DDX6 Antibody

Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. This image was submitted via customer Review.

Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.

Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).

• Reactivity: Hu, Mu, Ha, Ch, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX6 Antibody Summary

• Immunogen: The immunogen recognized by NB200-191 maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
• Predicted Species: Primate (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: ICC/IF was reported in scientific literature (PMID: 25800057) and a verified customer review. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Control:

  DDX6 Overexpression Lysate

Reactivity Notes

Hamster reactivity reported from a verified customer reivew. Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX6 Antibody

• ATP-dependent RNA helicase p54
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
• DEAD box protein 6
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
• EC 3.6.1
• EC 3.6.4.13
• FLJ36338
• HLR2DEAD box-6
• Oncogene RCK
• probable ATP-dependent RNA helicase DDX6
• RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX6 Antibody (NB200-191)(35)

Publications using NB200-191

• Sharma NR, Majerciak V, Kruhlak MJ et al. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182 Nucleic Acids Res. Aug 10 2019 [PMID: 31400113] (WB, Human)
• Paul S, Dansithong W, Figueroa KP et al. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun Sep 7 2018 [PMID: 30194296] (WB, Mouse)
• Hubstenberger A, Courel M, Benard M, Souquere S et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons Mol. Cell 2017 Oct 05 [PMID: 28965817] (ICC/IF, Human)
• Ayache Jessica, Benard Marianne, Ernoult-Lange Michele et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell 2015 [PMID: 25995375] (WB, Human)
• Somasekharan SP, El-Naggar A, Leprivier G et al. YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1. J. Cell Biol. 2015 Mar 30 [PMID: 25800057] (ICC/IF, Human)
• Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by promoting proteome remodeling on the mRNA cap bioRxiv Jul 14 2020 (ICC/IF, WB, Human)
• Cougnoux A, Drummond RA, Fellmeth M, et al. GC content shapes mRNA storage and decay in human cells Elife Dec 19 2019 [PMID: 31855182] (WB, ICC/IF, Human)
• de Vries S, Naarmann-de Vries IS, Urlaub H et al. Identification of DDX6 as a cellular modulator of VEGF expression under hypoxia. J Biol Chem 2013 Jan 4 [PMID: 23293030] (WB, ICC/IF, Human)
• Nihei Y, Ito D, Suzuki N. Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding Protein 43 (TDP-43) and Fused in Sarcoma (FUS) J Biol Chem 2012 Oct 9 [PMID: 23048034] (ICC/IF, Human)
• Novotny I, Podolska K, Blazikova M et al. Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins. Mol Biol Cell 2012 Oct [PMID: 22875987]
• Ernoult-Lange M, Baconnais S, Harper M et al. Multiple binding of repressed mRNAs by the P-body protein Rck/p54. RNA 2012 Sep [PMID: 22836354]
• Crist CG, Montarras D, Buckingham M et al. Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules. Cell Stem Cell 2012 Jul [PMID: 22770245]
• Cougot N, Cavalier A, Thomas D et al. The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography. J Mol Biol 2012 Jun [PMID: 22484175]
• Marnef A, Weil D, Standart N et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 2012 Jan [PMID: 22090346]
• Saito K, Kondo E, Matsushita M et al. MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6. Nucleic Acids Res 2011 Aug [PMID: 21486751]
• Totaro A, Renzi F, La Fata G et al. The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique. Nucleic Acids Res 2011 Jan [PMID: 20852261]
• Marnef A, Maldonado M, Bugaut A et al. Distinct functions of maternal and somatic Pat1 protein paralogs. RNA 2010 Nov [PMID: 20826699]
• Jangra RK, Yi M, Lemon SM et al. DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation. J Virol 2010 Jul [PMID: 20392846]
• Kemler I, Meehan A, Poeschla EM et al. Live-cell coimaging of the genomic RNAs and Gag proteins of two lentiviruses. J Virol 2010 Jul [PMID: 20392841]
• Savas JN, Ma B, Deinhardt K et al. A role for huntington disease protein in dendritic RNA granules. J Biol Chem 2010 Apr [PMID: 20185826]
• Emara MM, Ivanov P, Hickman T et al. Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. J Biol Chem 2010 Apr [PMID: 20129916]
• Holzel M, Orban M, Hochstatter J et al. Defects in 18 S or 28 S rRNA processing activate the p53 pathway. J Biol Chem 2010 Feb 26 [PMID: 20056613] (Human)
• Souquere S, Mollet S, Kress M et al. Unravelling the ultrastructure of stress granules and associated P-bodies in human cells. J Cell Sci 2009 Oct [PMID: 19812307]
• Taddeo et al. PNAS 106 (29):12139-12144. 2009 [PMID: 19584246]
• Otsuka et al. Mol.Cell.Biol. 29 (8):2155-2167. 2009 [PMID: 19223470]
• Cougot et al. J.Neurosci. 28 (51):13793-13804. 2008 [PMID: 19091970]
• Zhai et al. Mol.Cell.Biol. 28 (24):7414-7426. 2008 [PMID: 18936174]
• Ernoult-Lange et al. Mol.Biol.Cell 20 (1):176-187. 2009 [PMID: 18923137]
• Zheng et al. J.Cell Biol. 182 (1):89-101. 2008 [PMID: 18625844]
• Morris et al. Mol.Cell.Biol. 28 (12):4093-4103. 2008 [PMID: 18411299]
• Marin et al. J.Virol. 82 (2):987-998. 2008 [PMID: 17977970]
• Song Kiledjian. Song and Kiledjian. RNA 13 (12):2356-2365. 2007 [PMID: 17942740]
• Nonhoff, U, Ralser, M, Welzel, F, Piccini, I, Balzereit, D, Yaspo, M-L, Lehrach, H, Krobitsch, S. Ataxin-2 Interacts with the DEAD/H-Box RNA Helicase DDX6 Interferes with P-Bodies Stress Granules,. E06-12-1120. 2007 [PMID: 17392519]
• Chu CY, Rana TM. Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54. PLoS Biol 2006 Jul [PMID: 16756390]
• Wichroski MJ, Robb GB, Rana TM et al. Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies. PLoS Pathog 2006 May [PMID: 16699599]

Review for DDX6 Antibody (NB200-191) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human, Mouse, Hamster.

reviewed by: Lifeng Liu Human, Mouse, Hamster 08/22/2017

Summary

• Sample Tested: MEFs,BHK (Baby Hamster Kidney fibroblasts),U2OS cells,HOS human osteosarcoma cell line
• Species: Human, Mouse, Hamster
• Lot: A1

Comments

• Comments: Cells were seeded on coverslips in 12-well plates overnight. Then, cells were fixed with 3.7% formaldehyde in PBS and blocked and permeabilized in PBS containing 5% horse serum (Sigma) and 0.3% Triton X-100 for 16 h at 4° C, followed by incubation with primary antibodies: rabbit-anti-DDX6 (1:1000) (NB200-191) for 1 h at room temperature. Coverslips were then washed with PBS and incubated with secondary antibodies (Alexa 488-conjugated donkey-anti-rabbit (1:200) and Hoechst 33258, followed by one more washing step and mounting on glass slides.

Bioinformatics

• Gene Symbol: DDX6
• Entrez:
  • 1656(Human)
• Uniprot:
  • P26196(Human)