IFIT2 Antibody

Western Blot: IFIT2 Antibody [NBP1-31164] - p115 and Sec16A are required for optimal IRF-3 activation in response to activation of cytosolic RNA and DNA sensors.HeLa cells were infected with lentiviral vectors encoding shRNA targeting p115. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002747) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: IFIT2 Antibody [NBP1-31164] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IFIT2 protein stained by IFIT2 antibody [N1], N-term diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.

Western Blot: IFIT2 Antibody [NBP1-31164] - Sample(30 ug of whole cell lysate)A:A431 B:Hep G2 7.5% SDS PAGE, antibody diluted at 1:1000.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IFIT2 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IFIT2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IFIT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for IFIT2 Antibody

• cig42
• G10P2IFI-54K
• GARG-39
• IFI-54
• IFI54IFIT-2
• IFI-54K
• Interferon, alpha-inducible protein (MW 54kD)
• Interferon-induced 54 kDa protein
• interferon-induced protein 54
• interferon-induced protein with tetratricopeptide repeats 2
• ISG-54 K
• ISG54
• ISG-54K
• P54

Background

Saha et al. used this antibody to study protein expression of murine GARG39/IFIT2 in relation to viral infection. Immunofluorescence localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western blotting shows approx. 55 kD band GARG39/IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine GARG39/IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), interferon-induced 54 kDa protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% amino acid sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT-2), Interferon-induced 54 kDa protein (IFI-54K) and ISG-54 K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IFIT2 Antibody (NBP1-31164)(3)

Publications using NBP1-31164

• Khan KA, Do F, Marineau A et al. Fine-Tuning of the RIG-I-Like Receptor/Interferon Regulatory Factor 3-Dependent Antiviral Innate Immune Response by the Glycogen Synthase Kinase 3/beta-Catenin Pathway. Mol Cell Biol 2015 Sep 1 [PMID: 26100021]
• Baril M, Es-Saad S, Chatel-Chaix L et al. Genome-wide RNAi Screen Reveals a New Role of a WNT/CTNNB1 Signaling Pathway as Negative Regulator of Virus-induced Innate Immune Responses. PLoS Pathog 2013 Jun [PMID: 23785285] (WB, Human)
• van Zuylen WJ, Doyon P, Clement JF et al. Proteomic Profiling of the TRAF3 Interactome Network Reveals a New Role for the ER-to-Golgi Transport Compartments in Innate Immunity plos Pathog 2012 Jul [PMID: 22792062] (WB, Human)

Reviews for IFIT2 Antibody (NBP1-31164) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Anonymous WB Human 07/17/2015

Summary

• Application: Western Blot
• Sample Tested: 293T, Hela, HEC-1-B, MRC-5 fibroblasts, whole cell extract following Senadi virus infection for 8-24h (200HAU/ml)
• Species: Human

Blocking

• Blocking Details: 5% skimmed milk in TBS-T at room temp

Primary Anitbody

• Dilution Ratio: 1/1000, 2 or 3h at room temp, 5% milk in TBS-T

Secondary Antibody

• Secondary Description: Anti-Rabbit IgG, HRP
• Secondary Manufacturer Cat#: KPL (Cat# 074-1506)
• Secondary Concentration: 1/10000

Details

• Detection Notes: Western Lightning ECL kit (Perkin-Elmer, Waltham, MA), exposure time 30sec-1min

reviewed by: Anonymous WB Human 07/16/2015

Summary

• Application: Western Blot
• Sample Tested: 293T
• Species: Human

Blocking

• Blocking Details: 5% skimmed milk in TBS-T at room temp

Primary Anitbody

• Dilution Ratio: 1/1000, 2 or 3h at room temp, 5% milk in TBS-T

Secondary Antibody

• Secondary Description: Anti-Rabbit IgG, HRP
• Secondary Manufacturer Cat#: KPL (Cat# 074-1506)
• Secondary Concentration: 1/10000

Details

• Detection Notes: Western Lightning ECL kit (Perkin-Elmer, Waltham, MA), exposure time 30sec-1min

Bioinformatics

• Gene Symbol: IFIT2