IFIT2 Antibody

Images

 
Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP1-31164] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term diluted at 1:200. Red: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

IFIT2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IFIT2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFIT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-31164 in the following applications:

Publications
Read Publications using
NBP1-31164 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IFIT2 Antibody

  • cig42
  • G10P2IFI-54K
  • GARG-39
  • IFI-54
  • IFI54IFIT-2
  • IFI-54K
  • Interferon, alpha-inducible protein (MW 54kD)
  • Interferon-induced 54 kDa protein
  • interferon-induced protein 54
  • interferon-induced protein with tetratricopeptide repeats 2
  • ISG-54 K
  • ISG54
  • ISG-54K
  • P54

Background

Saha et al. used this antibody to study protein expression of murine GARG39/IFIT2 in relation to viral infection. Immunofluorescence localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western blotting shows approx. 55 kD band GARG39/IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine GARG39/IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), interferon-induced 54 kDa protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% amino acid sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), Interferon-induced 54 kDa protein (IFI-54K) and ISG-54 K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFIT2 Antibody (NBP1-31164)(3)

Reviews for IFIT2 Antibody (NBP1-31164) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP1-31164:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 07/17/2015
View

Summary

ApplicationWestern Blot
Sample Tested293T, Hela, HEC-1-B, MRC-5 fibroblasts, whole cell extract following Senadi virus infection for 8-24h (200HAU/ml)
SpeciesHuman

Blocking

Blocking Details5% skimmed milk in TBS-T at room temp

Primary Anitbody

Dilution Ratio1/1000, 2 or 3h at room temp, 5% milk in TBS-T

Secondary Antibody

Secondary DescriptionAnti-Rabbit IgG, HRP
Secondary Manufacturer Cat#KPL (Cat# 074-1506)
Secondary Concentration1/10000

Details

Detection NotesWestern Lightning ECL kit (Perkin-Elmer, Waltham, MA), exposure time 30sec-1min
 
reviewed by:
Anonymous
WB Human 07/16/2015
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman

Blocking

Blocking Details5% skimmed milk in TBS-T at room temp

Primary Anitbody

Dilution Ratio1/1000, 2 or 3h at room temp, 5% milk in TBS-T

Secondary Antibody

Secondary DescriptionAnti-Rabbit IgG, HRP
Secondary Manufacturer Cat#KPL (Cat# 074-1506)
Secondary Concentration1/10000

Details

Detection NotesWestern Lightning ECL kit (Perkin-Elmer, Waltham, MA), exposure time 30sec-1min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IFIT2 Antibody (NBP1-31164) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for IFIT2 Antibody (NBP1-31164)

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Blogs on IFIT2.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Anonymous
07/17/2015
Application: WB
Species: Human

 
Anonymous
07/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol IFIT2