GSK-3 beta Antibody (3D10)

Images

 
Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin ...read more
Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.
Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed ...read more
Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.
Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).
Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GSK-3 beta Antibody (3D10) Summary

Immunogen
Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GSK3B
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:50
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
K-562 Whole Cell Lysate
NIH 3T3 Whole Cell Lysate
GSK-3 beta Knockout HeLa Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4.8
using
NBP1-47470 in the following applications:

Publications
Read Publications using
NBP1-47470 in the following applications:

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for GSK-3 beta Antibody (3D10)

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GSK-3 beta Antibody (NBP1-47470)(4)

Reviews for GSK-3 beta Antibody (NBP1-47470) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-47470:
Filter by Applications
WB
(2)
Simple Western
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot GSK-3 beta NBP1-47470
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reviewed by:
Natalie Erdmann
WB Human 11/30/2016
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Summary

ApplicationWestern Blot
Sample TestedMM1S (multiple myeloma cell line)
SpeciesHuman
LotCCWHO11601A

Comments

CommentsConditions:
- MM1S cells lysed with NP40 lysis buffer
-protein quantified with BCA assay, 10 ug total protein loaded per lane
-protein resolved by SDS-PAGE on 7.5% gel
-transferred onto PVDF membrane and blocked with 5% BSA in TBST for 2hrs at room temperature
-incubated with antibody (1:10,000) in TBST (+1% BSA) overnight at 4 deg with shaking
-secondary incubation with HRP-conjugated anti-mouse antibody (1:10,000) for 1hr at room temperature
-visualized with ECL chemilumnescence, imaged with film, exposure for 2 mins
Western Blot GSK-3 beta NBP1-47470
Enlarge
reviewed by:
Anonymous
WB Mouse 11/10/2016
View

Summary

ApplicationWestern Blot
Sample Testedmouse beta cell line
SpeciesMouse
LotN/A
Simple Western GSK-3 beta NBP1-47470
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reviewed by:
Anonymous
Simple Western Mouse 08/05/2015
View

Summary

ApplicationSimple Western
SpeciesMouse
Special ApplicationsImage courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.
FileView PDF
Simple Western GSK-3 beta NBP1-47470
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reviewed by:
Zachary Patinkin
Simple Western Human 03/24/2015
View

Summary

ApplicationSimple Western
Sample TestedHuman mesenchymal stem cell whole cell lysate
SpeciesHuman
LotA-1

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio1:50, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GSK-3 beta Antibody (NBP1-47470). (Showing 1 - 2 of 2 FAQs).

  1. I received this antibody in February 2012 and stored the unopened package immediately at 4C. Today (4/5/2012), I opened the package, placed the vial and centrifuged it briefly. I opened the top and found some thick beige-colored liquid which measured around 50 ul. I don't think I can use the antibody. Could you help me with the problem, please?
    • NBP1-47470 is supplied as ascitic fluid, which frequently has a beige-like color, and this could be what you're seeing in your vial. Otherwise, I'm concerned that the antibody may have been damaged by storing it a 4 C for such a long time. There is a very low concentration of preservative in this antibody, which is why we recommend storage at -20 C long term.
  2. Our customer would like to know whether NBP1-47470, GSK3 beta Antibody can be used in IF. It shows on the website that this antibody can be used to detect the target protein through ICC/IF. But his sampels are paraffin-embedded tissues, no cultured cells. Thus he is not sure. Please give us some advice and help.
    • Our GSK3 beta antibody with catalogue number NBP1-47470 has been validated for IHC with paraffin-embedded tissues, and has also been validated for immunocytochemistry. Our immunocytochemistry data can be seen in image 3 on our website, whilst our IHC-P data can be seen in image 4. This antibody is covered by our 100% quality guarantee for your customer's application of IHC with paraffin-embedded tissues. Currently tested species are human, mouse, rat and primate.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-47470AF350
Alexa Fluor 405 NBP1-47470AF405
Alexa Fluor 488 NBP1-47470AF488
Alexa Fluor 532 NBP1-47470AF532
Alexa Fluor 594 NBP1-47470AF594
Alexa Fluor 647 NBP1-47470AF647
Alexa Fluor 700 NBP1-47470AF700
Alexa Fluor 750 NBP1-47470AF750
Allophycocyanin NBP1-47470APC
Allophycocyanin/Cy7 NBP1-47470APCCY7
Biotin NBP1-47470B
DyLight 350 NBP1-47470UV
DyLight 405 NBP1-47470V
DyLight 488 NBP1-47470G
DyLight 550 NBP1-47470R
DyLight 594 NBP1-47470DL594
DyLight 650 NBP1-47470C
DyLight 680 NBP1-47470FR
DyLight 755 NBP1-47470IR
FITC NBP1-47470F
HRP NBP1-47470H
Janelia Fluor 549 NBP1-47470JF549
Janelia Fluor 646 NBP1-47470JF646
PE NBP1-47470PE
PE/Atto594 NBP1-47470PEATT594
PE/Cy5.5 NBP1-47470PECY55
PE/Cy7 NBP1-47470PECY7
PerCP NBP1-47470PCP

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Natalie Erdmann
11/30/2016
Application: WB
Species: Human

Anonymous
11/10/2016
Application: WB
Species: Mouse

Anonymous
08/05/2015
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol GSK3B
Entrez
Uniprot
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