Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding ...read more
Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP1-31164] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term diluted at 1:200. Red: ...read more
Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 2 Reviews rated 5 using NBP1-31164 in the following applications:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Alternate Names for IFIT2 Antibody
Interferon, alpha-inducible protein (MW 54kD)
Interferon-induced 54 kDa protein
interferon-induced protein 54
interferon-induced protein with tetratricopeptide repeats 2
Saha et al. used this antibody to study protein expression of murine GARG39/IFIT2 in relation to viral infection. Immunofluorescence localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western blotting shows approx. 55 kD band GARG39/IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine GARG39/IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), interferon-induced 54 kDa protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% amino acid sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), Interferon-induced 54 kDa protein (IFI-54K) and ISG-54 K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for IFIT2 Antibody (NBP1-31164)(3)
We have publications tested in 1 confirmed species: Human.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.