DUOX2 Antibody

Images

 
Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.
Immunocytochemistry/ Immunofluorescence: DUOX2 Antibody [NB110-61576] - DUOX2 antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ICC/IF, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.2 mg/ml

DUOX2 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human DUOX2 (within residues 400-500). [Swiss-Prot# Q9NRD8]
Localization
Apical cell membrane; Multi-pass membrane protein. Note: Localizes to the apical membrane of epithelial cells.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DUOX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry-Paraffin
Application Notes
This DUOX2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot. In Western blot a band is observed at ~150 kDa, and in Immunocytochemistry/Immunofluorescence membrane staining was seen in A431 cells. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24492313)
Publications
Read Publications using
NB110-61576 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DUOX2 Antibody

  • dual oxidase 2
  • dual oxidase-like domains 2
  • EC 1.11.1.-
  • EC 1.6.3.1
  • flavoprotein NADPH oxidase
  • Large NOX 2
  • LNOX2P138(TOX)
  • Long NOX 2
  • NADH/NADPH thyroid oxidase p138-tox
  • NADPH oxidase/peroxidase DUOX2
  • NADPH thyroid oxidase 2
  • nicotinamide adenine dinucleotide phosphate oxidase
  • NOXEF2
  • p138 thyroid oxidase
  • P138-TOX
  • THOX2TDH6
  • Thyroid oxidase 2

Background

DUOX2 (dual oxidase 2), a multi-pass membrane protein localized to the apical membrane of epithelial cells, belongs to NADPH oxidase (NOX) family which is known to reduce molecular oxygen with NADPH as electron donor and unlike most of NOX-family members that produce superoxide anions (O2.-) as primary product, DUOX directly produce H2O2. DUOX2 and DUOX 1 require specific maturation factors, DUOXA1 and DUOXA2, to be functionally expressed at the apical follicular membrane, the site for hormonogenesis. DUOX2/DUOX 1 are major components of H2O2-generating system and generate H2O2 which is required for activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. DUOX2 plays a role in thyroid hormones synthesis and LPO-mediated antimicrobial defense mucosal surface. It has its own peroxidase activity through its N-terminal peroxidase-like domain which is inhibited by aminobenzohydrazide and the NADPH oxidase activity is calcium-dependent. DUOX2 is expressed in thyrocytes, intestine, kidney, liver, lung, pancreas, prostate, salivary glands, testis etc. and is induced by forskolin, thyrotropin and the Th1-specific cytokine IFNG/IFN-gamma. Defects in DUOX2 have been linked to thyroid dyshormonogenesis 6 (TDH6) characterized by defective conversion of accumulated iodide to organically bound iodine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC-P

Publications for DUOX2 Antibody (NB110-61576)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Porcine
(1)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DUOX2 Antibody (NB110-61576). (Showing 1 - 2 of 2 FAQs).

  1. Hi, I am looking for an antibody to human DUOX2 that will work on human formalin fixed paraffin embedded sections. I notice from your website that you have several recommended for WB and wondered if you have any experience of their use in other areas?
    • At this time we do not have an antibody for DUOX2 that works in IHC paraffin embedded sections. If you choose to try one in this application we have our Innovator's Reward program where we reward our customers for trying antibodies in previously untested applications and species.
  2. Can you please let me know the homology of the immunogen peptide with mouse, for NB110-61576? Here is a link to the mouse DUOX2 protein information: ncbi
    • The homology is 84.6%.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-61576AF405
Alexa Fluor 488 Labeled NB110-61576AF488
Alexa Fluor 647 Labeled NB110-61576AF647
Alexa Fluor 700 Labeled NB110-61576AF700
Biotin Labeled NB110-61576B
DyLight 405 Labeled NB110-61576V
DyLight 488 Labeled NB110-61576G
DyLight 550 Labeled NB110-61576R
DyLight 650 Labeled NB110-61576C
FITC Labeled NB110-61576F
HRP Labeled NB110-61576H

Additional DUOX2 Products

DUOX2 NB110-61576

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Bioinformatics

Gene Symbol DUOX2