Mouse CXCL8/IL-8 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Mouse CXCL8/IL-8 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The IL-8 Prom/LUCPorter™ reporter cell line can be used for screening of agonists, antagonists or signaling inhibitors related with IL-8 induction as well as for studying the IL-8 related signaling pathways. Induction of the IL-8 promoter by various Toll-like receptor ligands is shown in Figure 1. In the test, all Toll-like receptor ligands except TLR5 ligand (Refer to NBP2-26261) significantly mediated activation of the IL-8 promoter which was quantified by luciferase activity (Figure 1).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The IL-8 Prom/LUCPorter™ reporter cell line is a stably transfected RAW 264.7 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the IL-8 promoter.
• Target Species: Mouse
• Specificity: IL-8 Prom/LUCPorter™
• Selection Agent: Puromycin
• Growth Properties: Adherent Morphology: Macrophage
• RCL Type: Luciferase - (LUCPorter™)
• Host: RAW264.7
• Gene: CXCL8

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Agonist:
  • Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297
  • CpG oligodeoxynucleotides Mouse NBP2-26235
  • MALP-2, TLR6 and TLR2 ligand NBP2-26219
  • Flagellin, TLR5 Ligand NBP2-25289
  • Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand NBP2-26231
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-25287
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the IL-8 reporter cell line.
- Wash hands after handling the IL-8 reporter cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with the cell line.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Mouse CXCL8/IL-8 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• 3-10C
• AMCF-I
• C-X-C motif chemokine 8
• CXCL8
• CXCL8SCYB8
• Emoctakin
• GCP1
• GCP-1TSG-1
• IL8
• IL-8
• interleukin 8
• K60
• LAI
• LECT
• LUCT
• LYNAP
• MDNCF
• MDNCFb-ENAP
• member 8
• MONAP
• MONAPGCP1
• NAF
• NAP1
• NAP-1NAP1
• NCF
• Neutrophil-activating protein 1
• Protein 3-10C
• T cell chemotactic factor
• T-cell chemotactic factor
• TCF
• TSG1

Background

IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Publications for CXCL8/IL-8 Reporter Cell Line (NBP2-26291)(1)

Publication using NBP2-26291

• Feng D, Sangster-Guity N, Stone R et al. Differential requirement of histone acetylase and deacetylase activities for IRF5-mediated proinflammatory cytokine expression. J Immunol. 2010-11-15 [PMID: 20935208]

Bioinformatics

• Gene Symbol: CXCL8