DDX17 Antibody - BSA Free

Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate (50 ug) from NIH3T3, TCMK1,4T1,CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DDX17 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 613) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 using the numbering given in entry AAC50787.1 (GeneID 10521).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX17
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 -1:2000
  • Immunohistochemistry-Paraffin 1:500 -1:2000
  • Western Blot 1:1000 - 1:15000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX17 Antibody - BSA Free

• DDX17
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• DEAD box protein 17
• DEAD box protein p72
• DEAD/H box 17
• DKFZp761H2016
• EC 3.6.1
• EC 3.6.4.13
• p72
• P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
• probable ATP-dependent RNA helicase DDX17
• RH70
• RNA-dependent helicase p72

Background

DDX17 (p72) is a highly related member of the DEAD box family and is an established RNA helicase. It has been implicated in growth regulation and has been shown to be involved in both pre-mRNA and pre-rRNA processing. This protein has also been reported to act as a transcriptional co-activator for estrogen-receptor alpha (ERa) and shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX17 Antibody (NB200-352)(3)

Publications using NB200-352

• Lorgeoux RP, Pan Q, Le Duff Y et al. DDX17 promotes the production of infectious HIV-1 particles through modulating viral RNA packaging and translation frameshift. Virology 2013-06-11 [PMID: 23769241] (WB, Human)
• Naji S, Ambrus G, Cimerman?i? P et al. Host cell interactome of HIV-1 Rev includes RNA helicases involved in multiple facets of virus production. Mol Cell Proteomics 2012-04-01 [PMID: 22174317]
• Jin W, Chen Y, Di G-h et al. Estrogen Receptor (ER) {beta} or p53 Attenuates ER{alpha}-mediated Transcriptional Activation on the BRCA2 Promoter. J Biol Chem;283(44):29671-29680. 2008-01-01 [PMID: 18765668]

Bioinformatics

• Gene Symbol: DDX17
• Entrez:
  • 10521(Human)
• Uniprot:
  • Q92841()