DUOX1 Antibody

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Immunohistochemistry-Paraffin: DUOX1 Antibody [NB100-93521] - Staining of Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DUOX1 Antibody Summary

Immunogen
Peptide with sequence EKFQRSCLHQTVQ corresponding to internal region according to NP_059130.2 , NP_787954.1.
Epitope
EKFQRSCLHQTVQ
Specificity
This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DUOX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot
Application Notes
WB: Preliminary experiments in human placenta and thyroid gland lysates gave no specific signal but low background. IHC-P: Human thyroid shows staining of membranes in epithelial cells of the gland. WB reactivity reported in (PMID: 27526200).
Reviewed Applications
Read 1 Review rated 3
using
NB100-93521 in the following applications:

Publications
Read Publications using
NB100-93521 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DUOX1 Antibody

  • dual oxidase 1
  • DUOX
  • EC 1.11.1.-
  • EC 1.6.3.1
  • Large NOX 1
  • LNOX1flavoprotein NADPH oxidase
  • Long NOX 1
  • MGC138840
  • NADPH thyroid oxidase 1
  • nicotinamide adenine dinucleotide phosphate oxidase
  • NOXEF1
  • THOX1MGC138841
  • Thyroid oxidase 1

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUOX1 Antibody (NB100-93521)(3)

Review for DUOX1 Antibody (NB100-93521) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-93521:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  3
reviewed by:
Melissa Kemp
WB Human 04/19/2010
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Summary

ApplicationWestern Blot
Sample TestedJurkat whole cell lysate, Sample Amount: 40ug
SpeciesHuman

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FAQs for DUOX1 Antibody (NB100-93521) (0)

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Melissa Kemp
04/19/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol DUOX1
Entrez
Uniprot