DDX17 Antibody

Images

 
Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for ...read more
Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate (50 ug) from NIH3T3, TCMK1,4T1,CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

DDX17 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 613) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 using the numbering given in entry AAC50787.1 (GeneID 10521).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX17
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:15000
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500 -1:2000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
DDX17 Overexpression Lysate
DDX17 Knockout 293T Cell Lysate
Publications
Read Publications using
NB200-352 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX17 Antibody

  • DDX17
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
  • DEAD box protein 17
  • DEAD box protein p72
  • DEAD/H box 17
  • DKFZp761H2016
  • EC 3.6.1
  • EC 3.6.4.13
  • p72
  • P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
  • probable ATP-dependent RNA helicase DDX17
  • RH70
  • RNA-dependent helicase p72

Background

DDX17 (p72) is a highly related member of the DEAD box family and is an established RNA helicase. It has been implicated in growth regulation and has been shown to be involved in both pre-mRNA and pre-rRNA processing. This protein has also been reported to act as a transcriptional co-activator for estrogen-receptor alpha (ERa) and shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX17 Antibody (NB200-352)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DDX17 Antibody (NB200-352) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-352AF350
Alexa Fluor 405 NB200-352AF405
Alexa Fluor 488 NB200-352AF488
Alexa Fluor 532 NB200-352AF532
Alexa Fluor 594 NB200-352AF594
Alexa Fluor 647 NB200-352AF647
Alexa Fluor 700 NB200-352AF700
Alexa Fluor 750 NB200-352AF750
Biotin NB200-352B
DyLight 350 NB200-352UV
DyLight 405 NB200-352V
DyLight 488 NB200-352G
DyLight 550 NB200-352R
DyLight 594 NB200-352DL594
DyLight 650 NB200-352C
DyLight 680 NB200-352FR
DyLight 755 NB200-352IR
FITC NB200-352F
HRP NB200-352H
Janelia Fluor 549 NB200-352JF549
Janelia Fluor 646 NB200-352JF646

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Bioinformatics

Gene Symbol DDX17
Entrez
Uniprot