DUOX2 Antibody - BSA Free

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Genetic Strategies: Knockdown Validated: DUOX2 Antibody [NB110-61576] - Expression profile and silencing of NADPH-oxidases in mesenchymal cells.3T3 fibroblasts were transiently transfected by siRNAs to Duox1 or ...read more
Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.
Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more
Western Blot: DUOX2 Antibody [NB110-61576] - Generation and validation of NOX4 knockout (KO) HeLa cell lines. NOX4 knockout did not influence NOX1, NOX2, NOX3, NOX5, DUOX1 and DUOX2 levels. Protein levels of NOX1, NOX2, ...read more
Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

DUOX2 Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to an internal portion of human DUOX2 (within residues 400-500). [Swiss-Prot# Q9NRD8]
Localization
Apical cell membrane; Multi-pass membrane protein. Note: Localizes to the apical membrane of epithelial cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DUOX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 2 ug/ml
Application Notes
This DUOX2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~150 kDa, and in Immunocytochemistry/Immunofluorescence membrane staining was seen in A431 cells. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24492313)
Publications
Read Publications using
NB110-61576 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DUOX2 Antibody - BSA Free

  • dual oxidase 2
  • dual oxidase-like domains 2
  • EC 1.11.1.-
  • EC 1.6.3.1
  • flavoprotein NADPH oxidase
  • Large NOX 2
  • LNOX2P138(TOX)
  • Long NOX 2
  • NADH/NADPH thyroid oxidase p138-tox
  • NADPH oxidase/peroxidase DUOX2
  • NADPH thyroid oxidase 2
  • nicotinamide adenine dinucleotide phosphate oxidase
  • NOXEF2
  • p138 thyroid oxidase
  • P138-TOX
  • THOX2TDH6
  • Thyroid oxidase 2

Background

DUOX2 (dual oxidase 2), a multi-pass membrane protein localized to the apical membrane of epithelial cells, belongs to NADPH oxidase (NOX) family which is known to reduce molecular oxygen with NADPH as electron donor and unlike most of NOX-family members that produce superoxide anions (O2.-) as primary product, DUOX directly produce H2O2. DUOX2 and DUOX 1 require specific maturation factors, DUOXA1 and DUOXA2, to be functionally expressed at the apical follicular membrane, the site for hormonogenesis. DUOX2/DUOX 1 are major components of H2O2-generating system and generate H2O2 which is required for activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. DUOX2 plays a role in thyroid hormones synthesis and LPO-mediated antimicrobial defense mucosal surface. It has its own peroxidase activity through its N-terminal peroxidase-like domain which is inhibited by aminobenzohydrazide and the NADPH oxidase activity is calcium-dependent. DUOX2 is expressed in thyrocytes, intestine, kidney, liver, lung, pancreas, prostate, salivary glands, testis etc. and is induced by forskolin, thyrotropin and the Th1-specific cytokine IFNG/IFN-gamma. Defects in DUOX2 have been linked to thyroid dyshormonogenesis 6 (TDH6) characterized by defective conversion of accumulated iodide to organically bound iodine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DUOX2 Antibody (NB110-61576)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(5)
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Filter By Species
Human
(3)
Mouse
(3)
Porcine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-61576 Applications Species
Dyikanov D, Vasiluev P, Rysenkova K et al. Optimization of CRISPR/Cas9 technology to knock-out genes of interest in aneuploid cell lines Tissue Eng Part C Methods Feb 12 2019 [PMID: 30747044] (WB) WB
Jafari N, Kim H, Park R et al. CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells PLoS ONE Jan 18 2017 [PMID: 28099519] (WB, Human) WB Human
Tyurin-Kuzmin PA, Zhdanovskaya ND, Sukhova AA et al. Nox4 and Duox1/2 Mediate Redox Activation of Mesenchymal Cell Migration by PDGF. PLoS ONE. 2016 Apr 26 [PMID: 27110716] (WB, Mouse) WB Mouse
Wakatsuki S, Furuno A, Ohshima M, Araki T. Oxidative stress-dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration. J. Cell Biol. 2015 Nov 23 [PMID: 26572622] (ICC/IF, Mouse) ICC/IF Mouse
Macfie TS, Poulsom R, Parker A et al. DUOX2 and DUOXA2 Form the Predominant Enzyme System Capable of Producing the Reactive Oxygen Species H2O2 in Active Ulcerative Colitis and are Modulated by 5-Aminosalicylic Acid. Inflamm. Bowel Dis. 2014 Feb 14 [PMID: 24492313] (IHC-P, Human) IHC-P Human
Morand, S et al. Effect of iodide on nicotinamide adenine dinucleotide phosphate oxidase activity and Duox2 protein expression in isolated porcine thyroid follicles. Endocrinology;144(4):1241-8. 2003 Apr. [PMID: 12639906] (WB, Porcine) WB Porcine
Liang T, Zhang N, Ju H et al. Telocytes Reduce Oxidative Stress by Downregulating DUOX2 Expression in Inflamed Lungs of Mice Research Square Feb 9 2021 (WB, Human, Mouse) WB Human, Mouse

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DUOX2 Antibody (NB110-61576). (Showing 1 - 2 of 2 FAQs).

  1. Hi, I am looking for an antibody to human DUOX2 that will work on human formalin fixed paraffin embedded sections. I notice from your website that you have several recommended for WB and wondered if you have any experience of their use in other areas?
    • At this time we do not have an antibody for DUOX2 that works in IHC paraffin embedded sections. If you choose to try one in this application we have our Innovator's Reward program where we reward our customers for trying antibodies in previously untested applications and species.
  2. Can you please let me know the homology of the immunogen peptide with mouse, for NB110-61576? Here is a link to the mouse DUOX2 protein information: ncbi
    • The homology is 84.6%.

Secondary Antibodies

 

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Array NB110-61576

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Blogs on DUOX2.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

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Bioinformatics

Gene Symbol DUOX2