Dopamine Receptor D4 Antibody (HL2851)

Unboiled Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D4 antibody [HL2851] (NBP3-48739) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D4 antibody [HL2851] (NBP3-48739) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Dopamine Receptor D4 antibody [HL2851] detects Dopamine Receptor D4 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human stomach tumor.Dopamine Receptor D4 stained by Dopamine Receptor D4 antibody [HL2851] (NBP3-48739) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Dopamine Receptor D4 antibody [HL2851] detects Dopamine Receptor D4 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: Dopamine Receptor D4 stained by Dopamine Receptor D4 antibody [HL2851] (NBP3-48739) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: HL2851
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dopamine Receptor D4 Antibody (HL2851) Summary

• Description: Novus Biologicals Rabbit Dopamine Receptor D4 Antibody (HL2851) (NBP3-48739) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide encompassing a sequence within the Extracellular domain human of Dopamine Receptor D4. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DRD4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Dopamine Receptor D4 Antibody (HL2851)

• D(2C) dopamine receptor
• D(4) dopamine receptor
• D4DR
• Dopamine D4 receptor
• Dopamine Receptor D4
• DRD4
• seven transmembrane helix receptor

Background

DRD4, a Dopamine Receptor, has been suggested to affect personality, substance dependence, cognition, and emotion. DRD4 binds the antipsychotic medications clozapine and spiperone. DRD4 knock-out mice are anatomically normal but display altered locomotion and sensitivity to drugs such as clozapine, cocaine, and others. The DRD4 gene contains extensive polymorphisms consisting of variable numbers of tandem repeats (VNTR) in sequences corresponding to the third cytoplasmic loop of the receptor; at least 27 different haplotypes encoding 20 different protein variants have been documented. The frequency of the different variants varies among population groups. Studies have suggested a link between VNTR and human personality traits (such as novelty seeking) and diseases (such as schizophrenia, bipolar affective disorder, and Tourette syndrome); however, considerable debates exist regarding the validity and significance of such associations. DRD4 expression has been reported in various regions of the brain as well as in adrenal gland, artery, eye, heart, kidney, placenta, spinal cord, testis, and vas deferens. ESTs have been isolated from a kidney library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DRD4