IFIT2 Antibody

Images

 
Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding ...read more
Immunocytochemistry/ Immunofluorescence: IFIT2 Antibody [NBP1-31164] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term diluted at 1:200. Red: ...read more
Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

IFIT2 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IFIT2. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IFIT2
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-31164 in the following applications:

  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for IFIT2 Antibody

  • cig42
  • G10P2IFI-54K
  • GARG-39
  • IFI-54
  • IFI54IFIT-2
  • IFI-54K
  • Interferon, alpha-inducible protein (MW 54kD)
  • Interferon-induced 54 kDa protein
  • interferon-induced protein 54
  • interferon-induced protein with tetratricopeptide repeats 2
  • ISG-54 K
  • ISG54
  • ISG-54K
  • P54

Background

Saha et al. used this antibody to study protein expression of murine GARG39/IFIT2 in relation to viral infection. Immunofluorescence localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western blotting shows approx. 55 kD band GARG39/IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine GARG39/IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), interferon-induced 54 kDa protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% amino acid sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), Interferon-induced 54 kDa protein (IFI-54K) and ISG-54 K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IFIT2 Antibody (NBP1-31164)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(4)
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Human
(4)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on IFIT2.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also ...  Read full blog post.

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Bioinformatics

Gene Symbol IFIT2