DDX6 Antibody

Images

 
Western Blot: DDX6 Antibody [NB200-191] - Western blot analysis of LBCs show increased Staufen (STAU1) (NBP1-33202) levels compared with normal controls. DDX6 (NB200-191) levels are unchanged. HD and SCA3 patient (polyQ ...read more
Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted ...read more
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 ...read more
Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 ...read more
Orally administered lemongrass and white tea extract reduce tumor size in lymphoma xenograft model in immunocompromised miceImmunocompromised mice were subcutaneously injected with cancerous cells and tumors were ...read more

Product Details

Summary
Reactivity Hu, Mu, Ha, Ch, Pm-OrSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DDX6 Antibody Summary

Immunogen
The immunogen recognized by NB200-191 maps to a region between residues 1 and 50 of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
Predicted Species
Orangutan (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:5000-1:15000
Application Notes
ICC/IF was reported in scientific literature (PMID: 25800057) and a verified customer review. Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Control
DDX6 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB200-191 in the following applications:

Publications
Read Publications using
NB200-191 in the following applications:

Reactivity Notes

Hamster reactivity reported from a verified customer reivew.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS, 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX6 Antibody

  • ATP-dependent RNA helicase p54
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DEAD box protein 6
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ36338
  • HLR2DEAD box-6
  • Oncogene RCK
  • probable ATP-dependent RNA helicase DDX6
  • RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-191
Species: Hu, Mu, Ha, Ch, Pm-Or
Applications: WB, ICC/IF, IHC, IP

Publications for DDX6 Antibody (NB200-191)(43)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(9)
IHC-P
(1)
WB
(7)
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Filter By Species
Human
(11)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using NB200-191 Applications Species
Heim A, Niedermeier ML, Stengel F, Mayer TU The translation regulator Zar1l controls timing of meiosis in Xenopus oocytes Development (Cambridge, England) 2022-11-01 [PMID: 36278895]
Venkataramanan S, Gadek M, Calviello L et al. DDX3X and DDX3Y are redundant in protein synthesis RNA 2021-12-01 [PMID: 34535544]
Paul S, Dansithong W, Figueroa KP et al. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun 2018-09-07 [PMID: 30194296] (WB, Mouse) WB Mouse
Mehravar M, Kumar Y, Olshansky M et al. MOV10 facilitates messenger RNA decay in an N6-methyladenosine (m6A) dependent manner to maintain the mouse embryonic stem cells state bioRxiv 2021-08-12
Sung CC, Poll B, Lin SH et al. Early Molecular Events Mediating Loss of Aquaporin-2 during Ureteral Obstruction in Rat Journal of the American Society of Nephrology : JASN 2022-08-02 [PMID: 35918145] (IHC-P, Rat)

Details:
Dilutions: 1:500
IHC-P Rat
Hallacli E, Kayatekin C, Nazeen S et al. The Parkinson's disease protein alpha-synuclein is a modulator of processing bodies and mRNA stability Cell 2022-06-09 [PMID: 35688132] (ICC/IF, WB, Human) ICC/IF, WB Human
Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by influencing protein-protein interactions on the mRNA cap Journal of cell science 2021-11-29 [PMID: 34841428]
Shah A, Bhandari R IP6K1 upregulates the formation of processing bodies by promoting proteome remodeling on the mRNA cap bioRxiv 2020-07-14 [PMID: 34841428] (WB, ICC/IF, Human) WB, ICC/IF Human
Somasekharan SP, Gleave M SARS-CoV-2 nucleocapsid protein interacts with immunoregulators and stress granules and phase separates to form liquid droplets FEBS letters 2021-11-15 [PMID: 34780058] (ICC/IF, Human) ICC/IF Human
Rink MR, Baptista MAP, Flomm FJ Et al. Concatemeric Broccoli reduces mRNA stability and induces aggregates PloS one 2021-08-04 [PMID: 34347781] (ICC/IF) ICC/IF
Show All 43 Publications.

Review for DDX6 Antibody (NB200-191) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Hamster, Human, Mouse.

Reviews using NB200-191:
Filter by Applications
Immunofluorescence and microscopy
(1)
All Applications
Filter by Species
Hamster, Human, Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Other DDX6 NB200-191
Enlarge
4
reviewed by:
Lifeng Liu
Immunofluorescence and microscopy Hamster, Human, Mouse 08/22/2017
View

Summary

ApplicationOther
Sample TestedBHK (Baby Hamster Kidney fibroblasts),HOS human osteosarcoma cell line,MEFs,U2OS cells
SpeciesHamster, Human, Mouse
LotA1

Comments

CommentsCells were seeded on coverslips in 12-well plates overnight. Then, cells were fixed with 3.7% formaldehyde in PBS and blocked and permeabilized in PBS containing 5% horse serum (Sigma) and 0.3% Triton X-100 for 16 h at 4° C, followed by incubation with primary antibodies: rabbit-anti-DDX6 (1:1000) (NB200-191) for 1 h at room temperature. Coverslips were then washed with PBS and incubated with secondary antibodies (Alexa 488-conjugated donkey-anti-rabbit (1:200) and Hoechst 33258, followed by one more washing step and mounting on glass slides.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DDX6 Antibody (NB200-191) (0)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Lifeng Liu
08/22/2017
Application: Immunofluorescence and microscopy
Species: Hamster, Human, Mouse

Bioinformatics

Gene Symbol DDX6
Entrez
Uniprot