DDX5 Antibody

Western Blot: DDX5 Antibody [NB200-351] - Detection of Human and Mouse DDX5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody NB200-351 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of human DDX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

Western Blot: DDX5 Antibody [NB200-351] - Detection of DDX5 in whole cell lysates from HeLa (5, 15, 50 ug), 293 (T; 50 ug), and MCF7 (M; 50 ug), and K562 (K; 50 ug) for WB (A) and HeLa (1 mg) for IP (B). Antibodies: NB 200-351 used at 0.04 ug/ml for WB (A & B) and at 3 ug/ml lysate for IP (B). DDX5 was also immonuprecipitated with NB 200-350 used at 3 ug/ml lysate. Detection: ECL 30 seconds.

Immunohistochemistry: DDX5 Antibody [NB200-351] - ~ ~Sample: FFPE section of human breast carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunohistochemistry: DDX5 Antibody [NB200-351] - ~~ Sample: FFPE section of mouse renal cell carcinoma.~ Antibody: Affinity purified rabbit anti-DDX5 ~Cat. No. Lot2 used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Immunohistochemistry: DDX5 Antibody [NB200-351] - Detection of mouse DDX5 by immunohistochemistry. Sample: FFPE section of mouse skin. Antibody: Affinity purified rabbit anti-DDX5 NB200-351 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: DDX5 Antibody [NB200-351] - Detection of human DDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX5 antibody NB200-351 (lot NB200-351-2) used for IP at 6 ug per reaction. DDX5 was also immunoprecipitated by a previous lot of this antibody (lot NB200-351-1) and another rabbit anti-DDX5 antibody. For blotting immunoprecipitated DDX5, NB200-351 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Mu, Ha, Pm
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX5 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 614) of human DEAD (Asp-Glu-Ala-Asp) Polypeptide 5 using the numbering given in entry NP_004387.1 (GeneID 1655).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2500-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Immunoprecipitation: the antibody may be diluted further than indicated. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  DDX5 Overexpression Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 28516729). Based on 100% sequence identity, this antibody is predicted to react with Orangutan and Chimpanzee

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX5 Antibody

• B-cell CLL/lymphoma 7 protein family member C
• B-cell CLL/lymphoma 7C
• DDX5
• DEAD/H
• G17P1
• HELR
• HLR1
• HUMP68

Background

DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX5 Antibody (NB200-351)(5)

Publications using NB200-351

• Sun X, Wang S, Lin X et al. Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells. Proteomics 2017-06-20 [PMID: 28516729] (WB, Hamster)
• Muller RY, Hammond MC, Rio DC, Lee YJ. An Efficient Method for Electroporation of Small Interfering RNAs into ENCODE Project Tier 1 GM12878 and K562 Cell Lines. J Biomol Tech. 2015-10-29 [PMID: 26543439] (WB, Human)
• Saporita AJ, Chang HC, Winkeler CL et al. RNA helicase DDX5 is a p53-independent target of ARF that participates in ribosome biogenesis. Cancer Res 2011-11-01 [PMID: 21937682]
• Guo J, Hong F, Loke J et al. A DDX5 S480A polymorphism is associated with increased transcription of fibrogenic genes in hepatic stellate cells. J Biol Chem 2010-02-01 [PMID: 20022962]
• Chiu YL, Witkowska HE, Hall SC et al. High-molecular-mass APOBEC3G complexes restrict Alu retrotransposition. Proc Natl Acad Sci U S A 2006-10-01 [PMID: 17030807]

Review for DDX5 Antibody (NB200-351) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: the baby hamster kidney cell line (BHK-21).

reviewed by: Xin Sun WB the baby hamster kidney cell line (BHK-21) 12/13/2017

Summary

• Application: Western Blot
• Sample Tested: the baby hamster kidney cell line (BHK-21)
• Species: the baby hamster kidney cell line (BHK-21)
• Lot: NB200-351

FAQs for DDX5 Antibody (NB200-351). (Showing 1 - 1 of 1 FAQs).

1. How long is the DDX5 antibody NB200-351 stable at 4C?
   • We guarantee our antibodies for 6 months after the date of receipt given that the product has been stored correctly. NB200-351 contains sodium azide, which is a very effective stabilizer and even though this antibody is past our guarantee coverage date, I would expect it to perform well.

Bioinformatics

• Gene Symbol: DDX5
• Entrez:
  • 1655(Human)
• Uniprot:
  • P17844(Human)