Western Blot: CXCR3 Antibody [NB100-56404] - 293T cells. Image from verified customer review.
Western Blot: CXCR3 Antibody [NB100-56404] - Analysis of CXCR3 in human pancreas lysate using CXCR3 antibody at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.05% BSA
0.05% Sodium Azide
Protein G purified
Western Blot 1-3 ug/ml
IHC in paraformaldehyde fixed frozen tissue is described in publication PMID: 19234157.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
41 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NB100-56404 in the following applications:
Chemokines play important roles in inflammation and critical for the recruitment of effector immune cells to sites of infection. Historically, CD183 was the third CXC chemokine receptor discovered and therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. The CXC chemokine receptor (CXCR) 3, a G-protein-coupled 7-transmembrane receptor, is expressed at high levels on activated and memory T cells, B cells, natural killer cells, and plasmacytoid monocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CXCR3 Antibody (NB100-56404). (Showing 1 - 2 of 2 FAQ).
Regarding recommended dilutions the manual contains: "Immunohistochemistry-Paraffin, Western Blot 1-3 ug/ml" Does it mean 1-3 ug/ml is recommended for both applications or there is simply no recommended dilution IHC-P?
For our product NB100-56404, we did not have a dilution recommendation listed for IHC on our datasheet. I am sorry for any confusion. This application was added to our product based on a publication that actually uses this product at 1:200 in paraformaldehyde fixed frozen tissue samples: NCBI site.
Which isoform of CXCR3 is this antibody specific for?
NB100-56404 can only detect human CXCR3 isoforms 1 and 2 in WB and IHC-Fr, the validated applications.