 100ng/ml, Incubation Time: overnight, Incubation Temp: 4 degrees Celsius

Comments

CommentsThis antibody did not conjugate well to the beads for this experiment but may work better with a larger volume. As a western antibody it recognized the immunoprecipitated protein in a single specific band but detection of endogenous protein was weak. Lysate Amount: 0.25mg, Protein Capture: Protein G

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SFRS5 Antibody (H00006430-B01P) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on SFRS5

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Recent Reviews

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Anonymous
01/28/2009
Application: IP
Species: Human

Bioinformatics

Gene Symbol SRSF5
Entrez
Uniprot