Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin ...read more
Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.
Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).
Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.
Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor ...read more
This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
46 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
Ammonium sulfate precipitation
Alternate Names for GSK-3 beta Antibody (3D10)
glycogen synthase kinase 3 beta
glycogen synthase kinase-3 beta
GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Conditions: - MM1S cells lysed with NP40 lysis buffer -protein quantified with BCA assay, 10 ug total protein loaded per lane -protein resolved by SDS-PAGE on 7.5% gel -transferred onto PVDF membrane and blocked with 5% BSA in TBST for 2hrs at room temperature -incubated with antibody (1:10,000) in TBST (+1% BSA) overnight at 4 deg with shaking -secondary incubation with HRP-conjugated anti-mouse antibody (1:10,000) for 1hr at room temperature -visualized with ECL chemilumnescence, imaged with film, exposure for 2 mins
FAQs for GSK-3 beta Antibody (NBP1-47470). (Showing 1 - 2 of 2 FAQs).
I received this antibody in February 2012 and stored the unopened package immediately at 4C. Today (4/5/2012), I opened the package, placed the vial and centrifuged it briefly. I opened the top and found some thick beige-colored liquid which measured around 50 ul. I don't think I can use the antibody. Could you help me with the problem, please?
NBP1-47470 is supplied as ascitic fluid, which frequently has a beige-like color, and this could be what you're seeing in your vial. Otherwise, I'm concerned that the antibody may have been damaged by storing it a 4 C for such a long time. There is a very low concentration of preservative in this antibody, which is why we recommend storage at -20 C long term.
Our customer would like to know whether NBP1-47470, GSK3 beta Antibody can be used in IF. It shows on the website that this antibody can be used to detect the target protein through ICC/IF. But his sampels are paraffin-embedded tissues, no cultured cells. Thus he is not sure. Please give us some advice and help.
Our GSK3 beta antibody with catalogue number NBP1-47470 has been validated for IHC with paraffin-embedded tissues, and has also been validated for immunocytochemistry. Our immunocytochemistry data can be seen in image 3 on our website, whilst our IHC-P data can be seen in image 4. This antibody is covered by our 100% quality guarantee for your customer's application of IHC with paraffin-embedded tissues. Currently tested species are human, mouse, rat and primate.
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