CXCR3 Antibody

Western Blot: CXCR3 Antibody [NB100-56404] - HEK293T cells. Image from verified customer review.

Immunohistochemistry-Paraffin: CXCR3 Antibody [NB100-56404] - IHC analysis of a FFPE human spleen using 1:25 dilution of CXCR3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of HIER using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (40X) was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Western Blot: CXCR3 Antibody [NB100-56404] - Analysis of CXCR3 in human pancreas lysate using CXCR3 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CXCR3 Antibody Summary

• Immunogen: This antibody was developed against a synthetic peptide corresponding to amino acids 290-303 of human CXCR3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
• Application Notes: IHC in paraformaldehyde fixed frozen tissue is described in publication PMID: 19234157. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CXCR3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CXCR3 Antibody

• CD183 antigen
• CD183
• chemokine (C-X-C motif) receptor 3
• chemokine (C-X-C) receptor 3
• CKR-L2IP-10 receptor
• CMKAR3
• C-X-C chemokine receptor type 3
• CXCR3
• CXC-R3
• CXCR-3
• G protein-coupled receptor 9CD182
• GPR9
• GPR9Mig-R
• Interferon-inducible protein 10 receptor
• IP10 receptor
• IP10-R
• Mig receptor
• MigR

Background

Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Historically, CD183 was the third CXC chemokine receptor discovered and therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. The CXC chemokine receptor (CXCR) 3, a G-protein-coupled 7-transmembrane receptor, is expressed at high levels on activated and memory T cells, B cells, natural killer cells, and plasmacytoid monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR3 Antibody (NB100-56404)(2)

Publications using NB100-56404

• Suttorp CM, Cremers NA, van Rheden R et al. Chemokine Signaling during Midline Epithelial Seam Disintegration Facilitates Palatal Fusion Front Cell Dev Biol. 2017 Oct 30 [PMID: 29164113] (IHC-P, Mouse)
  
  Details:
  Used CXCR3 IHC antibody from Novus to quantify CXCR3 in palatal shelve mesenchyme
• Brambilla R, Persaud T, Hu X et al. Transgenic Inhibition of Astroglial NF-{kappa}B Improves Functional Outcome in Experimental Autoimmune Encephalomyelitis by Suppressing Chronic Central Nervous System Inflammation. J Immunol;182(5):2628-2640. 2009 [PMID: 19234157]

Review for CXCR3 Antibody (NB100-56404) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 09/11/2014

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human

Blocking

• Blocking Details: 5% BSA in PBS

Primary Anitbody

• Dilution Ratio: 1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

• Secondary Description: anti-rabbit IgG HRP conjugate (Jackson Labs)
• Secondary Manufacturer Cat#: 111-005-045
• Secondary Concentration: 1:20000

Details

• Detection Notes: Probed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

FAQs for CXCR3 Antibody (NB100-56404). (Showing 1 - 2 of 2 FAQs).

1. Regarding recommended dilutions the manual contains: "Immunohistochemistry-Paraffin, Western Blot 1-3 ug/ml" Does it mean 1-3 ug/ml is recommended for both applications or there is simply no recommended dilution IHC-P?
   • For our product NB100-56404, we did not have a dilution recommendation listed for IHC on our datasheet. I am sorry for any confusion. This application was added to our product based on a publication that actually uses this product at 1:200 in paraformaldehyde fixed frozen tissue samples: NCBI site.
2. Which isoform of CXCR3 is this antibody specific for?
   • NB100-56404 can only detect human CXCR3 isoforms 1 and 2 in WB and IHC-Fr, the validated applications.

Bioinformatics

• Gene Symbol: CXCR3
• Entrez:
  • 2833(Human)
  • 12766(Mouse)
• Uniprot:
  • P49682()