DUOX1 Antibody

Immunohistochemistry-Paraffin: DUOX1 Antibody [NB100-93521] - Staining of Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DUOX1 Antibody Summary

• Description: Novus Biologicals Goat DUOX1 Antibody (NB100-93521) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DUOX1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence EKFQRSCLHQTVQ corresponding to internal region according to NP_059130.2 , NP_787954.1.
• Epitope: EKFQRSCLHQTVQ
• Specificity: This antibody is expected to recognise both reported isoforms (NP_059130.2 ; NP_787954.1) which are identical;
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DUOX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot
• Application Notes: WB: Preliminary experiments in human placenta and thyroid gland lysates gave no specific signal but low background. IHC-P: Human thyroid shows staining of membranes in epithelial cells of the gland. WB reactivity reported in (PMID: 27526200).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DUOX1 Antibody

• dual oxidase 1
• DUOX
• EC 1.11.1.-
• EC 1.6.3.1
• Large NOX 1
• LNOX1flavoprotein NADPH oxidase
• Long NOX 1
• MGC138840
• NADPH thyroid oxidase 1
• nicotinamide adenine dinucleotide phosphate oxidase
• NOXEF1
• THOX1MGC138841
• Thyroid oxidase 1

Background

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DUOX1 Antibody (NB100-93521)(3)

Publications using NB100-93521

• Rivet CA, Kniss-James AS, Gran MA et al. Calcium Dynamics of Ex Vivo Long-Term Cultured CD8+ T Cells Are Regulated by Changes in Redox Metabolism. PLoS ONE. 2016-08-16 [PMID: 27526200] (WB, Human)
• Kippner LE, Finn NA, Shukla S, Kemp ML. Systemic remodeling of the redox regulatory network due to RNAi perturbations of glutaredoxin 1, thioredoxin 1, and glucose-6-phosphate dehydrogenase. BMC Systems Biology;5(1):164. 2011-10-13 [PMID: 21995976]
• Harper RW, Xu C, Eiserich JP et al. Differential regulation of dual NADPH oxidases/peroxidases, Duox1 and Duox2, by Th1 and Th2 cytokines in respiratory tract epithelium. FEBS Lett 2005-08-29 [PMID: 16111680]

Review for DUOX1 Antibody (NB100-93521) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Melissa Kemp WB Human 04/19/2010

Summary

• Application: Western Blot
• Sample Tested: Jurkat whole cell lysate, Sample Amount: 40ug
• Species: Human

Bioinformatics

• Gene Symbol: DUOX1
• Entrez:
  • 53905(Human)
• Uniprot:
  • NP_059130.2(Human)