DUOX1 Antibody

Images

 
Immunohistochemistry-Paraffin: DUOX1 Antibody [NB100-93521] - (4ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DUOX1 Antibody Summary

Immunogen
Peptide with sequence EKFQRSCLHQTVQ corresponding to internal region according to NP_059130.2, NP_787954.1.
Epitope
EKFQRSCLHQTVQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DUOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments in human placenta and thyroid gland lysates gave no specific signal but low background. IHC-P: Human thyroid shows staining of membranes in epithelial cells of the gland.
Reviewed Applications
Read 1 Review rated 3
using
NB100-93521 in the following applications:

Publications
Read Publications using
NB100-93521 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for DUOX1 Antibody

  • dual oxidase 1
  • DUOX
  • EC 1.11.1.-
  • EC 1.6.3.1
  • Large NOX 1
  • LNOX1flavoprotein NADPH oxidase
  • Long NOX 1
  • MGC138840
  • NADPH thyroid oxidase 1
  • nicotinamide adenine dinucleotide phosphate oxidase
  • NOXEF1
  • THOX1MGC138841
  • Thyroid oxidase 1

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DUOX1 Antibody (NB100-93521)(3)

Review for DUOX1 Antibody (NB100-93521) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-93521:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Melissa Kemp
WB Human 04/19/2010
View

Summary

ApplicationWestern Blot
Sample TestedJurkat whole cell lysate, Sample Amount: 40ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: Rockland near-infrared blocking buffer (Rockland Immunochemical), Blocking Time: 1 hr, Blocking Temp: RT

Primary Anitbody

Dilution RatioDilution Ratio: 1:1000, Incubation Dilution Buffer: blocking buffer, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: rabbit anti-goat, Secondary Ab Dilution Ratio: 1:15000
Secondary Manufacturer Cat#Rockland Immunochemicals #605-431-013

Details

Detection NotesDetection Method: Li-Cor Odyssey Near-infrared detection, Specific Bands: ~190 kDa, Non-Specific Bands: several

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DUOX1 Antibody (NB100-93521) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on DUOX1

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Melissa Kemp
04/19/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol DUOX1
Entrez
Uniprot