FEZ1 Antibody

Images

 
Western Blot: FEZ1 Antibody [NB100-53816] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: FEZ1 Antibody [NB100-53816] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FEZ1 Antibody Summary

Immunogen
Peptide with sequence C-QNKQKEQRELMKK corresponding to internal region according to NP_005094.1.
Specificity
This antibody is expected to recognize isoform 1 (NP_005094.1) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FEZ1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.01 - 0.03 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 45 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 45.1 kDa band according to NP_005094.1). IHC: Paraffin embedded Human Colon and Brain (Cortex).
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FEZ1 Lysate (NBL1-10679)
Brain Frontal Lobe Lysate (NB820-59186)
Publications
Read Publications using NB100-53816.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FEZ1 Antibody

  • fasciculation and elongation protein zeta 1 (zygin I)
  • fasciculation and elongation protein zeta-1
  • Zygin I
  • zygin-1

Background

This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FEZ1 Antibody (NB100-53816)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FEZ1
Entrez
Uniprot