DIO3 Antibody - BSA Free


Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.
Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.

Product Details

Reactivity Hu, Mu, Rt, Ha, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
BSA Free
1 mg/ml

Order Details

DIO3 Antibody - BSA Free Summary

Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]
Cell membrane; Single-pass type II membrane protein
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry reported by customer review using biotinylated antibody
  • Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 24885572)
  • Immunohistochemistry reported in scientific literature (PMID 24174657)
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 24885572)
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 25004090)
  • Western Blot 0.5 ug/ml
Application Notes
In Western blot, a band is seen ~31 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DIO3 Peptide (NBP1-05767PEP)
Reviewed Applications
Read 2 Reviews rated 3.5
NBP1-05767 in the following applications:

Read Publications using
NBP1-05767 in the following applications:

Reactivity Notes

Rabbit reactivity via customer review.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for DIO3 Antibody - BSA Free

  • 5DIII
  • D3
  • deiodinase, iodothyronine, type III
  • EC 1.97.1
  • EC
  • ITDI3
  • placental type
  • thyroxine deiodinase type III (selenoprotein)
  • Type 3 DI
  • type 3 iodothyronine selenodeiodinase
  • type III iodothyronine deiodinase
  • type-III 5' deiodinase
  • Type-III 5'-deiodinase


DIO3 (type III iodothyronine deiodinase), an iodothyronine deiodinase family member, is expressed in placenta and several fetal tissues in the plasma/endosomal membranes as single-pass type II membrane protein, and it catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine), both RT3 and T2 being inactive metabolites thus preventing premature exposure of developing fetus to adult levels of thyroid hormones. DIO3 controls circulating thyroid hormone levels throughout gestation by controlling thyroid hormone inactivation during embryological development. DIO3 is homodimeric and may undergo minor heretodimerization with DIO1 or DIO2, homo- or heterodimer state being critical for its catalytic activity. Its activity has been identified in only a limited number of postnatal tissues, i.e. brain, skin, and pregnant uterus, whereas it is abundantly present in several fetal tissues. Furthermore, DIO3 re-expression has been shown in certain pathologies that include cancer, cardiac hypertrophy, myocardial infarction, chronic inflammation, and critical illness.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Sh
Applications: Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu
Applications: Flow-IC, Flow, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: DB, ELISA, ICC/IF, IP, WB
Species: Hu
Applications: IHC, WB
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Bv, Fe, Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Pm, Pm
Applications: IHC, IHC-P
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ELISA, AP, PA, WB
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
Species: Av, Hu, Mu, Rt
Applications: ELISA, IHC, WB
Species: Hu, Mu, Rt, Ha, Rb
Applications: WB, Flow, ICC/IF, IHC

Publications for DIO3 Antibody (NBP1-05767)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, PLA, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NBP1-05767 Applications Species
Peluso T, Nittoli V, Reale C et al. Chronic Exposure to Chlorpyrifos Damages Thyroid Activity and Imbalances Hepatic Thyroid Hormones Signaling and Glucose Metabolism: Dependency of T3-FOXO1 Axis by Hyperglycemia International journal of molecular sciences 2023-05-31 [PMID: 37298533] (WB, Mouse)

Dilution: 1:3000
WB Mouse
Kohon MY, Zaaroor Levy M, Hornik-Lurie T et al. ?v?3 Integrin as a Link between the Development of Fibrosis and Thyroid Hormones in Systemic Sclerosis International journal of molecular sciences 2023-05-18 [PMID: 37240272] (WB, Human) WB Human
Salas-Lucia F, Fekete C, Sinkó R et al. Axonal T3 uptake and transport can trigger thyroid hormone signaling in the brain eLife 2023-05-19 [PMID: 37204837] (ICC/IF, IHC, Mouse, Rat) ICC/IF, IHC Mouse, Rat
Agarwal S, Koh KH, Tardi NJ Et al. Deiodinase-3 is a thyrostat to regulate podocyte homeostasis EBioMedicine 2021-10-01 [PMID: 34649077] (WB, ICC/IF, PLA, Mouse, Human) WB, ICC/IF, PLA Mouse, Human
Devalraju Kp, Tripathi D, Neela Vsk Et Al. Reduced thyroxine production in young household contacts of tuberculosis patients increases active tuberculosis disease risk JCI insight 2021-07-08 [PMID: 34236051] (WB) WB
Benedetti V, Lavecchia AM, Locatelli M et al. Alteration of thyroid hormone signaling triggers the diabetes-induced pathological growth, remodeling, and dedifferentiation of podocytes JCI Insight 2019-09-19 [PMID: 31534055] (IHC-P, Rat) IHC-P Rat
Porings AS, Lowin T, Dufner B et al. A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients Sci Rep [PMID: 31519956] (IHC-Fr, Human) IHC-Fr Human
Calonne J, Isacco L, Miles-Chan J et al. Reduced Skeletal Muscle Protein Turnover and Thyroid Hormone Metabolism in Adaptive Thermogenesis That Facilitates Body Fat Recovery During Weight Regain Front Endocrinol (Lausanne) 2019-02-28 [PMID: 30873123] (WB, Rat) WB Rat
Yamauchi I, Sakane Y, Yamashita T et al. Effects of growth hormone on thyroid function are mediated by type 2 iodothyronine deiodinase in humans. Endocrine 2017-12-22 [PMID: 29274063] (WB, Human) WB Human
De Andrade PB, Neff LA, Strosova MK et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol 2015-01-01 [PMID: 26441673]
Show All 17 Publications.

Reviews for DIO3 Antibody (NBP1-05767) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NBP1-05767:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Western Blot DIO3 NBP1-05767
reviewed by:
Verified Customer
WB Other 01/28/2013


ApplicationWestern Blot
Sample Testedbrain whole cell homogenate
reviewed by:
System Admin
WB Human 01/05/2010


ApplicationWestern Blot
Sample TestedHEK293 cells transfected with human D3 expression vector

Product General Protocols

View specific protocols for DIO3 Antibody (NBP1-05767): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DIO3 Antibody (NBP1-05767). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for a Dio3 peptide that I can inject in vivo to produce a biological effect. Can you provide information or confirm whether the Dio3 peptide you have would be effective as a biological agent in vivo?
    • I have pulled up information on the Dio3 peptides and recombinant proteins that we have available. Unfortunately I would not expect any of them to be biologically active and capable of producing a response when injected in vivo. The two peptides we carry are simply intended to be used for negative control competition assays to block signal of the primary and determine what is specific and non-specific as far as signal is concerned. The other two recombinant proteins are ones we distribute for a Taiwanese company called Abnova and they should not be biologically active based on the cell free wheat germ system employed to synthesize them. We will typically list our active proteins with an indication of such on our datasheet if it has been tested and usually the application of functional listed.
  2. We have used one of your antibodies against Dio3 (cat# NBP1-05767) in the past and, despite it being directed against rodent D3 peptide sequence, it also appears to stains human D3 specifically in our hands. Can you please let us know the rodent D3 peptide sequence that was used to create this antibody? Alternatively, can you let us know if this rodent D3 peptide sequence also matches the human D3 peptide sequence? If there is a high degree of sequence homology, we wish to publish our findings and include a description that your antibody works in human tissues (IHC).We also noticed a new D3 aB on your website (cat# NBP1-19747) that is said to recognize human D3. Can you also let me know the sequence of the peptide used to generate it? We are one of the only labs in the world that study D3. If you can send us some samples of these antibodies, we can likely help you validate them experimentally for Western, IHC, etc.
    • The immunogen shares 93% homology to human. It is expected to cross-react with human cells. Furthermore, full-length DIO3 in human and mouse share greater than 95% similarity over the entire protein. It will be very difficult to find an antibody that works on one species but not the other unless the immunogen falls within amino acids 48-55, which is the only region where there is significant difference between the two species. It is highly doubtful you will find a commercial antibody that uses this immunogen to generate their antibody as most companies will want to increase the amount of species recognized by an antibody, not decrease it. For NBP1-19747, like all of our immunogens, the exact sequence is considered proprietary, but as you can see on our datasheet, the immunogen surrounds amino acid 66, which does not fall within the range where mouse and human are dissimilar.

Secondary Antibodies


Isotype Controls

Additional DIO3 Products

Array NBP1-05767

Bioinformatics Tool for DIO3 Antibody (NBP1-05767)

Discover related pathways, diseases and genes to DIO3 Antibody (NBP1-05767). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DIO3 Antibody (NBP1-05767)

Discover more about diseases related to DIO3 Antibody (NBP1-05767).

Pathways for DIO3 Antibody (NBP1-05767)

View related products by pathway.

PTMs for DIO3 Antibody (NBP1-05767)

Learn more about PTMs related to DIO3 Antibody (NBP1-05767).

Research Areas for DIO3 Antibody (NBP1-05767)

Find related products by research area.

Blogs on DIO3

There are no specific blogs for DIO3, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Verified Customer
Application: WB
Species: Other

System Admin
Application: WB
Species: Human


Gene Symbol DIO3