DDX6 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted ...read more
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 ...read more
Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 ...read more

Product Details

Summary
Reactivity Hu, Mu, Ha, Ch, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DDX6 Antibody Summary

Immunogen
The epitope recognized by NB200-191 maps to a region between residue 1050 and the Cterminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Predicted Species
Primate (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
ICC/IF was reported in scientific literature (PMID: 25800057). Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Control
DDX6 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB200-191 in the following applications:

Publications
Read Publications using
NB200-191 in the following applications:

  • 4 publications
  • WB
    4 publications

Reactivity Notes

Hamster reactivity per customer reivew. Orangutan (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX6 Antibody

  • ATP-dependent RNA helicase p54
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DEAD box protein 6
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ36338
  • HLR2DEAD box-6
  • Oncogene RCK
  • probable ATP-dependent RNA helicase DDX6
  • RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to the cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX6 Antibody (NB200-191)(33)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(4)
WB
(4)
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Human
(7)
Mouse
(1)
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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB200-191 Applications Species
Sharma NR, Majerciak V, Kruhlak MJ et al. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182 Nucleic Acids Res. Aug 10 2019 [PMID: 31400113] (WB, Human) WB Human
Paul S, Dansithong W, Figueroa KP et al. Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration. Nat Commun Sep 7 2018 [PMID: 30194296] (WB, Mouse) WB Mouse
Hubstenberger A, Courel M, Benard M, Souquere S et al. P-Body Purification Reveals the Condensation of Repressed mRNA Regulons Mol. Cell 2017 Oct 05 [PMID: 28965817] (ICC/IF, Human) ICC/IF Human
Ayache Jessica, Benard Marianne, Ernoult-Lange Michele et al. P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes. Mol Biol Cell 2015 [PMID: 25995375] (WB, Human) WB Human
Somasekharan SP, El-Naggar A, Leprivier G et al. YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1. J. Cell Biol. 2015 Mar 30 [PMID: 25800057] (ICC/IF, Human) ICC/IF Human
de Vries S, Naarmann-de Vries IS, Urlaub H et al. Identification of DDX6 as a cellular modulator of VEGF expression under hypoxia. J Biol Chem 2013 Jan 4 [PMID: 23293030] (WB, ICC/IF, Human) WB, ICC/IF Human
Nihei Y, Ito D, Suzuki N. Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding Protein 43 (TDP-43) and Fused in Sarcoma (FUS) J Biol Chem 2012 Oct 9 [PMID: 23048034] (ICC/IF, Human) ICC/IF Human
Novotny I, Podolska K, Blazikova M et al. Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins. Mol Biol Cell 2012 Oct [PMID: 22875987]
Ernoult-Lange M, Baconnais S, Harper M et al. Multiple binding of repressed mRNAs by the P-body protein Rck/p54. RNA 2012 Sep [PMID: 22836354]
Crist CG, Montarras D, Buckingham M et al. Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules. Cell Stem Cell 2012 Jul [PMID: 22770245]
Show All 33 Publications.

Review for DDX6 Antibody (NB200-191) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human, Mouse, Hamster.

Reviews using NB200-191:
Filter by Applications
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Human, Mouse, Hamster
(1)
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Images Ratings Applications Species Date Details
 DDX6 NB200-191
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reviewed by:
Lifeng Liu
Human, Mouse, Hamster 08/22/2017
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Summary

Sample TestedMEFs,BHK (Baby Hamster Kidney fibroblasts),U2OS cells,HOS human osteosarcoma cell line
SpeciesHuman, Mouse, Hamster
LotA1

Comments

CommentsCells were seeded on coverslips in 12-well plates overnight. Then, cells were fixed with 3.7% formaldehyde in PBS and blocked and permeabilized in PBS containing 5% horse serum (Sigma) and 0.3% Triton X-100 for 16 h at 4° C, followed by incubation with primary antibodies: rabbit-anti-DDX6 (1:1000) (NB200-191) for 1 h at room temperature. Coverslips were then washed with PBS and incubated with secondary antibodies (Alexa 488-conjugated donkey-anti-rabbit (1:200) and Hoechst 33258, followed by one more washing step and mounting on glass slides.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DDX6 Antibody (NB200-191) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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4
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2
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1
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Lifeng Liu
08/22/2017
Application:
Species: Human, Mouse, Hamster

Bioinformatics

Gene Symbol DDX6
Entrez
Uniprot