OPA1 Antibody

Images

 
Western Blot: OPA1 Antibody [NB110-55290] - Total protein from Human HeLa cells, Mouse MEF cells and Rat Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Immunohistochemistry: OPA1 Antibody [NB110-55290] - Staining in prostatic smooth muscle and glandular epithelium. Human Prostate 40X magnification.
Western Blot: OPA1 Antibody [NB110-55290] - Detection of OPA1 protein in post-nuclear extracts of mouse embryonic fibroblasts.
Western Blot: OPA1 Antibody [NB110-55290] - analysis of OPA1 in porcine retinal pigment epithelium lysate using anti-OPA1 antibody. Image from verified customer review.
Simple Western: OPA1 Antibody [NB110-55290] - Simple Western lane view shows a specific band for OPA1 in 0.5 mg/ml of MEF lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Pm, ZeSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

OPA1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region within residues 500-600 of human OPA1. [Swiss-Prot# O60313]
Localization
Mitochondrion; mitochondrial inner membrane; single-pass membrane protien. Mitochondrion; mitochondrial intermembrane space.
Predicted Species
Zebrafish (94%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Simple Western 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Application Notes
This OPA1 antibody is useful for Immunohistochemistry on paraffin-embedded sections and Western blot analysis, where a band is seen at approx. 111 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Lysate (NB820-59177)
Control Peptide
OPA1 Peptide (NB110-55290PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-55290 in the following applications:

Publications
Read Publications using
NB110-55290 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    12 publications

Reactivity Notes

Human, mouse, rat and chicken. Predicted to react with primate based on 100% sequence homology. Immunogen sequence has 94% homology to Zebrafish.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OPA1 Antibody

  • BERHS
  • EC 3.6.5.5
  • FLJ12460
  • KIAA0567dynamin-like 120 kDa protein, mitochondrial
  • LargeG
  • lilr3
  • MGM1
  • mitochondrial dynamin-like GTPase
  • MTDPS14
  • NPG
  • NPGlargeG
  • NTG
  • NTGmitochondrial dynamin-like 120 kDa protein
  • OPA1
  • optic atrophy 1 (autosomal dominant)
  • Optic atrophy protein 1

Background

OPA1 (optic atrophy-1) is a member of dynamin family of large GTPases and is a major player in fusion of the inner mitochondrial membrane and maintenance of cristae architecture, events which are orchestrated through oligomerization of differentially processed forms of OPA1. It exists as a single-pass membrane protein in the mitochondrion inner membrane as well as in soluble forms in mitochondrion intermembrane space, and is expressed in retina, brain, testis, heart, skeletal muscles etc. It binds with PARL and interacts with CHCHD3 as well as IMMT (preferentially with soluble OPA1 forms). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into mitochondrial intermembrane space. OPA1 form S1: inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion. Defects in OPA1 have been linked to optic atrophy type 1 (OPA1) and dominant optic atrophy plus syndrome (DOA+).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OPA1 Antibody (NB110-55290)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(12)
All Applications
Filter By Species
Human
(3)
Mouse
(5)
Rat
(2)
Zebrafish
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NB110-55290 Applications Species
Chan YL, Saad S, Al-Odat I et al. Maternal L-Carnitine Supplementation Improves Brain Health in Offspring from Cigarette Smoke Exposed Mothers. Front Mol Neurosci. 2017 Feb 28 [PMID: 28243190] (WB, Mouse) WB Mouse
Tan LX, Toops KA, Lakkaraju A. Protective responses to sublytic complement in the retinal pigment epithelium Proc. Natl. Acad. Sci. U.S.A. 2016 Aug 02 [PMID: 27432952] (WB) WB
Bohovych I, Fernandez MR, Rahn JJ et al. Metalloprotease OMA1 Fine-tunes Mitochondrial Bioenergetic Function and Respiratory Supercomplex Stability. Sci Rep 2015 Sep 14 [PMID: 26365306] (WB, Zebrafish) WB Zebrafish
Yue M, Hinkle KM, Davies P et al. Progressive dopaminergic alterations and mitochondrial abnormalities in LRRK2 G2019S knock-in mice Neurobiol. Dis. 2015 Mar 31 [PMID: 25836420] (WB, Mouse) WB Mouse
Lee KP, Jin Shin Y, Chun Cho S et al. Peroxiredoxin 3 has a Crucial Role in the Contractile Function of Skeletal Muscle via Regulating Mitochondrial Homeostasis. Free Radic. Biol. Med. 2014 Sep 12 [PMID: 25224038]
Lionetti L, Mollica MP, Donizzetti I et al. High-lard and high-fish-oil diets differ in their effects on function and dynamic behaviour of rat hepatic mitochondria. PLoS ONE 3/25/2014 [PMID: 24663492] (WB, IHC-P, Rat) WB, IHC-P Rat
Hara H, Araya J, Ito S et al. Mitochondrial fragmentation in cigarette smoke-induced bronchial epithelial cell senescence. Am J Physiol Lung Cell Mol Physiol. 2013 Nov [PMID: 24056969] (WB, Human) WB Human
Ussakli CH, Ebaee A, Binkley J et al. Mitochondria and Tumor Progression in Ulcerative Colitis. J Natl Cancer Inst 2013 Jul 12 [PMID: 23852949] (IHC, Human) IHC Human
Rahn JJ, Stackley KD, Chan SS. Opa1 is required for proper mitochondrial metabolism in early development PLoS One 2013 [PMID: 23516612] (WB, Zebrafish) WB Zebrafish
Trushina E, Nemutlu E, Zhang S et al. Defects in Mitochondrial Dynamics and Metabolomic Signatures of Evolving Energetic Stress in Mouse Models of Familial Alzheimer's Disease PLoS ONE 2012 [PMID: 22393443] (WB, Mouse) WB Mouse
Show All 14 Publications.

Reviews for OPA1 Antibody (NB110-55290) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Other.

Reviews using NB110-55290:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Other
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot OPA1 NB110-55290
Enlarge
reviewed by:
Anonymous
WB Other 06/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedRetinal pigment epithelium lysate
SpeciesOther
LotD

Blocking

Blocking Details5% milk in TBST, 1hr, room temperature

Primary Anitbody

Dilution Ratio1:500 in TBST, 1hr, room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit, HRP
Secondary Manufacturer Cat#4050-08
Secondary Concentration1:10,000

Details

Detection NotesECL/film, 2min exposure
Western Blot OPA1 NB110-55290
Enlarge
reviewed by:
Anonymous
WB Other 01/19/2010
View

Summary

ApplicationWestern Blot
Sample TestedCOS-1 cell, Sample Amount: 30 ug
SpeciesOther
LotB1

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat dry milk in TBST, Blocking Time: 1 hour, Blocking Temp: 25 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio:1/1000,Incubation Dilution Buffer:5% non-fat dry milk in TBST,Incubation Time:overnight,Incubation Temp:4 deg Cel

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat-anti-Rabbit HRP pAb, Secondary Ab Dilution Ratio: 1/10000

Details

Detection NotesDetection Method: ECL, Exposure Time: 10min, Specific Bands: 110 kDa

Product General Protocols

View specific protocols for OPA1 Antibody (NB110-55290): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for OPA1 Antibody (NB110-55290) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-55290AF405
Alexa Fluor 488 Labeled NB110-55290AF488
Alexa Fluor 647 Labeled NB110-55290AF647
Alexa Fluor 700 Labeled NB110-55290AF700
Biotin Labeled NB110-55290B
DyLight 405 Labeled NB110-55290V
DyLight 488 Labeled NB110-55290G
DyLight 550 Labeled NB110-55290R
DyLight 650 Labeled NB110-55290C
FITC Labeled NB110-55290F
HRP Labeled NB110-55290H

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Bioinformatics Tool for OPA1 Antibody (NB110-55290)

Discover related pathways, diseases and genes to OPA1 Antibody (NB110-55290). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for OPA1 Antibody (NB110-55290)

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Pathways for OPA1 Antibody (NB110-55290)

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Research Areas for OPA1 Antibody (NB110-55290)

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Blogs on OPA1.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

Understanding OPA1 and Mitochondrial Function
OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
06/04/2015
Application: WB
Species: Other

Anonymous
01/19/2010
Application: WB
Species: Other

Bioinformatics

Gene Symbol OPA1
Entrez