CXCR5 Antibody (JB11-40)

Western Blot: CXCR5 Antibody (JB11-40) [NBP2-75460] - Western blot analysis of CXCR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Daudi cell lysate Lane 2: Raji cell lysate

Immunohistochemistry-Paraffin: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of paraffin-embedded rat kidney tissue using anti-CXCR5 antibody. Counter stained with hematoxylin.

Flow Cytometry: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of Daudi cells with CXCR5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of paraffin-embedded human tonsil tissue using anti-CXCR5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CXCR5 Antibody (JB11-40) [NBP2-75460] - Analysis of paraffin-embedded mouse heart tissue using anti-CXCR5 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: JB11-40
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CXCR5 Antibody (JB11-40) Summary

• Description: Novus Biologicals Rabbit CXCR5 Antibody (JB11-40) (NBP2-75460) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human CXCR5. (SwissProt: P32302 Human; SwissProt: Q04683 Mouse; SwissProt: P34997 Rat)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CXCR5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CXCR5 Antibody (JB11-40)

• BLR1
• BLR1MDR-15
• Burkitt lymphoma receptor 1
• Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif)receptor 5)
• Burkitt lymphoma receptor 1, GTP-binding protein
• CD185 antigen
• CD185
• chemokine (C-X-C motif) receptor 5
• C-X-C chemokine receptor type 5
• CXCR5
• CXC-R5
• MDR15CXCR-5
• MGC117347
• Monocyte-derived receptor 15

Background

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Chemokines activate leukocytes by binding to G protein coupled receptors (1). The ever-growing chemokine receptor subtypes can be divided into 2 major groups, CXCR and CCR, based on the 2 major classes of chemokines. One of the CCR receptors, CCR1, is expressed on neutrophils, monocytes, lymphocytes, and eosinophils and binds the leukocyte chemoattractant and hemopoiesis regulator macrophage-inflammatory protein (MIP-1 ), eotaxin, as well as several other related chemokines (2-4). Mice lacking the chemokine receptor CCR1 have defects in neutrophil trafficking and proliferation (5,6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCR5