DDX6 Antibody

Images

 
Independent Antibodies: Western Blot: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity ...read more
Immunohistochemistry: DDX6 Antibody [NB200-191] - Sample: FFPE section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 ...read more
Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. This image ...read more

Product Details

Summary
Reactivity Hu, Mu, HaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

DDX6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Immunofluorescence 1:1000
Application Notes
Immunocytochemistry/Immunofluorescence was reported in scientific literature. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.
Positive Control
DDX6 Lysate (NBL1-09810)
Reviewed Applications
Read 1 Review rated 4
using
NB200-191 in the following applications:

Publications
Read Publications using
NB200-191 in the following applications:

Reactivity Notes

Hamster reactivity per customer reivew.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX6 Antibody

  • ATP-dependent RNA helicase p54
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DEAD box protein 6
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ36338
  • HLR2DEAD box-6
  • Oncogene RCK
  • probable ATP-dependent RNA helicase DDX6
  • RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-191
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IHC, IHC-P, IP, ICC/IF

Publications for DDX6 Antibody (NB200-191)(29)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(3)
WB
(1)
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(4)
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Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB200-191 Applications Species
Somasekharan SP, El-Naggar A, Leprivier G et al. YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1. J. Cell Biol. 2015 Mar 30 [PMID: 25800057] (ICC/IF, Human) ICC/IF Human
de Vries S, Naarmann-de Vries IS, Urlaub H et al. Identification of DDX6 as a cellular modulator of VEGF expression under hypoxia. J Biol Chem 2013 Jan 4 [PMID: 23293030] (WB, ICC/IF, Human) WB, ICC/IF Human
Nihei Y, Ito D, Suzuki N. Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding Protein 43 (TDP-43) and Fused in Sarcoma (FUS) J Biol Chem 2012 Oct 9 [PMID: 23048034] (ICC/IF, Human) ICC/IF Human
Novotny I, Podolska K, Blazikova M et al. Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins. Mol Biol Cell 2012 Oct [PMID: 22875987]
Ernoult-Lange M, Baconnais S, Harper M et al. Multiple binding of repressed mRNAs by the P-body protein Rck/p54. RNA 2012 Sep [PMID: 22836354]
Crist CG, Montarras D, Buckingham M et al. Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules. Cell Stem Cell 2012 Jul [PMID: 22770245]
Cougot N, Cavalier A, Thomas D et al. The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography. J Mol Biol 2012 Jun [PMID: 22484175]
Marnef A, Weil D, Standart N et al. RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor. Mol Biol Cell 2012 Jan [PMID: 22090346]
Saito K, Kondo E, Matsushita M et al. MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6. Nucleic Acids Res 2011 Aug [PMID: 21486751]
Totaro A, Renzi F, La Fata G et al. The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique. Nucleic Acids Res 2011 Jan [PMID: 20852261]
Show All 29 Publications.

Review for DDX6 Antibody (NB200-191) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human, Mouse, Hamster.

Reviews using NB200-191:
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Human, Mouse, Hamster
(1)
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Images Ratings Applications Species Date Details
 DDX6 NB200-191
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reviewed by:
Lifeng Liu
Human, Mouse, Hamster 08/22/2017
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Summary

Sample TestedMEFs,BHK (Baby Hamster Kidney fibroblasts),U2OS cells,HOS human osteosarcoma cell line
SpeciesHuman, Mouse, Hamster
LotA1

Comments

CommentsCells were seeded on coverslips in 12-well plates overnight. Then, cells were fixed with 3.7% formaldehyde in PBS and blocked and permeabilized in PBS containing 5% horse serum (Sigma) and 0.3% Triton X-100 for 16 h at 4° C, followed by incubation with primary antibodies: rabbit-anti-DDX6 (1:1000) (NB200-191) for 1 h at room temperature. Coverslips were then washed with PBS and incubated with secondary antibodies (Alexa 488-conjugated donkey-anti-rabbit (1:200) and Hoechst 33258, followed by one more washing step and mounting on glass slides.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DDX6 Antibody (NB200-191) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

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Diseases for DDX6 Antibody (NB200-191)

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Recent Reviews

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Lifeng Liu
08/22/2017
Application:
Species: Human, Mouse, Hamster

Bioinformatics

Gene Symbol DDX6
Entrez
Uniprot