PDE4B Antibody (OTI1D12)

Western Blot: PDE4B Antibody (1D12) [NBP2-01171] Analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: PDE4B Antibody (1D12) [NBP2-01171] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4B.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4B mouse monoclonal antibody.

Flow Cytometry: PDE4B Antibody (1D12) [NBP2-01171] - Analysis of Jurkat cells, using anti-PDE4B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: PDE4B Antibody (1D12) [NBP2-01171] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDE4B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDE4B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PDE4B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDE4B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDE4B mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PDE4B Antibody (1D12) [NBP2-01171] - Staining of paraffin-embedded Human pancreas tissue using anti-PDE4B mouse monoclonal antibody.

Flow Cytometry: PDE4B Antibody (1D12) [NBP2-01171] - Analysis of Hela cells, using anti-PDE4B antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: OTI1D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.62 mg/ml

PDE4B Antibody (OTI1D12) Summary

• Immunogen: Full length human recombinant protein of human PDE4B(NP_002591) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDE4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 83.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.62 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE4B Antibody (OTI1D12)

• cAMP-specific (phosphodiesterase E4 dunce homolog
• cAMP-specific 3'-5'-cyclic phosphodiesterase 4B
• cAMP-specific phosphodiesterase-4 B isoform
• DKFZp686F2182
• DPDE4
• Drosophila)
• dunce-like phosphodiesterase E4
• EC 3.1.4
• EC 3.1.4.17
• MGC126529
• PDE32
• PDE4B
• PDEIVB
• phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E4)
• phosphodiesterase 4B, cAMP-specific (dunce
• phosphodiesterase 4B, cAMP-specific
• phosphodiesterase E4 dunce homolog

Background

This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDE4B Antibody (NBP2-01171)(1)

Publication using NBP2-01171

• Varona S, Puertas L, GalAn M et al. Rolipram Prevents the Formation of Abdominal Aortic Aneurysm (AAA) in Mice: PDE4B as a Target in AAA Antioxidants 2021-03-16 [PMID: 33809405] (IHC-P, WB, Human)

Bioinformatics

• Gene Symbol: PDE4B
• Entrez:
  • 5142(Human)