DDX17 Antibodies
We offer DDX17 Antibodies for use in common research applications: Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each DDX17 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our DDX17 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the DDX17 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our DDX17 polyclonal antibodies.
Alternate Names for DDX17 Antibodies
anti-DDX17 antibody, anti-DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 antibody, anti-DEAD box protein 17 antibody, anti-DEAD box protein p72 antibody, anti-DEAD/H box 17 antibody, anti-DKFZp761H2016 antibody, anti-EC 3.6.1 antibody, anti-EC 3.6.4.13 antibody, anti-P72DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD) antibody, anti-probable ATP-dependent RNA helicase DDX17 antibody, anti-RH70 antibody, anti-RNA-dependent helicase p72 antibody
8 Results for "ddx17" in Primary Antibodies
![Western Blot: DDX17 Antibody (2U1P0) [NBP3-16607] - Western blot analysis of extracts of various cell lines, using DDX17 Rabbit mAb (NBP3-16607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/DDX17-Antibody-2U1P0-Western-Blot-NBP3-16607-img0004.jpg "Western Blot: DDX17 Antibody (2U1P0) [NBP3-16607] - Western blot analysis of extracts of various cell lines, using DDX17 Rabbit mAb (NBP3-16607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: DDX17 Antibody (2U1P0) [NBP3-16607] - Western blot analysis of extracts of various cell lines, using DDX17 Rabbit mAb (NBP3-16607) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/DDX17-Antibody-2U1P0-Western-Blot-NBP3-16607-img0005.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded rat spleen tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-7220251245349.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded mouse intestin tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-72202512443388.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded mouse testis tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-72202512435383.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded rat kidney tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-722025124533.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded human cervix cancer tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-72202512443310.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded mouse brain tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-722025124536.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded mouse lung tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-7220251245397.jpg)
![Immunohistochemistry: DDX17 Antibody (2U1P0) [NBP3-16607] - Immunohistochemistry analysis of DDX17 in paraffin-embedded rat brain tissue using DDX17 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16607_rabbit-ddx17-mab-2u1p0-72202512435394.jpg)
![Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NB200-352-img0009.jpg "Western Blot: DDX17 Antibody [NB200-352] - Detection of Human DDX17 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody NB200-352 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![Immunohistochemistry-Paraffin: DDX17 Antibody [NB200-352] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- DDX17 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunohistochemistry-Paraffin-NB200-352-img0008.jpg)
![Western Blot: DDX17 Antibody [NB200-352] - Whole cell lysate (50 ug) from NIH3T3, TCMK1,4T1,CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-DDX17 antibody](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NB200-352-img0005.jpg)
![Western Blot: DDX17 Antibody (JE35-01) [NBP3-32261] - Western blot analysis of DDX17 on different lysates with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-OV-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 80 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32261_rabbit-ddx17-mab-je35-01-27820241631647.jpg "Western Blot: DDX17 Antibody (JE35-01) [NBP3-32261] - Western blot analysis of DDX17 on different lysates with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-OV-3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 80 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32261) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: DDX17 Antibody (JE35-01) [NBP3-32261] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32261) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32261_rabbit-ddx17-mab-je35-01-29820241492631.jpg)
![Immunocytochemistry/ Immunofluorescence: DDX17 Antibody (JE35-01) [NBP3-32261] - Immunocytochemistry analysis of MCF7 cells labeling DDX17 with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDX17 antibody (NBP3-32261) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32261_rabbit-ddx17-mab-je35-01-2920241192387.jpg)
![Flow Cytometry: DDX17 Antibody (JE35-01) [NBP3-32261] - Flow cytometric analysis of MCF7 cells labeling DDX17. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32261, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32261_rabbit-ddx17-mab-je35-01-29202416372191.jpg)
![Immunohistochemistry-Paraffin: DDX17 Antibody [NBP2-38985] - Staining in human epididymis and skeletal muscle tissues using anti-DDX17 antibody. Corresponding DDX17 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunohistochemistry-Paraffin-NBP2-38985-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DDX17 Antibody [NBP2-38985] - Staining in human epididymis and skeletal muscle tissues using anti-DDX17 antibody. Corresponding DDX17 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: DDX17 Antibody [NBP2-38985] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NBP2-38985-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DDX17 Antibody [NBP2-38985] - Immunofluorescent staining of human cell line HEK 293 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38985-img0004.jpg)
![Immunohistochemistry-Paraffin: DDX17 Antibody [NBP2-38985] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunohistochemistry-Paraffin-NBP2-38985-img0006.jpg)
![Immunohistochemistry-Paraffin: DDX17 Antibody [NBP2-38985] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunohistochemistry-Paraffin-NBP2-38985-img0007.jpg)
![Western Blot: DDX17 Antibody [NBP2-87262] - WB Suggested Anti-DDX17 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysateDDX17 is supported by BioGPS gene expression data to be expressed in Jurkat](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NBP2-87262-img0003.jpg "Western Blot: DDX17 Antibody [NBP2-87262] - WB Suggested Anti-DDX17 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysateDDX17 is supported by BioGPS gene expression data to be expressed in Jurkat")
![Immunohistochemistry-Paraffin: DDX17 Antibody [NBP2-87262] - Paraffin Embedded Tissue: Human alveolar cell. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunohistochemistry-Paraffin-NBP2-87262-img0002.jpg)
![Immunohistochemistry-Paraffin: DDX17 Antibody [NBP2-87262] - Paraffin Embedded Tissue: Human Kidney. Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X](http://images.novusbio.com/fullsize/DDX17-Antibody-Immunohistochemistry-Paraffin-NBP2-87262-img0001.jpg)
![Western Blot: DDX17 Antibody (SR2137) [NBP3-22156] - Western blot analysis using NBP3-22156 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-22156_rabbit-ddx17-mab-sr2137-57202317161883.jpg "Western Blot: DDX17 Antibody (SR2137) [NBP3-22156] - Western blot analysis using NBP3-22156 in 293T cell lysate.")
![Western Blot: DDX17 Antibody [NBP2-87261] - WB Suggested Anti-DDX17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateDDX17 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells](http://images.novusbio.com/fullsize/DDX17-Antibody-Western-Blot-NBP2-87261-img0001.jpg "Western Blot: DDX17 Antibody [NBP2-87261] - WB Suggested Anti-DDX17 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysateDDX17 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells")