OPA1 Antibody - BSA Free

Simple Western: OPA1 Antibody [NB110-55290] - Lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: OPA1 Antibody [NB110-55290] - Depletion of Oma1 in fish affects development. Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a-f) and Cox4 (mitochondrial abundance marker) was analyzed with respective antibodies. Actin and Ponceau S staining were loading controls. Source data (full-length blots) are available online in Supplementary information. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep13989), licensed under a CC-BY license.

Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-OPA1 in 1% blocking buffer. Precision Plus Protein All Blue molecular weight markers (BioRad) were detected with 1 ug/ml Anti-Blue Marker antibody (NBP2-33376). The protein ladder was detected with an anti-mouse HRP secondary antibody and OPA1 with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry: OPA1 Antibody [NB110-55290] - Staining in prostatic smooth muscle and glandular epithelium. Human Prostate 40X magnification.

Western Blot: OPA1 Antibody [NB110-55290] - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.

Western Blot: OPA1 Antibody [NB110-55290] - Analysis of OPA1 in porcine retinal pigment epithelium lysate using anti-OPA1 antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Ch, Ze, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

OPA1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal region within residues 500-600 of human OPA1. [Swiss-Prot: O60313]
• Localization: Mitochondrion; mitochondrial inner membrane; single-pass membrane protein. Mitochondrion; mitochondrial intermembrane space.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting reported in scientific literature (PMID 25224038)
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 34923139)
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Simple Western 20 ug/ml
  • Western Blot 2 ug/ml
• Application Notes: In Western blot, a band is seen at approx. 111 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by size.
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  OPA1 Peptide (NB110-55290PEP)

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 23516612, 26365306).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OPA1 Antibody - BSA Free

• BERHS
• EC 3.6.5.5
• FLJ12460
• KIAA0567dynamin-like 120 kDa protein, mitochondrial
• LargeG
• lilr3
• MGM1
• mitochondrial dynamin-like GTPase
• MTDPS14
• NPG
• NPGlargeG
• NTG
• NTGmitochondrial dynamin-like 120 kDa protein
• OPA1
• optic atrophy 1 (autosomal dominant)
• Optic atrophy protein 1

Background

OPA1 (optic atrophy-1) is a member of dynamin family of large GTPases and is a major player in fusion of the inner mitochondrial membrane and maintenance of cristae architecture, events which are orchestrated through oligomerization of differentially processed forms of OPA1. It exists as a single-pass membrane protein in the mitochondrion inner membrane as well as in soluble forms in mitochondrion intermembrane space, and is expressed in retina, brain, testis, heart, skeletal muscles etc. It binds with PARL and interacts with CHCHD3 as well as IMMT (preferentially with soluble OPA1 forms). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into mitochondrial intermembrane space. OPA1 form S1: inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Defects in OPA1 have been linked to optic atrophy type 1 (OPA1) and dominant optic atrophy plus syndrome (DOA+).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OPA1 Antibody (NB110-55290)(41)

Publications using NB110-55290

• Saito ER, Warren CE, Hanegan CM et al. A Novel Ketone-Supplemented Diet Improves Recognition Memory and Hippocampal Mitochondrial Efficiency in Healthy Adult Mice Metabolites 2022-10-25 [PMID: 36355101] (WB, Mouse)
• Wang D, Kuang Y, Wan Z et al. Aspartate Alleviates Colonic Epithelial Damage by Regulating Intestinal Stem Cell Proliferation and Differentiation via Mitochondrial Dynamics Molecular nutrition & food research 2022-10-30 [PMID: 36310136] (WB)
• Yao J, Takenaga K, Koshikawa N et al. Anticancer effect of a pyrrole-imidazole polyamide-triphenylphosphonium conjugate selectively targeting a common mitochondrial DNA cancer risk variant in cervical cancer cells International journal of cancer 2022-10-10 [PMID: 36214789]
• Trewin AJ, Silver J, Dillon HT et al. Long non-coding RNA Tug1 modulates mitochondrial and myogenic responses to exercise in skeletal muscle BMC biology 2022-07-18 [PMID: 35850762] (WB, Mouse)
• Ramasubramanian B, Griffith C, Hanson M et al. Protective Effects of Chaya against Mitochondrial and Synaptic Toxicities in the Type 2 Diabetes Mouse Model TallyHO Cells 2022-02-21 [PMID: 35203393] (IF/IHC, WB, Mouse)
• Li J, Zhang Z, Wang L et al. Maresin 1 Attenuates Lipopolysaccharide-Induced Acute Kidney Injury via Inhibiting NOX4/ROS/NF-kappaB Pathway Frontiers in pharmacology 2021-12-10 [PMID: 34955852]
• Chidipi B, Angulo MB, Shah SI et al. The dynamin-related protein 1 is decreased and the mitochondrial network is altered in Friedreich's ataxia cardiomyopathy The international journal of biochemistry & cell biology 2021-12-16 [PMID: 34923139] (ICC/IF, WB, Human)
• Senthil K, Morgan R, Hefti M et al. Haemodynamic-directed cardiopulmonary resuscitation promotes mitochondrial fusion and preservation of mitochondrial mass after successful resuscitation in a pediatric porcine model Resusc Plus 2021-07-05 [PMID: 34223382]
• Chidipi B, Shah SI, Reiser M et al. All-Trans Retinoic Acid Increases DRP1 Levels and Promotes Mitochondrial Fission Cells 2021-05-14 [PMID: 34068960] (WB, Human, Mouse)
• Gomez-Valades Ag, Pozo M, Varela L Et Al. Mitochondrial cristae-remodeling protein OPA1 in POMC neurons couples Ca2+ homeostasis with adipose tissue lipolysis Cell metabolism 2021-07-27 [PMID: 34343501]
• Sakiyama H, Li L, Kuwahara-Otani S et al. A lack of ChREBP inhibits mitochondrial cristae formation in brown adipose tissue Molecular and cellular biochemistry 2021-05-21 [PMID: 34021470]
• Harper S. A, Bassler J. R, et al. Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial. Exp Gerontol 2021-01-01 [PMID: 33068691] (SW, Human)
• Li G, Chan Y. L, et al. E-cigarettes damage the liver and alter nutrient metabolism in pregnant mice and their offspring. Ann N Y Acad Sci 2020-09-01 [PMID: 32557680] (WB, Mouse)
• e Silva AC Neuro-Resilient Function of PRMT8 Against Cellular Stress Thesis 2021-01-01
• Bus C, Geisler S, Feldkaemper M, Flores-Romero H PINK1 Regulates Dopamine and Lipids at Mitochondria to Maintain Synapses and Neuronal Function bioRxiv 2019-10-22 (WB, Mouse)
• Ren Z, Guo C, He H et al. Effects of deoxynivalenol on mitochondrial dynamics and autophagy in pig spleen lymphocytes Food Chem. Toxicol. 2020-04-14 [PMID: 32302720] (WB, Porcine)
• Raynor JL, Liu C, Dhungana Y et al. Hippo/Mst signaling coordinates cellular quiescence with terminal maturation in iNKT cell development and fate decisions J. Exp. Med. 2020-06-01 [PMID: 32289155] (WB, Mouse)
• Zakarya R, Sapkota A, Chan YL et al. Nitroxides affect neurological deficits and lesion size induced by a rat model of traumatic brain injury Nitric Oxide 2020-04-01 [PMID: 32061903] (WB, Rat)
• Ge Y, Shi X, Boopathy S et al. Two forms of Opa1 cooperate to complete fusion of the mitochondrial inner-membrane Elife 2020-01-11 [PMID: 31922487]
• Chao T, Gomez BI, Heard TC et al. Burn-Induced Reductions in Mitochondrial Abundance and Efficiency are More Pronounced with Small Volumes of Colloids in Swine Am. J. Physiol., Cell Physiol. 2019-09-18 [PMID: 31532719] (WB, Porcine)
• Chao T, Burmeister D M et al. Oxidative pathophysiology following volumetric muscle loss injury in a porcine model. J Appl Physiol (1985) 2019-01-06 [PMID: 30920884] (WB, Porcine)
• Chang JC, Chang HS, Wu YC et al. Mitochondrial transplantation regulates antitumour activity, chemoresistance and mitochondrial dynamics in breast cancer J. Exp. Clin. Cancer Res. 2019-01-23 [PMID: 30674338] (WB, Human)
• Sikorski K, Mehta A et al. A high-throughput pipeline for validation of antibodies. Nat Methods 2018-01-11 [PMID: 30377371] (Human)
  
  Details:
  Antibody validation based on denaturing gel electrophoresis of biotinylated cell lysates (PAGE) followed by mass spectrometry (MS) and antibody array analysis (MAP).
• Chen H, Chan YL, Linnane C et al. L-Carnitine and extendin-4 improve outcomes following moderate brain contusion injury. Sci Rep 2018-07-25 [PMID: 30046063] (WB, Rat)
• Du X, Wen J, Wang Y et al. Hippo/Mst signalling couples metabolic state and immune function of CD8alpha+ dendritic cells Nature 2018-06-01 [PMID: 29849151] (WB, Mouse)
• Chan YL, Saad S, Al-Odat I et al. Maternal L-Carnitine Supplementation Improves Brain Health in Offspring from Cigarette Smoke Exposed Mothers. Front Mol Neurosci. 2017-02-28 [PMID: 28243190] (WB, Mouse)
• Tan LX, Toops KA, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium Proc. Natl. Acad. Sci. U.S.A. 2016-08-02 [PMID: 27432952] (WB)
• Bohovych I, Fernandez MR, Rahn JJ et al. Metalloprotease OMA1 Fine-tunes Mitochondrial Bioenergetic Function and Respiratory Supercomplex Stability. Sci Rep 2015-09-14 [PMID: 26365306] (WB, Zebrafish)
• Yue M, Hinkle KM, Davies P et al. Progressive dopaminergic alterations and mitochondrial abnormalities in LRRK2 G2019S knock-in mice Neurobiol. Dis. 2015-03-31 [PMID: 25836420] (WB, Mouse)
• Lee KP, Jin Shin Y, Chun Cho S et al. Peroxiredoxin 3 has a Crucial Role in the Contractile Function of Skeletal Muscle via Regulating Mitochondrial Homeostasis. Free Radic. Biol. Med. 2014-09-12 [PMID: 25224038]
• Lionetti L, Mollica MP, Donizzetti I et al. High-lard and high-fish-oil diets differ in their effects on function and dynamic behaviour of rat hepatic mitochondria. PLoS ONE 2014-03-25 [PMID: 24663492] (WB, IHC-P, Rat)
• Hara H, Araya J, Ito S et al. Mitochondrial fragmentation in cigarette smoke-induced bronchial epithelial cell senescence. Am J Physiol Lung Cell Mol Physiol. 2013-11-01 [PMID: 24056969] (WB, Human)
• Ussakli CH, Ebaee A, Binkley J et al. Mitochondria and Tumor Progression in Ulcerative Colitis. J Natl Cancer Inst 2013-07-12 [PMID: 23852949] (IF/IHC, Human)
• Rahn JJ, Stackley KD, Chan SS. Opa1 is required for proper mitochondrial metabolism in early development PLoS One 2013-01-01 [PMID: 23516612] (WB, Zebrafish)
• Johnson D, Allman E, Nehrke K. Regulation of acid-base transporters by reactive oxygen species following mitochondrial fragmentation. Am J Physiol Cell Physiol;302(7):C1045-54. 2012-04-01 [PMID: 22237403] (WB, Rat)
• Vallon M, Aubele P, Janssen K-P, Essler M. Thrombin-induced shedding of tumor endothelial marker 5 and exposure of its RGD motif are regulated by cell surface protein disulfide isomerase. The Biochemical Journal. 2011-10-20 [PMID: 22013897] (WB, Human)
• Dai DF, Hsieh EJ, Liu Y, Chen T, Beyer RP, Chin MT, MacCoss MJ, Rabinovitch PS. Mitochondrial proteome remodelling in pressure overload-induced heart failure: the role of mitochondrial oxidative stress. Cardiovasc Res;93(1):79-88. 2012-01-01 [PMID: 22012956] (WB, Mouse)
• Tang D, Kang R, Livesey KM et al. High-mobility group box 1 is essential for mitochondrial quality control Cell Metab 2011-06-01 [PMID: 21641551] (WB, Mouse)
• Yoon Y, Kim S, Han Y et al. Protect TUDCA stimulated CKD-derived hMSCs against the CKD-Ischemic disease via upregulation of PrPC. bioRxiv 2018-08-28 (WB, Human)
• Trushina E, Nemutlu E, Zhang S et al. Defects in mitochondrial dynamics and metabolomic signatures of evolving energetic stress in mouse models of familial Alzheimer's disease. PLoS One. 2012-01-01 [PMID: 22393443] (WB, Mouse)

Reviews for OPA1 Antibody (NB110-55290) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Li Xuan Tan WB Other 06/04/2015

Summary

• Application: Western Blot
• Sample Tested: Retinal pigment epithelium lysate
• Species: Other
• Lot: D

reviewed by: System Admin WB Other 01/19/2010

Summary

• Application: Western Blot
• Sample Tested: COS-1 cell, Sample Amount: 30 ug
• Species: Other
• Lot: B1

Bioinformatics

• Gene Symbol: OPA1