CXCL9/MIG Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CXCL9/MIG Antibody [NBP1-31155] - A431 cells. Green: MIG protein stained by MIG antibody diluted at 1:1000. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: CXCL9/MIG Antibody [NBP1-31155] - Human lung cancer. MIG stained by MIG antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: CXCL9/MIG Antibody [NBP1-31155] - Lung adenocarcinoma; antibody dilution 1:250. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min.

Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL9/MIG antibody (NBP1-31155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CXCL9/MIG Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CXCL9/MIG Antibody - BSA Free (NBP1-31155) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-CXCL9/MIG Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CXCL9/MIG. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCL9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 14 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CXCL9/MIG Antibody - BSA Free

• chemokine (C-X-C motif) ligand 9
• CMK
• crg-10
• C-X-C motif chemokine 9
• CXCL9
• Gamma-interferon-induced monokine
• Humig
• MIG
• MIGSmall-inducible cytokine B9
• monokine induced by gamma interferon
• SCYB9Monokine induced by interferon-gamma

Background

The function of this gene has not been specifically defined; however, it is thought to be involved in T cell trafficking. This gene has been localized to 4q21 with INP10, which is also a member of the chemokine family of cytokines. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CXCL9/MIG Antibody (NBP1-31155)(2)

Publications using NBP1-31155

• Triulzi T, Forte L, Regondi V et al. HER2 signaling regulates the tumor immune microenvironment and trastuzumab efficacy OncoImmunology 2018-09-21 [PMID: 30546951] (IF/IHC, Human)
• Nagy E, Lei Y, Martinez-Martinez E et al. Interferon-gamma Released by Activated CD8(+) T Lymphocytes Impairs the Calcium Resorption Potential of Osteoclasts in Calcified Human Aortic Valves. Am. J. Pathol. 2017-04-18 [PMID: 28431214] (IF/IHC, Human)

Review for CXCL9/MIG Antibody (NBP1-31155) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 10/28/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human testis
• Species: Human
• Lot: 39848

Bioinformatics

• Gene Symbol: CXCL9
• Entrez:
  • 4283(Human)
• Uniprot:
  • Q07325()