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Rab11A Antibody

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Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue
Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: ...read more
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human colon shows moderate positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Rab11A Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RRENDMSPSNNVVPIHVPPTTENKPKVQ
Specificity
Specificity of human Rab11A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB11A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control
Rab11A Knockout HeLa Cell Lysate
Control Peptide
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-49320 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rab11A Antibody

  • MGC1490
  • RAB 11A, member oncogene family
  • RAB11
  • Rab-11
  • Rab11A
  • RAB11A, member RAS oncogene family
  • YL8
  • YL8ras-related protein Rab-11A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x.
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Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)
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Immunohistochemistry-Paraffin: Rab4 Antibody [NB100-74519] - Immunohistochemistry was performed on normal deparaffinized human Stomach tissue.
Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NB100-74519] - in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAB4 polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
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HIV-1 Rev binding
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Immunocytochemistry/Immunofluorescence: HIV-1 Rev binding protein (HRB) Antibody [NBP1-91991] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: HIV-1 Rev binding protein (HRB) Antibody [NBP1-91991] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
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Immunocytochemistry/Immunofluorescence: RAB7B Antibody (3B3) [H00338382-M01] - Mouse primary cells stained with RAB7B antibody. Image from verified customer review.
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Immunocytochemistry/Immunofluorescence: Rab7a Antibody [NBP1-87174] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rab7a Antibody [NBP1-87174] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.
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Western Blot: RAB11FIP5 Antibody [NBP1-81855] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Immunohistochemistry-Paraffin: RAB11FIP5 Antibody [NBP1-81855] - Staining in human testis and endometrium tissues using anti-RAB11FIP5 antibody. Corresponding RAB11FIP5 RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: ARF6 Antibody (JF101-2) [NBP2-67165] - Staining ARF6 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemistry-Frozen: RAB11B Antibody [NBP2-15085] - Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.
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Immunohistochemistry-Paraffin: RAB43 Antibody (5G4) [H00339122-M01] - Analysis of monoclonal antibody to RAB43 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
ELISA: RAB43 Antibody (5G4) [H00339122-M01] - Detection limit for recombinant GST tagged RAB43 is approximately 0.1ng/ml as a capture antibody.
(1 Review)
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Western Blot: RAB6A Antibody [NBP1-33110] - A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 12 % SDS-PAGE RAB6A antibody dilution: 1:1000
Immunocytochemistry/Immunofluorescence: RAB6A Antibody [NBP1-33110] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB6A protein stained by RAB6A antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
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Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in glandular cells.
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IKK gamma
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In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye.
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Western Blot: Rab25 Antibody (3F12) [NBP1-51599] - Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunocytochemistry/Immunofluorescence: Rab25 Antibody (3F12) [NBP1-51599] - Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) [NBP1-51599] - Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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NBP1-57009
Western Blot: RAB11FIP2 Antibody [NBP1-57009] - COLO205 cells lysate, concentration 0.2-1 ug/ml.
(1 Publication)
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, GP, Rb
Applications: WB, ICC/IF
RAB11FIP3 Antibody
NBP2-20034
Western Blot: RAB11FIP3 Antibody [NBP2-20034] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: RAB11FIP3 Antibody [NBP2-20034] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Species: Hu
Applications: WB, IHC, IHC-P
Rab11A Antibody
NBP2-49320
Rab11A Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Rab11A Antibody (NBP2-49320) (0)

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Reviews for Rab11A Antibody (NBP2-49320) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-49320:
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Images Ratings Applications Species Date Details
Immunocytochemistry Rab11A NBP2-49320
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reviewed by:
Scott Messenger
 ICC Human 03/28/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedMDA MB 231 cells
SpeciesHuman

Comments

CommentsMDA-MB-231 cells using antibody 1/100
Western Blot Rab11A NBP2-49320
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reviewed by:
Anonymous
 WB Human 01/20/2017
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman

Comments

Commentsstrongest band at ~25 kDa non-specific bands found at >50kDa not lost with shRNA knockdown. Blot is dirty and could be cleaned up with better experimental technique.

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WB Video Protocol
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FAQs for Rab11A Antibody (NBP2-49320) (0)

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Control Lysate(s)

Rab11A Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Immunocytochemistry Rab11A NBP2-49320
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 Scott Messenger
03/28/2017
Application: ICC
Species: Human
read this review
Western Blot Rab11A NBP2-49320
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 Anonymous
01/20/2017
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol RAB11A