NDE1 Antibody (2G11-1C11) - Azide and BSA Free

Western Blot: NDE1 Antibody (2G11-1C11) [H00054820-M01] - NDE1 monoclonal antibody (M01), clone 2G11-1C11 Analysis of NDE1 expression in Hela.

Immunocytochemistry/Immunofluorescence: NDE1 Antibody (2G11-1C11) [H00054820-M01] - Analysis of monoclonal antibody to NDE1 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: NDE1 Antibody (2G11-1C11) [H00054820-M01] - Detection limit for recombinant GST tagged NDE1 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2G11-1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NDE1 Antibody (2G11-1C11) - Azide and BSA Free Summary

• Immunogen: NDE1 (AAH01421, 1 a.a. ~ 335 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
• Specificity: NDE1 - nudE nuclear distribution gene E homolog 1 (A. nidulans)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NDE1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NDE1 Antibody (2G11-1C11) - Azide and BSA Free

• FLJ20101
• HOM-TES-87
• nuclear distribution protein nudE homolog 1
• nudE nuclear distribution gene E homolog 1 (A. nidulans)
• NudE
• NUDE1
• rat homolog

Background

The NDE1 gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This pr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDE1 Antibody (H00054820-M01)(5)

Publications using H00054820-M01

• Ana G, Bernardo O, Marco N et al. Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells. Curr Biol. 2022-08-27 [PMID: 36057259]
• Almeida AC, Soares-de-Oliveira J, Drpic D et al. Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals Cell reports 2022-04-05 [PMID: 35385739]
• Vanessa N, Margarida D, Domingos C et al. Centrosome-nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity. Mol Biol Cell. 2020-04-29 [PMID: 32348198]
• Doobin DJ, Kemal S, Dantas TJ, Vallee RB Severe NDE1-mediated microcephaly results from neural progenitor cell cycle arrests at multiple specific stages. Nat Commun 2016-08-24 [PMID: 27553190] (WB)
• Bradshaw NJ, Christie S, Soares DC et al. NDE1, NDEL1: Multimerisation, alternate splicing and DISC1 interaction. Neurosci Lett. 2008-11-07 [PMID: 19000741]

Bioinformatics

• Gene Symbol: NDE1
• Entrez:
  • 54820(Human)
• OMIM:
  • 609449(Human)
• Uniprot:
  • AAH01421(Human)