EEA1 Antibody

Western Blot: EEA1 Antibody [NBP1-05962] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963.

• Reactivity: Hu, Mu, Sh
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EEA1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human early endosome antigen 1 using the numbering given in entry NP_003557.2 (GeneID 8411).
• Marker: Early Endosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: ICC/IF reactivity reported in (PMID: 30016419).

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 30016419).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EEA1 Antibody

• early endosome antigen 1
• early endosome antigen 1, 162kD
• early endosome-associated protein
• EEA1
• Endosome-associated protein p162
• MST105
• MSTP105
• ZFYVE2
• ZFYVE2MST105
• Zinc finger FYVE domain-containing protein 2

Background

Early endosomal autoantigen 1 (EEA1) bears an FYVE (Fab1p-YOPB-Vps27p-EEA1) domain which is an approximately 80 amino acid double-zinc binding motif that binds to PtdIns(3)P (phosphatidylinositol 3-phosphate). The FYVE domain is found in proteins involved in intracellular signaling, membrane trafficking, and cytoskeletal rearrangement. EEA1 binds to early endosomal membranes that contain PtdIns(3)P and plays a role in the docking and fusion of early endosomes. Alternative names for EEA1 include endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2, and ZFYVE2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EEA1 Antibody (NBP1-05962)(7)

Publications using NBP1-05962

• Yao K, Wang Z, Peng C et Al. HIV protease inhibitor saquinavir inhibits toll-like receptor 4 activation by targeting receptor dimerization Immunopharmacol Immunotoxicol 2023-12-06 [PMID: 37485845]
• Weems PW, Coolen LM, Hileman SM et al. EVIDENCE THAT DYNORPHIN ACTS UPON kNDy AND GnRH NEURONS DURING GnRH PULSE TERMINATION IN THE EWE. Endocrinology. 2018-07-16 [PMID: 30016419] (ICC/IF, Sheep)
• Nakayama A, Nakayama M, Turner CJ et al. Ephrin-B2 controls PDGFR-beta internalization and signaling. Genes Dev 2013-12-01 [PMID: 24298057] (WB, Mouse)
• Fahrer J, Rausch J, Barth H. A Cell-Permeable Fusion Protein Based on Clostridium botulinum C2 Toxin for Delivery of p53 Tumorsuppressor into Cancer Cells. PLoS One. 2013-09-05 [PMID: 24039769] (Human)
• Uchiyama K, Muramatsu N, Yano M et al. Prions disturb post-Golgi trafficking of membrane proteins. Nat Commun 2013-01-01 [PMID: 23673631] (ICC/IF, Mouse)
• Peng C, Deng G, Wang Y et al. HIV protease inhibitor Saquinavir inhibits toll-like receptor 4 activation by targeting receptor dimerization bioRxiv 2018-05-23
• Farmer T, Xie S, Naslavsky N et al. Defining the protein and lipid constituents of tubular recycling endosomes The Journal of biological chemistry 2020-12-17 [PMID: 33334886]

Bioinformatics

• Gene Symbol: EEA1
• Entrez:
  • 8411(Human)
  • 216238(Mouse)
• Uniprot:
  • Q15075(Human)