DDX5 Antibody

Images

 
Biological Strategies: Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN ...read more
Immunohistochemistry: DDX5 Antibody [NB200-351] - ~~ Sample: FFPE section of mouse renal cell carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. ...read more
Independent Antibodies: Western Blot: DDX5 Antibody [NB200-351] - Detection of DDX5 in whole cell lysates from HeLa (5, 15, 50 ug), 293 (T; 50 ug), and MCF7 (M; 50 ug), and K562 (K; 50 ug) for WB (A) and HeLa (1 ...read more
Immunohistochemistry: DDX5 Antibody [NB200-351] - ~ ~Sample: FFPE section of human breast carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

DDX5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 614) of human DEAD (Asp-Glu-Ala-Asp) Polypeptide 5 using the numbering given in entry NP_004387.1 (GeneID 1655).
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 mg/mg
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Immunoprecipitation: the antibody may be diluted further than indicated. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DDX5 Lysate (NBL1-09801)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB200-351 in the following applications:

Publications
Read Publications using
NB200-351 in the following applications:

  • WB
    2 publications

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan and Chimpanzee

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX5 Antibody

  • B-cell CLL/lymphoma 7 protein family member C
  • B-cell CLL/lymphoma 7C
  • DDX5
  • DEAD/H
  • G17P1
  • HELR
  • HLR1
  • HUMP68

Background

DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX5 Antibody (NB200-351)(5)

Review for DDX5 Antibody (NB200-351) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: the baby hamster kidney cell line (BHK-21).

Reviews using NB200-351:
Filter by Applications
WB
(1)
All Applications
Filter by Species
the baby hamster kidney cell line (BHK-21)
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot DDX5 NB200-351
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reviewed by:
Anonymous
WB the baby hamster kidney cell line (BHK-21) 12/13/2017
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Summary

ApplicationWestern Blot
Sample Testedthe baby hamster kidney cell line (BHK-21)
Speciesthe baby hamster kidney cell line (BHK-21)
LotNB200-351

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for DDX5 Antibody (NB200-351). (Showing 1 - 1 of 1 FAQs).

  1. How long is the DDX5 antibody NB200-351 stable at 4C?
    • We guarantee our antibodies for 6 months after the date of receipt given that the product has been stored correctly. NB200-351 contains sodium azide, which is a very effective stabilizer and even though this antibody is past our guarantee coverage date, I would expect it to perform well.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for DDX5 Antibody (NB200-351)

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Diseases for DDX5 Antibody (NB200-351)

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Pathways for DDX5 Antibody (NB200-351)

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Blogs on DDX5

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Recent Reviews

5
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1
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2
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1
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Anonymous
12/13/2017
Application: WB
Species: the baby hamster kidney cell line (BHK-21)

Bioinformatics

Gene Symbol DDX5
Entrez
Uniprot