GSK-3 beta Antibody (3D10) - BSA Free

Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas and GSK-3 beta in a murine xenograft model. SW620 cells injected into flanks of athymic Nude mice & tumor volume was checked biweekly. Mice treated weekly with oxaliplatin (days 0, 7 and 14) & injected daily with Fap1 blocking SLV peptide, VLS control peptide, or treated with SLV or VLS peptide alone (n=12). Tumors were simultaneously harvested when control tumors were >2,000 mm3. SLV peptide increases Gsk3-phosphorylation with/without oxaliplatin. IF detection of total versus phospho- GSK-3 beta performed with DAPI staining of nuclei (areas without gland formation were selected). Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.25401), licensed under a CC-BY licence.

Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.

Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).

Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
• Clone: 3D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

GSK-3 beta Antibody (3D10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: GSK3B
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Simple Western 1:50
  • Western Blot 1:500-1:2000
• Application Notes: This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  K-562 Whole Cell Lysate
  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for GSK-3 beta Antibody (3D10) - BSA Free

• EC 2.7.11
• EC 2.7.11.26
• glycogen synthase kinase 3 beta
• glycogen synthase kinase-3 beta
• GSK3 beta
• GSK-3 beta
• GSK3B
• GSK3beta isoform

Background

GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GSK-3 beta Antibody (NBP1-47470)(8)

Publications using NBP1-47470

• Feng C, Chen Y, Zhang Y et al. PTEN regulates mitochondrial biogenesis via the AKT/GSK-3 beta/PGC-1 alpha pathway in autism Neuroscience 2021-04-22 [PMID: 33895342]
• Purvis N, Kumari S, Chandrasekera D et al. Diabetes induces dysregulation of microRNAs associated with survival, proliferation and self-renewal in cardiac progenitor cells Diabetologia 2021-03-02 [PMID: 33655378] (WB, Mouse)
• Huang W, Bei L, Eklund EA. Inhibition of Fas associated phosphatase 1 (Fap1) facilitates apoptosis of colon cancer stem cells and enhances the effects of oxaliplatin Oncotarget 2018-05-25 [PMID: 29899829] (IHC-P, Human)
• Dey S, Goswami S, EiSa A et al. Cyclic AMP and glycogen synthase kinase 3 form a regulatory loop in spermatozoa J. Cell. Physiol. 2018-03-25 [PMID: 29574946] (WB, Mouse)
• Marathe S, Liu S, Brai E et al. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry Cell Death Differ. 2015-03-27 [PMID: 25822340] (ICC/IF, IHC, Mouse)
• Xu R, Hu Q, Ma Q et al. The protease Omi regulates mitochondrial biogenesis through the GSK3B/PGC-1a pathway Cell Death Dis 2014-08-14 [PMID: 25118933]
• Cosgun T, Kisacik O Determination of Nurses' Attitudes towards Nutritional Assessment, Level of Knowledge on Nutritional Care, and Perceived Quality of Care Celal Bayar Universitesi Saglık Bilimleri Enstitusu Dergisi 2021-06-30
• Lamichhane S, Mo JS, Sharma G et al. MIR133A regulates cell proliferation, migration, and apoptosis by targeting SOX9 in human colorectal cancer cells American journal of cancer research 2022-07-15 [PMID: 35968353] (WB, Mouse)

Reviews for GSK-3 beta Antibody (NBP1-47470) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer WB Human 11/30/2016

Summary

• Application: Western Blot
• Sample Tested: MM1S (multiple myeloma cell line)
• Species: Human
• Lot: CCWHO11601A

Comments

• Comments: Conditions:
  - MM1S cells lysed with NP40 lysis buffer
  -protein quantified with BCA assay, 10 ug total protein loaded per lane
  -protein resolved by SDS-PAGE on 7.5% gel
  -transferred onto PVDF membrane and blocked with 5% BSA in TBST for 2hrs at room temperature
  -incubated with antibody (1:10,000) in TBST (+1% BSA) overnight at 4 deg with shaking
  -secondary incubation with HRP-conjugated anti-mouse antibody (1:10,000) for 1hr at room temperature
  -visualized with ECL chemilumnescence, imaged with film, exposure for 2 mins

reviewed by: Jason Spaeth WB Mouse 11/10/2016

Summary

• Application: Western Blot
• Sample Tested: mouse beta cell line
• Species: Mouse
• Lot: N/A

reviewed by: Anonymous - Simple Western Mouse 08/05/2015

Summary

• Application: Simple Western
• Species: Mouse

reviewed by: Verified Customer Simple Western Human 03/24/2015

Summary

• Application: Simple Western
• Sample Tested: Human mesenchymal stem cell whole cell lysate
• Species: Human
• Lot: A-1

FAQs for GSK-3 beta Antibody (NBP1-47470). (Showing 1 - 2 of 2 FAQs).

1. I received this antibody in February 2012 and stored the unopened package immediately at 4C. Today (4/5/2012), I opened the package, placed the vial and centrifuged it briefly. I opened the top and found some thick beige-colored liquid which measured around 50 ul. I don't think I can use the antibody. Could you help me with the problem, please?
   • NBP1-47470 is supplied as ascitic fluid, which frequently has a beige-like color, and this could be what you're seeing in your vial. Otherwise, I'm concerned that the antibody may have been damaged by storing it a 4 C for such a long time. There is a very low concentration of preservative in this antibody, which is why we recommend storage at -20 C long term.
2. Our customer would like to know whether NBP1-47470, GSK3 beta Antibody can be used in IF. It shows on the website that this antibody can be used to detect the target protein through ICC/IF. But his sampels are paraffin-embedded tissues, no cultured cells. Thus he is not sure. Please give us some advice and help.
   • Our GSK3 beta antibody with catalogue number NBP1-47470 has been validated for IHC with paraffin-embedded tissues, and has also been validated for immunocytochemistry. Our immunocytochemistry data can be seen in image 3 on our website, whilst our IHC-P data can be seen in image 4. This antibody is covered by our 100% quality guarantee for your customer's application of IHC with paraffin-embedded tissues. Currently tested species are human, mouse, rat and primate.

Bioinformatics

• Gene Symbol: GSK3B
• Entrez:
  • 2932(Human)
• Uniprot:
  • P49841()