Rab11A Antibody

Images

 
Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: ...read more
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human stomach shows moderate cytoplasmic positivity in parietal cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human colon shows moderate positivity in glandular cells.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Rab11A Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RRENDMSPSNNVVPIHVPPTTENKPKVQ
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB11A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence validated from a verified customer review.
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Rab11A Recombinant Protein Antigen (NBP2-49320PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-49320 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rab11A Antibody

  • MGC1490
  • RAB 11A, member oncogene family
  • RAB11
  • Rab-11
  • Rab11A
  • RAB11A, member RAS oncogene family
  • YL8
  • YL8ras-related protein Rab-11A

Background

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC

Publications for Rab11A Antibody (NBP2-49320) (0)

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Reviews for Rab11A Antibody (NBP2-49320) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-49320:
Filter by Applications
ICC
(1)
WB
(1)
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Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Immunocytochemistry Rab11A NBP2-49320
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4
reviewed by:
Scott Messenger
ICC Human 03/28/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedMDA MB 231 cells
SpeciesHuman

Comments

CommentsMDA-MB-231 cells using antibody 1/100
Western Blot Rab11A NBP2-49320
Enlarge
4
reviewed by:
Verified Customer
WB Human 01/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman

Comments

Commentsstrongest band at ~25 kDa non-specific bands found at >50kDa not lost with shRNA knockdown. Blot is dirty and could be cleaned up with better experimental technique.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rab11A Antibody (NBP2-49320) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
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Scott Messenger
03/28/2017
Application: ICC
Species: Human

Verified Customer
01/20/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol RAB11A