DIO3 Antibody


Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.
Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.

Product Details

Reactivity Hu, Mu, Rt, Ha, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1 mg/ml

DIO3 Antibody Summary

Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]
Cell membrane; Single-pass type II membrane protein
Immunogen affinity purified
Innovator's Reward
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  • Western Blot 0.5 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This DIO3 antibody is useful for Western blot, where a band is seen ~31 kDa. Flow data from customer review using biotinylated antibody. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25004090). Use in Immunohistochemistry-Frozen and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24885572). Use in Immunohistochemistry reported in scientific literature (PMID: 24174657).The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Rat Placenta Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
DIO3 Peptide (NBP1-05767PEP)
Reviewed Applications
Read 2 Reviews rated 3.5
NBP1-05767 in the following applications:

Read Publications using
NBP1-05767 in the following applications:

Reactivity Notes

Rabbit reactivity via customer review.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for DIO3 Antibody

  • 5DIII
  • D3
  • deiodinase, iodothyronine, type III
  • EC 1.97.1
  • EC
  • ITDI3
  • placental type
  • thyroxine deiodinase type III (selenoprotein)
  • Type 3 DI
  • type 3 iodothyronine selenodeiodinase
  • type III iodothyronine deiodinase
  • type-III 5' deiodinase
  • Type-III 5'-deiodinase


DIO3 (type III iodothyronine deiodinase), an iodothyronine deiodinase family member, is expressed in placenta and several fetal tissues in the plasma/endosomal membranes as single-pass type II membrane protein, and it catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine), both RT3 and T2 being inactive metabolites thus preventing premature exposure of developing fetus to adult levels of thyroid hormones. DIO3 controls circulating thyroid hormone levels throughout gestation by controlling thyroid hormone inactivation during embryological development. DIO3 is homodimeric and may undergo minor heretodimerization with DIO1 or DIO2, homo- or heterodimer state being critical for its catalytic activity. Its activity has been identified in only a limited number of postnatal tissues, i.e. brain, skin, and pregnant uterus, whereas it is abundantly present in several fetal tissues. Furthermore, DIO3 re-expression has been shown in certain pathologies that include cancer, cardiac hypertrophy, myocardial infarction, chronic inflammation, and critical illness.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DIO3 Antibody (NBP1-05767)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Hamster.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-05767 Applications Species
Benedetti V, Lavecchia AM, Locatelli M et al. Alteration of thyroid hormone signaling triggers the diabetes-induced pathological growth, remodeling, and dedifferentiation of podocytes JCI Insight Sep 19 2019 [PMID: 31534055] (IHC-P, Rat) IHC-P Rat
Porings AS, Lowin T, Dufner B et al. A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients Sci Rep [PMID: 31519956] (IHC-Fr, Human) IHC-Fr Human
Calonne J, Isacco L, Miles-Chan J et al. Reduced Skeletal Muscle Protein Turnover and Thyroid Hormone Metabolism in Adaptive Thermogenesis That Facilitates Body Fat Recovery During Weight Regain Front Endocrinol (Lausanne) Feb 28 2019 [PMID: 30873123] (WB, Rat) WB Rat
Yamauchi I, Sakane Y, Yamashita T et al. Effects of growth hormone on thyroid function are mediated by type 2 iodothyronine deiodinase in humans. Endocrine 2017 Dec 22 [PMID: 29274063] (WB, Human) WB Human
De Andrade PB, Neff LA, Strosova MK et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol 2015 [PMID: 26441673]
Castroneves LA, Jugo RH, Maynard MA et al. Mice with hepatocyte-specific deficiency of type 3 deiodinase have intact liver regeneration and accelerated recovery from nonthyroidal illness after toxin-induced hepatonecrosis. Endocrinology 2014 Jul 08 [PMID: 25004090] (IHC-P, Mouse)

DIO3 antibody used at 1:2000 dilution for IHC-P alongwith isotype negative controls on wild-type D3 /D3flox allele and LD3KO/recombined D3null allele mice treated with CCl4. DIO3 positivity was seen in hepatocytes of D3-flox but not in LD3KO mice (Figure 2D).
IHC-P Mouse
Medina MC, Foseca T, Molina J et al. Maternal Inheritance of an Inactive Type III Deiodinase Gene Allele Affects Mouse Pancreatic b-cells and Disrupts Glucose Homeostasis Endocrinology. 2014 Jun 02 [PMID: 24885572] (IHC-Fr, ICC/IF, Mouse)

Antibody used for IHC-Fr on wild type (WT), inherited paternally/maternally (Htz-P/Htz-P) or D3 knockout (mouse with the disruption of Dio3 gene/D3KO) mouse pancreata - fixed in 4% PFA ON 4C, cryoprotected in 30% sucrose ON 4C, 8 um sections cut and immunostained with primary followed by Alexa conjugated secondary (Figure 2A and Figure 3A). D3 control peptide (NBP1-05767PEP) used as a negative control and no staining observed in antibody preincubated with the peptide. Antibody also used for ICC-IF in MIN6-C3 cells (Supplemental Figure 3)
IHC-Fr, ICC/IF Mouse
Maynard MA, Marino-Enriquez A, Fletcher JA et al. Thyroid hormone inactivation in gastrointestinal stromal tumors. N Engl J Med 2014 Apr 03 [PMID: 24693892] (IHC-P, Human)

Using the Biotin conjugated version of NBP1-05767, catalog number NBP1-05767B.
IHC-P Human
Vose LR, Vinukonda G, Jo S et al. Treatment with thyroxine restores myelination and clinical recovery after intraventricular hemorrhage. J Neurosci. 2013 Oct 30 [PMID: 24174657] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Jo S, Kallo I, Bardoczi Z, Arrojo E et al. Neuronal Hypoxia Induces Hsp40-Mediated Nuclear Import of Type 3 Deiodinase As an Adaptive Mechanism to Reduce Cellular Metabolism J Neurosci 2012 Jun [PMID: 22723689] (WB, Human, Hamster) WB Human, Hamster
Show All 12 Publications.

Reviews for DIO3 Antibody (NBP1-05767) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using NBP1-05767:
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Images Ratings Applications Species Date Details
Western Blot DIO3 NBP1-05767
reviewed by:
Linnea Vose
WB Other 01/28/2013


ApplicationWestern Blot
Sample Testedbrain whole cell homogenate
CommentsWe often get a nonspecific band ~50kD with our rabbit samples. There appears to be three rabbit bands approx. the size of Dio3, but the bottom band is brightest, and corresponds with the positive control.


Blocking Details5% BSA in TBST, 1h 37C

Primary Anitbody

Dilution Ratio1:2000 in 2.5% BSA in TBST, overnight at 4C

Secondary Antibody

Secondary DescriptionJackson Goat anti Rabbit HRP


Detection NotesECL, radiography film, 15s exposure


CommentsWe often get a nonspecific band ~50kD with our rabbit samples. There appears to be three rabbit bands approx. the size of Dio3, but the bottom band is brightest, and corresponds with the positive control.
reviewed by:
WB Human 01/05/2010


ApplicationWestern Blot
Sample TestedHEK293 cells transfected with human D3 expression vector


Blocking DetailsBlocking Buffer: 1% Roche Blocking solution, Blocking Time: overnight, Blocking Temp: 4 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1/2000, Incubation Dilution Buffer: 0.5% Roche blocking, Incubation Time: 2 hr, Incubation Temp: 25C

Secondary Antibody

Secondary DescriptionSecondary Ab Host Species: Roche anti-rabbit, Secondary Ab Dilution Ratio: 1/5000


Detection NotesPierce, 1min Exposure, Pos Ctrl:transfected cells, Neg Ctrl:untransfected cells, Bands: Specific=~30kDa, Non-Specific=many

Product General Protocols

View specific protocols for DIO3 Antibody (NBP1-05767): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DIO3 Antibody (NBP1-05767). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for a Dio3 peptide that I can inject in vivo to produce a biological effect. Can you provide information or confirm whether the Dio3 peptide you have would be effective as a biological agent in vivo?
    • I have pulled up information on the Dio3 peptides and recombinant proteins that we have available. Unfortunately I would not expect any of them to be biologically active and capable of producing a response when injected in vivo. The two peptides we carry are simply intended to be used for negative control competition assays to block signal of the primary and determine what is specific and non-specific as far as signal is concerned. The other two recombinant proteins are ones we distribute for a Taiwanese company called Abnova and they should not be biologically active based on the cell free wheat germ system employed to synthesize them. We will typically list our active proteins with an indication of such on our datasheet if it has been tested and usually the application of functional listed.
  2. We have used one of your antibodies against Dio3 (cat# NBP1-05767) in the past and, despite it being directed against rodent D3 peptide sequence, it also appears to stains human D3 specifically in our hands. Can you please let us know the rodent D3 peptide sequence that was used to create this antibody? Alternatively, can you let us know if this rodent D3 peptide sequence also matches the human D3 peptide sequence? If there is a high degree of sequence homology, we wish to publish our findings and include a description that your antibody works in human tissues (IHC).We also noticed a new D3 aB on your website (cat# NBP1-19747) that is said to recognize human D3. Can you also let me know the sequence of the peptide used to generate it? We are one of the only labs in the world that study D3. If you can send us some samples of these antibodies, we can likely help you validate them experimentally for Western, IHC, etc.
    • The immunogen shares 93% homology to human. It is expected to cross-react with human cells. Furthermore, full-length DIO3 in human and mouse share greater than 95% similarity over the entire protein. It will be very difficult to find an antibody that works on one species but not the other unless the immunogen falls within amino acids 48-55, which is the only region where there is significant difference between the two species. It is highly doubtful you will find a commercial antibody that uses this immunogen to generate their antibody as most companies will want to increase the amount of species recognized by an antibody, not decrease it. For NBP1-19747, like all of our immunogens, the exact sequence is considered proprietary, but as you can see on our datasheet, the immunogen surrounds amino acid 66, which does not fall within the range where mouse and human are dissimilar.

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-05767AF350
Alexa Fluor 405 NBP1-05767AF405
Alexa Fluor 488 NBP1-05767AF488
Alexa Fluor 532 NBP1-05767AF532
Alexa Fluor 594 NBP1-05767AF594
Alexa Fluor 647 NBP1-05767AF647
Alexa Fluor 700 NBP1-05767AF700
Alexa Fluor 750 NBP1-05767AF750
Biotin NBP1-05767B
DyLight 350 NBP1-05767UV
DyLight 405 NBP1-05767V
DyLight 488 NBP1-05767G
DyLight 550 NBP1-05767R
DyLight 594 NBP1-05767DL594
DyLight 650 NBP1-05767C
DyLight 680 NBP1-05767FR
DyLight 755 NBP1-05767IR
FITC NBP1-05767F
HRP NBP1-05767H
Janelia Fluor 549 NBP1-05767JF549
Janelia Fluor 646 NBP1-05767JF646

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Array NBP1-05767

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Recent Reviews


Linnea Vose
Application: WB
Species: Other

Application: WB
Species: Human


Gene Symbol DIO3