DDX17 Antibody

Images

 
Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for ...read more
Immunohistochemistry: DDX17 Antibody [NB200-352] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate (50 ug) from NIH3T3, TCMK1,4T1,CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody
Biological Strategies: Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), MCF7 (M; 50 ug) and K562 (K; 50 ug) cells. Antibody used at 0.1 ug/ml.
Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352] - FFPE section of human seminoma. Affinity purified rabbit anti-DDX17 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

DDX17 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 550 and the C-terminus (residue 613) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 using the numbering given in entry AAC50787.1 (GeneID 10521).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX17
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:15000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
DDX17 Lysate (NBL1-09782)
DDX17 Lysate (NBP2-65905)
Publications
Read Publications using
NB200-352 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX17 Antibody

  • DDX17
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
  • DEAD box protein 17
  • DEAD box protein p72
  • DEAD/H box 17
  • DKFZp761H2016
  • EC 3.6.1
  • EC 3.6.4.13
  • p72
  • P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
  • probable ATP-dependent RNA helicase DDX17
  • RH70
  • RNA-dependent helicase p72

Background

DDX17 (p72) is a highly related member of the DEAD box family and is an established RNA helicase. It has been implicated in growth regulation and has been shown to be involved in both pre-mRNA and pre-rRNA processing. This protein has also been reported to act as a transcriptional co-activator for estrogen-receptor alpha (ERa) and shown to interact with co-activators p300/CBP and the RNA polymerase II holoenzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX17 Antibody (NB200-352)(3)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDX17
Entrez
Uniprot