DISC1 Antibody [DyLight 488]

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 488

DISC1 Antibody [DyLight 488] Summary

• Immunogen: A synthetic peptide made to an internal region (within residues 400-500) of the rat DISC1 protein. [Swiss-Prot# Q810H6]
• Localization: DISC-1 localizes to the nucleus, whereas mutant DISC-1 localization occurs mainly in the cytoplasm. It co-localizes with the following organelle structure antibodies: PD1, calnexin, GIANTIN, 58K, Rab4, LAMP-1, RAP1, KDEL, and catalase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DISC1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: This DISC1 antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry paraffin embedded sections.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24560582) The theoretical molecular weight of DISC1 is ~90 kDa. Preliminary Western Blot studies have been performed with this antibody, where several non-specific bands are seen. Due to alternative splicing, DISC1 may also run at ~75 and 100 kDa. In ICC/IF, cytoplasmic staining was observed in PC12 cells.

Reactivity Notes

Human, rat and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DISC1 Antibody [DyLight 488]

• C1orf136
• DISC1
• disrupted in schizophrenia 1 protein
• disrupted in schizophrenia 1
• FLJ13381
• FLJ21640
• FLJ25311
• FLJ41105
• KIAA0457
• SCZD9

Background

The 'Disrupted In Schizophrenia' gene locus DISC is associated with patients afflicted with schizophrenia as a result of chromosomal translocations. DISC-1 encodes a large protein predicted to contain a globular N-terminal domain and a helical C-terminal domain, both of which have the potential to form interactions with other proteins. DISC-1 interacts with proteins involved in the centrosome and cytoskeletal system, including MIPT3, MAP1A and NUDEL; proteins which localize receptors to membranes, including alpha-actinin 2 an beta 4-spectrin; and proteins which transduce signals from membrane receptors, including ATF-4 and ATF-5. Therefore, DISC-1 is thought to be involved in intracellular transport, neurite architecture and/or neuronal migration, all of which are thought to be pathogenic in the schizophrenic brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DISC1