Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for ...read more
Immunohistochemistry: DDX17 Antibody [NB200-352] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate (50 ug) from NIH3T3, TCMK1,4T1,CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody
Biological Strategies: Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), MCF7 (M; 50 ug) and K562 (K; 50 ug) cells. Antibody used at 0.1 ug/ml.
Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352] - FFPE section of human seminoma. Affinity purified rabbit anti-DDX17 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory ...read more
The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 613) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 using the numbering given in entry AAC50787.1 (GeneID 10521).
Immunogen affinity purified
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Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
DDX17 (p72) is a highly related member of the DEAD box family and is an established RNA helicase. It has been implicated in growth regulation and has been shown to be involved in both pre-mRNA and pre-rRNA processing. This protein has also been reported to act as a transcriptional co-activator for estrogen-receptor alpha (ERa) and shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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