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DUOX1 Antibody

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Immunohistochemistry-Paraffin: DUOX1 Antibody [NB100-93521] - Staining of Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry-Paraffin: DUOX1 Antibody [NB100-93521] - Staining of Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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DUOX1 Antibody Summary

Immunogen
Peptide with sequence EKFQRSCLHQTVQ corresponding to internal region according to NP_059130.2, NP_787954.1.
Epitope
EKFQRSCLHQTVQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DUOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments in human placenta and thyroid gland lysates gave no specific signal but low background. IHC-P: Human thyroid shows staining of membranes in epithelial cells of the gland. WB reactivity reported in (PMID: 27526200).
Reviewed Applications
Read 1 Review rated 3
using
NB100-93521 in the following applications:
Publications
Read Publications using
NB100-93521 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for DUOX1 Antibody

  • dual oxidase 1
  • DUOX
  • EC 1.11.1.-
  • EC 1.6.3.1
  • Large NOX 1
  • LNOX1flavoprotein NADPH oxidase
  • Long NOX 1
  • MGC138840
  • NADPH thyroid oxidase 1
  • nicotinamide adenine dinucleotide phosphate oxidase
  • NOXEF1
  • THOX1MGC138841
  • Thyroid oxidase 1

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: DUOX2 Antibody [NB110-61576] - Detection of DUOX2 in A549 in cell lysate.
Immunocytochemistry/Immunofluorescence: DUOX2 Antibody [NB110-61576] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DUOX at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Western Blot: Thyroid Peroxidase Antibody [NBP1-97765] - (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-97765] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western Blot: NCF1 Antibody [NB100-790] - Staining of U937 (A), Daudi (B), U251 (C) and negative control A431 (D) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.2, 0.01, and 0.3 ug/mL respectively. Detected by chemiluminescence.
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Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. p<0.05. The identities of bands at intermediate molecular weight are unknown. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/omcl/2018/8238459/), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: NOX1 Antibody [NBP1-31546] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-NOX1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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(1 Review)
(22 Publications)
Species: Hu, Mu, Rt
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
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Western Blot: Pleckstrin Antibody [NB100-1343] - Staining of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Pleckstrin Antibody [NB100-1343] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ING1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ING1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line,PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ERK2 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu, Mu, Rt
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Angiotensin II/III
Antibody (Ang II E7 ( ...
NB100-62346
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. Western blot analysis for protein expressions of intrarenal RAS components in the two groups of mice. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Immunohistochemistry: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Protein expressions of intrarenal RAS components in two groups of mice. (A) Immunohistochemical analysis of intrarenal RAS expression position in the two groups of mice. AGT immunoreactivity in the proximal tubular cells, Ang II immunoreactivity in both glomerular and tubular cells, renin immunoreactivity in juxtaglomerular apparatus cells, ACE immunoreactivity in brush border membranes of proximal tubules as well as AT1 and AT2 immunoreactivity in the proximal tubules were increased in the HF group when compared to the control group. Image collected and cropped by CiteAb from the following publication (lipidworld.biomedcentral.com/articles/10.1186/1476-511X-12-49), licensed under a CC-BY licence.
Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).
(14 Publications)
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(7 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(23 Publications)
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow

Publications for DUOX1 Antibody (NB100-93521)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for DUOX1 Antibody (NB100-93521) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-93521:
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Images Ratings Applications Species Date Details
  3
reviewed by:
Melissa Kemp
 WB Human 04/19/2010
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Summary

ApplicationWestern Blot
Sample TestedJurkat whole cell lysate, Sample Amount: 40ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: Rockland near-infrared blocking buffer (Rockland Immunochemical), Blocking Time: 1 hr, Blocking Temp: RT

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: rabbit anti-goat, Secondary Ab Dilution Ratio: 1:15000
Secondary Manufacturer Cat#Rockland Immunochemicals #605-431-013

Details

Detection NotesDetection Method: Li-Cor Odyssey Near-infrared detection, Specific Bands: ~190 kDa, Non-Specific Bands: several

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DUOX1 Antibody (NB100-93521) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Array NB100-93521

Bioinformatics Tool for DUOX1 Antibody (NB100-93521)

Discover related pathways, diseases and genes to DUOX1 Antibody (NB100-93521). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for DUOX1 Antibody (NB100-93521)

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Blogs on DUOX1

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Recent Reviews

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 Melissa Kemp
04/19/2010
Application: WB
Species: Human
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Bioinformatics

Gene Symbol DUOX1
Entrez
Uniprot