OPA1 Antibody - BSA Free

Images

 
Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: OPA1 Antibody [NB110-55290] - Depletion of Oma1 in fish affects development. Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a-f) ...read more
Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunohistochemistry: OPA1 Antibody [NB110-55290] - Staining in prostatic smooth muscle and glandular epithelium. Human Prostate 40X magnification.
Western Blot: OPA1 Antibody [NB110-55290] - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.
Western Blot: OPA1 Antibody [NB110-55290] - Analysis of OPA1 in porcine retinal pigment epithelium lysate using anti-OPA1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Ze, PmSpecies Glossary
Applications WB, Simple Western, IB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

OPA1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region within residues 500-600 of human OPA1. [Swiss-Prot: O60313]
Localization
Mitochondrion; mitochondrial inner membrane; single-pass membrane protein. Mitochondrion; mitochondrial intermembrane space.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Simple Western 20 ug/ml
  • Western Blot 2 ug/ml
Application Notes
Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:34923139).In Western blot, a band is seen at approx. 111 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by size. Use in immunoblotting reported in scientific literature (PMID: 25224038)..
Theoretical MW
111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
OPA1 Peptide (NB110-55290PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-55290 in the following applications:

Publications
Read Publications using
NB110-55290 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • 1 publication
  • SW
    1 publication
  • WB
    25 publications

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 23516612, 26365306).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OPA1 Antibody - BSA Free

  • BERHS
  • EC 3.6.5.5
  • FLJ12460
  • KIAA0567dynamin-like 120 kDa protein, mitochondrial
  • LargeG
  • lilr3
  • MGM1
  • mitochondrial dynamin-like GTPase
  • MTDPS14
  • NPG
  • NPGlargeG
  • NTG
  • NTGmitochondrial dynamin-like 120 kDa protein
  • OPA1
  • optic atrophy 1 (autosomal dominant)
  • Optic atrophy protein 1

Background

OPA1 (optic atrophy-1) is a member of dynamin family of large GTPases and is a major player in fusion of the inner mitochondrial membrane and maintenance of cristae architecture, events which are orchestrated through oligomerization of differentially processed forms of OPA1. It exists as a single-pass membrane protein in the mitochondrion inner membrane as well as in soluble forms in mitochondrion intermembrane space, and is expressed in retina, brain, testis, heart, skeletal muscles etc. It binds with PARL and interacts with CHCHD3 as well as IMMT (preferentially with soluble OPA1 forms). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into mitochondrial intermembrane space. OPA1 form S1: inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Defects in OPA1 have been linked to optic atrophy type 1 (OPA1) and dominant optic atrophy plus syndrome (DOA+).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-55290
Species: Hu, Mu, Rt, Po, Ch, Ze, Pm
Applications: WB, Simple Western, IB, ICC/IF, IHC, IHC-P

Publications for OPA1 Antibody (NB110-55290)(36)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Zebrafish.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, SW, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
SW
(1)
WB
(25)
All Applications
Filter By Species
Human
(9)
Mouse
(10)
Rat
(4)
Porcine
(3)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using NB110-55290 Applications Species
Bus C, Geisler S, Feldkaemper M, Flores-Romero H PINK1 Regulates Dopamine and Lipids at Mitochondria to Maintain Synapses and Neuronal Function bioRxiv 2019 Oct 22 (WB, Mouse) WB Mouse
Chidipi B, Angulo MB, Shah SI et al. The dynamin-related protein 1 is decreased and the mitochondrial network is altered in Friedreich's ataxia cardiomyopathy The international journal of biochemistry & cell biology Dec 16 2021 [PMID: 34923139] (WB, ICC/IF, Human) WB, ICC/IF Human
Gomez-Valades Ag, Pozo M, Varela L Et Al. Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca2+ homeostasis with adipose tissue lipolysis Cell metabolism Jul 27 2021 [PMID: 34343501]
Senthil K, Morgan R, Hefti M et al. Haemodynamic-directed cardiopulmonary resuscitation promotes mitochondrial fusion and preservation of mitochondrial mass after successful resuscitation in a pediatric porcine model Resuscitation Plus Jun 1 2021
Chidipi B, Shah SI, Reiser M et al. All-Trans Retinoic Acid Increases DRP1 Levels and Promotes Mitochondrial Fission Cells May 14 2021 [PMID: 34068960] (WB, Human, Mouse) WB Human, Mouse
Sakiyama H, Li L, Kuwahara-Otani S et al. A lack of ChREBP inhibits mitochondrial cristae formation in brown adipose tissue Molecular and cellular biochemistry May 21 2021 [PMID: 34021470]
e Silva AC Neuro-Resilient Function of PRMT8 Against Cellular Stress Thesis Jan 1 2021
Harper S. A, Bassler J. R, et al. Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial. Exp Gerontol 2021-01-01 [PMID: 33068691] (SW, Human) SW Human
Li G, Chan Y. L, et al. E-cigarettes damage the liver and alter nutrient metabolism in pregnant mice and their offspring. Ann N Y Acad Sci 2020-09-01 [PMID: 32557680] (WB, Mouse) WB Mouse
Show All 36 Publications.

Reviews for OPA1 Antibody (NB110-55290) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using NB110-55290:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Other
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot OPA1 NB110-55290
Enlarge
5
reviewed by:
Verified Customer
WB Other 06/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedRetinal pigment epithelium lysate
SpeciesOther
LotD

Blocking

Blocking Details5% milk in TBST, 1hr, room temperature

Primary Anitbody

Dilution Ratio1:500 in TBST, 1hr, room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit, HRP
Secondary Manufacturer Cat#4050-08
Secondary Concentration1:10,000

Details

Detection NotesECL/film, 2min exposure
  5
reviewed by:
Verified Customer
WB Other 01/19/2010
View

Summary

ApplicationWestern Blot
Sample TestedCOS-1 cell, Sample Amount: 30 ug
SpeciesOther
LotB1

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat dry milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio:1/1000,Incubation Dilution Buffer:5% non-fat dry milk in TBST,Incubation Time:overnight,Incubation Temp:4 deg Cel

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat-anti-Rabbit HRP pAb, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: ECL, Exposure Time: 10min, Specific Bands: 110 kDa

Product General Protocols

View specific protocols for OPA1 Antibody (NB110-55290): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OPA1 Antibody (NB110-55290) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for OPA1 Antibody (NB110-55290)

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Research Areas for OPA1 Antibody (NB110-55290)

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Blogs on OPA1.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

Understanding OPA1 and Mitochondrial Function
OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Verified Customer
06/04/2015
Application: WB
Species: Other

 
Verified Customer
01/19/2010
Application: WB
Species: Other

Bioinformatics

Gene Symbol OPA1