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Nudel Antibody (OTI5E11)

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Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.
Flow Cytometry: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nudel antibody, and then analyzed ...read more
Western Blot: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nudel (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Nudel mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Nudel mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Kidney tissue using anti-Nudel mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human liver tissue using anti-Nudel mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI5E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Order Details

Nudel Antibody (OTI5E11) Summary

Immunogen
Full length human recombinant protein of human NDEL1(NP_001020750) produced in HEk293 cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NDEL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Nudel Antibody (OTI5E11)

  • DKFZp451M0318
  • EOPA
  • MITAP1mitosin-associated protein MITAP1
  • Mitosin-associated protein 1
  • nuclear distribution protein nudE-like 1
  • nudE nuclear distribution gene E homolog (A. nidulans)-like 1
  • nudE nuclear distribution gene E homolog like 1 (A. nidulans)
  • nudE nuclear distribution gene E homolog like 1
  • NUDELendooligopeptidase A
  • NUDE-like protein
  • Protein Nudel

Background

This gene encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Nudel Antibody (NBP2-01086). (Showing 1 - 1 of 1 FAQ).

  1. I want to buy NBP2-01086, but I am confused by the antibody application. Does IHC means IHC-Frozen? I need this antibody who can be applied to IHC-Fr.
    • No, this antibody has not been tested for IHC-Fr application in our lab, however, as it worked well in IHC-P (which has more tough conditions for antigens-antibody reactivity compared to IHC-Fr) as well as ICC-IF, we strongly believe that this antibody should work in IHC-Fr application also. Moreover, if you chose to test this antibody in IHC-Fr, you may qualify for our Innovator's Reward program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDEL1
Entrez