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Rab11A Antibody

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Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue
Western Blot: Rab11A Antibody [NBP2-49320] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: ...read more
Immunocytochemistry/ Immunofluorescence: Rab11A Antibody [NBP2-49320] - MDA MB 231 Cells, image courtesy of customer.
Immunohistochemistry-Paraffin: Rab11A Antibody [NBP2-49320] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Rab11A Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: RRENDMSPSNNVVPIHVPPTTENKPKVQ
Specificity
Specificity of human Rab11A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RAB11A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Rab11A Recombinant Protein Antigen (NBP2-49320PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-49320 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rab11A Antibody

  • MGC1490
  • RAB 11A, member oncogene family
  • RAB11
  • Rab-11
  • Rab11A
  • RAB11A, member RAS oncogene family
  • YL8
  • YL8ras-related protein Rab-11A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x.
Immunohistochemistry-Paraffin: Rab5a Antibody [NB120-13253] - analysis using Rabbit Anti-Rab5 Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative Solution. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.
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Applications: WB, ICC/IF, IHC, IHC-P, IP
Transferrin
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Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)
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Immunohistochemistry-Paraffin: Rab4 Antibody [NB100-74519] - Immunohistochemistry was performed on normal deparaffinized human Stomach tissue.
Immunocytochemistry/Immunofluorescence: Rab4 Antibody [NB100-74519] - in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAB4 polyclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Species: Hu, Mu, Rt, Ca, Pm
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HIV-1 Rev binding
protein (HRB) Antibod ...
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Western Blot: HIV-1 Rev binding protein (HRB) Antibody [NBP1-91991] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: HIV-1 Rev binding protein (HRB) Antibody [NBP1-91991] - Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Immunohistochemistry-Paraffin: HIV-1 Rev binding protein (HRB) Antibody [NBP1-91991] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: RAB7B Antibody (3B3) [H00338382-M01] - RAB7B monoclonal antibody (M01), clone 3B3. Analysis of RAB7B expression in human kidney.
Immunocytochemistry/Immunofluorescence: RAB7B Antibody (3B3) [H00338382-M01] - Mouse primary cells stained with RAB7B antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: RAB7B Antibody (3B3) [H00338382-M01] - Monoclonal antibody to RAB7B ( Cat # H00338382-M01 ) on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
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Western Blot: RAB7A Antibody [NBP1-05048] - Detection of RAB7 in NIH/3T3 cell lysates
Immunocytochemistry/Immunofluorescence: RAB7A Antibody [NBP1-05048] - IF staining of Rab7a in hepatocytes. Image from verified customer review.
Immunocytochemistry/Immunofluorescence: RAB7A Antibody [NBP1-05048] - Rab-7a antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
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Species: Hu, Mu, Rt, Bv, Ca, Sh
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H00004218-M02
Western Blot: RAB8A Antibody (3G1) [H00004218-M02] - RAB8A monoclonal antibody (M02), clone 3G1 Analysis of RAB8A expression in Hela S3 NE.
Immunocytochemistry/Immunofluorescence: RAB8A Antibody (3G1) [H00004218-M02] - Neuromast hair cells of zebrafish embryos stained with RAB8A antibody. Donkey anti-mouse Alexa Fluor 594 secondary antibody was used. Image from verified customer.
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Western Blot: RAB11FIP5 Antibody [NBP1-81855] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: FIP1/RCP Antibody (1A12B9D8) [NBP2-52546] - Analysis using RAB11FIP1 mAb against human RAB11FIP1 (AA: 130-271) recombinant protein. (Expected MW is 41.7 kDa)
Immunohistochemistry-Paraffin: FIP1/RCP Antibody (1A12B9D8) [NBP2-52546] - Analysis of paraffin-embedded bladder cancer tissues using RAB11FIP1 mouse mAb with DAB staining.
Flow Cytometry: FIP1/RCP Antibody (1A12B9D8) [NBP2-52546] - Analysis of Raji cells using RAB11FIP1 mouse mAb (green) and negative control (red).
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
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Western Blot: EEA1 Antibody [NBP1-30914] - A. 30 ug HepG2 whole cell lysate/extract B. 30 ug A375 whole cell lysate/extract 5 % SDS-PAGE EEA1 antibody [C3], C-termdilution: 1:5000
Immunocytochemistry/Immunofluorescence: EEA1 Antibody [NBP1-30914] - DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 protein stained by EEA1 antibody [C3], C-term diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [886] (NBP2-43561) diluted at 1:500. Blue: Fluoroshield with DAPI.
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Western Blot: ARF6 Antibody [NBP2-41263] - Western blot analysis of ARF6 in rat liver tissue lysate with ARF6 antibody at 1 ug/ml.
Immunohistochemistry: ARF6 Antibody [NBP2-41263] - ARF6 in human kidney tissue with ARF6 antibody at 5 ug/mL.
Immunofluorescence: ARF6 Antibody [NBP2-41263] - ARF6 in human kidney tissue with ARF6 antibody at 20 ug/mL.
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
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Immunocytochemistry/Immunofluorescence: RAB11B Antibody [NBP2-15085] - HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min. Green.
Immunohistochemistry-Frozen: RAB11B Antibody [NBP2-15085] - Frozen-sectioned adult mouse cerebellum. Green: RAB11B stained by RAB11B antibody [N1C3] diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
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Western Blot: RAB43 Antibody (5G4) [H00339122-M01] - RAB43 monoclonal antibody (M01), clone 5G4 Analysis of RAB43 expression in HepG2.
Immunohistochemistry-Paraffin: RAB43 Antibody (5G4) [H00339122-M01] - Analysis of monoclonal antibody to RAB43 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
ELISA: RAB43 Antibody (5G4) [H00339122-M01] - Detection limit for recombinant GST tagged RAB43 is approximately 0.1ng/ml as a capture antibody.
(1 Review)
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Applications: WB, ELISA, IHC, IHC-P
RAB6A Antibody
NBP1-33110
Western Blot: RAB6A Antibody [NBP1-33110] - Wild-type (WT) and RAB6A knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB6A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: RAB6A Antibody [NBP1-33110] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAB6A protein stained by RAB6A antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: RAB6A Antibody [NBP1-33110] - Paraffin-embedded MDA-MB-468 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Myosin VB Antibody
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Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining in human small intestine and skeletal muscle tissues. Corresponding MYO5B RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Myosin VB Antibody [NBP1-87746] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in glandular cells.
(3 Publications)
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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
IKK gamma
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NBP2-26504
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In vivo assay: IKK gamma Inhibitor Peptide Set [NBP2-26504] - NBD peptide blocks constitutive NF-kB activation in human multiple myeloma cells. U266 cells were treated with 100 uM of control (A & B) or NBD peptide (C & D) for 12 hr, cytospun, plated on glass slides, air dried for 1 hr at room temperature and fixed with cold acetone. Slides were blocked with 5% normal goat serum for 1 hr and then incubated with rabbit polyclonal anti-human p65 antibody (A & C) followed by Ig-Alexa 594 second step. In control peptide treated cells, p65 translocates to nucleus (A), whereas NBD peptide prevents translocation of p65 into the nucleus (C). B & D: Nuclear staining with DNA binding dye.
IKK gamma Inhibitor Peptide Set [NBP2-26504] - The IKK complex consists of IKKalpha + IKKbeta+ NEMO. NEMO (IKKgamma/NF-kB Essential MOdulator) is a scaffold protein required for the kinase activity of IKKalpha + IKKbeta. NBD (NEMO Binding Domain) inhibits formation of the IKK complex (IKKalpha + IKKbeta+ NEMO) by binding to NEMO and blocking IKK complex formation. In this regard, NBD is considered to be an 'IKKalpha and IKKBeta decoy' Blocking IKK complex formation suppresses downstream events in the NF-kB signaling pathway that rely on the IKK complex including phosphorylation of IkBalpha, and subsequence IkBalpha ubiquination, degradation and release of NF-kB. Hence, NF-kB is retained in the cytoplasm in its inactive state.
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Western Blot: Rab25 Antibody (3F12) [NBP1-51599] - Western blot analysis using Rab25 mouse mAb against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.
Immunocytochemistry/Immunofluorescence: Rab25 Antibody (3F12) [NBP1-51599] - Immunofluorescence analysis of A549 cells using RAB25 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Rab25 Antibody (3F12) [NBP1-51599] - Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 mouse mAb with DAB staining.
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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NBP1-82949
Immunocytochemistry/Immunofluorescence: RAB11FIP2 Antibody [NBP1-82949] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunohistochemistry-Paraffin: RAB11FIP2 Antibody [NBP1-82949] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
RAB11FIP3 Antibody
NBP1-83998
Immunohistochemistry-Paraffin: RAB11FIP3 Antibody [NBP1-83998] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
(1 Publication)
Species: Hu
Applications: IHC, IHC-P
Rab11A Antibody
NBP2-49320
Rab11A Antibody
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for Rab11A Antibody (NBP2-49320) (0)

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Reviews for Rab11A Antibody (NBP2-49320) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-49320:
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Images Ratings Applications Species Date Details
Immunocytochemistry Rab11A NBP2-49320
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reviewed by:
Scott Messenger
 ICC Human 03/28/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedMDA MB 231 cells
SpeciesHuman

Comments

CommentsMDA-MB-231 cells using antibody 1/100
Western Blot Rab11A NBP2-49320
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reviewed by:
Anonymous
 WB Human 01/20/2017
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate
SpeciesHuman

Comments

Commentsstrongest band at ~25 kDa non-specific bands found at >50kDa not lost with shRNA knockdown. Blot is dirty and could be cleaned up with better experimental technique.

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Recent Reviews

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Immunocytochemistry Rab11A NBP2-49320
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 Scott Messenger
03/28/2017
Application: ICC
Species: Human
read this review
Western Blot Rab11A NBP2-49320
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 Anonymous
01/20/2017
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol RAB11A
Entrez