CXCL8/IL-8 Antibodies: Novus Biologicals

MAB208

CXCL8/IL-8 Antibody (6217) [Unconjugated]

(1 Review)

100 μg

MAB208

HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

<b>Biological Strategies Validation. </b>Simple Western lane view shows human PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND₅₀ is typically 0.08-0.4 µg/mL." class="big_lightbox">

Recombinant Human IL-8/CXCL8 (Catalog # <a class=

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND₅₀ is typically 0.08-0.4 µg/mL." class="big_thumb" />

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8. e Immunoblot analysis confirming reversal of mitotic apoptosis following exogenous reconstitution of IL-8 in CXCL8-depleted cells. Experiment was performed as in a, with addition of exogenous IL-8 for the last 48 h. Cells were treated with nocodazole for 8 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8. f Immunoblot analysis detecting induction of mitotic apoptosis in response to WT1, CXCL8, or WT1 and CXCL8 knockdown compared to control knockdown in U2OS cells that were arrested in mitosis using nocodazole (8 h). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

CDK1/CDC14B-dependent phosphorylation of USP9X at serine 2563 promotes mitotic survival via WT1 and IL-8.a. i Immunoblot analysis revealing decreased mitotic apoptosis after CDC14B knockdown in USP9XWT but not in USP9XMut U2OS cells. Before lysis cells were transfected with control or CDC14B-directed siRNA, arrested in mitosis, and collected for analysis by western blot. Throughout this figure, mean and standard deviations as error bars are displayed. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32152317), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cytokines regulated by RanBP1 in lung cancer. (A) Identification of secreted factors regulated by RanBP1 using cytokine arrays. A549 cells are used, and the expression of RanBP1 is suppressed using si-RNA. (B) Comparison of the gene expression of cytokines regulated by RanBP1. (C) Analysis of cytokines regulated by RanBP1 using WB. (D) Comparison of the sphere formation ability after each neutralizing antibody treatment. The p value is less than 0.0001 versus the IgG Ab value. (E) Comparison of migration and invasion ability of cells after each neutralizing antibody treatment. Experiments performed in triplicate. (F) CSC marker protein analysis in A549 after treatment with IL-18 neutralizing antibody. (G) Confirmation of the expression levels of EMT marker proteins after treatment with the IL-18 neutralizing antibody. (H) Expression analysis of RanBP1 regulated by IL-18 using neutralizing antibodies. Error bars represent mean ± SD. Triplicate samples. * p < 0.05, ** p < 0.001, versus control. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37047826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Interaction between osteosarcoma and macrophages increased IL-8 production. A Cytokine arrays of 143B-Luc cells, human macrophages, and co-culture CM. B IL-8 production during the co-culture of OS cells (143B-Luc, SJSA-1) and macrophages (THP-1 Mφ, HMDMs). C IL-8 expression in macrophages stimulated with OS-CM. D IL-8 expression in OS cells stimulated with macrophage CM. E UMAP plot of OS lung metastases. F UMAP plot of Iba1, CD163, and IL-8 expression in myeloid cell clusters. G Violin plot depicting IL-8 expression in Iba1+/− or CD163+/− myeloid cell clusters. CM conditioned medium, HMDMs human monocyte-derived macrophages, OS osteosarcoma, THP-1 Mφ THP-1-derived macrophage. Data are presented as mean ± standard deviation. **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on proliferation, migration, and invasion of osteosarcoma cells.A Cell viability assay for OS cells treated with or without recombinant IL-8 (rIL-8, 10 ng/mL) using Cell Counting Kit-8. B, C Scratch and invasion assays for OS cells treated with or without co-culture CM. The scratch assay was performed for 12 h, and the invasion assay was conducted for 24 h. Scale bars represent 500 μm. D Cell viability assay for OS cells treated with or without co-culture CM and with or without anti-IL-8 antibodies (IL-8 Abs, 1 µg/mL) using Cell Counting Kit-8. E, F Scratch and invasion assays for OS cells treated with or without co-culture CM and with or without IL-8 Abs (100 ng/mL). The scratch assay was performed for 12 h, and the invasion assay was conducted for 24 h. Scale bars represent 500 μm. CM: conditioned medium, OS: osteosarcoma, ns: not significant. Data are presented as mean ± standard deviation. *p < 0.05 and **p < 0.01. All data were obtained from at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on lung metastasis. A Schematic showing the experimental design for OS tail vein injection. 143B-Luc cells were treated with DMEM or co-culture CM for 12 h prior to tail vein injection; 143B-Luc cells (1 × 106/mouse) were injected into the tail vein of 6–7 week-old BALB/c nu/nu mice. B IVIS imaging and quantification of lung metastasis 14 days after the injection of tumors pretreated with or without co-culture CM. C Hematoxylin and eosin staining of lung sections with or without co-culture CM and quantification of lung colonies. Scale bars represent 1 mm and 500 µm. D Immunohistochemistry of Ki-67 positive cells in lung colonies with or without co-culture CM and quantification of the Ki-67 labeling index. Two colonies per sample were randomly selected, and quantification was performed for 10 colonies. Ki-67-positive cells in the colonies were counted and shown as the Ki-67 labeling index. Scale bars represent 100 µm. E Schematic showing the experimental design for OS tail vein injection using anti-IL-8 antibody. F IVIS imaging and quantification of lung metastasis 14 days after the injection of tumors pretreated with co-culture CM with or without IL-8 antibodies. G Hematoxylin and eosin staining of lung sections with co-culture CM with or without IL-8 Abs and quantification of lung colonies. Scale bars represent 1 mm and 500 µm. H Immunohistochemistry of Ki-67-positive cells in lung colonies with co-culture CM with or without IL-8 Abs and quantification of Ki-67 labeling index. Scale bars represent 100 µm. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, IVIS, in vivo imaging system, OS osteosarcoma. Data are presented as mean ± standard deviation; *p < 0.05, **p < 0.01. Each group contained five animals. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of IL-8 in co-culture CM on primary growth. A Schematic showing the experimental design for subcutaneous transplantation of OS cells. 143B-Luc cells (2 × 106/mouse) were subcutaneously transplanted in 6–7-week-old BALB/c nu/nu mice, and DMEM or co-culture CM was injected into the para-tumor thrice per week. B Tumor volume with or without co-culture CM measured thrice per week after tumor transplantation. C, D Tumor weight and image of the excised tumor with or without co-cultured CM 14 days after tumor transplantation. E, F Immunohistochemistry and quantification of Ki-67 and phospho-FAK in tumor sections with or without co-culture CM 14 days after tumor transplantation. Two fields of view per sample were randomly selected, and quantification was performed in 10 fields. E Ki-67-positive cells in the field were counted and indicated as Ki-67 labeling index. Scale bars represent 50 μm. G Schematic showing the experimental design for subcutaneous OS transplantation using anti-IL-8 antibodies (IL-8 Abs). H Tumor volume after pretreatment with co-culture CM with or without IL-8 Abs (10 µg/mouse) measured thrice per week after tumor transplantation. I and J Weight and image of the excised tumor pretreated with co-culture CM with or without IL-8 Abs 14 days after tumor transplantation. K, L Immunohistochemistry and quantification of Ki-67 and phospho-FAK in tumor sections with co-culture CM with or without IL-8 Abs 14 days after tumor transplantation. Scale bars represent 50 µm. CM conditioned medium, DMEM Dulbecco’s modified Eagle’s medium, FAK focal adhesion kinase, OS osteosarcoma. Data are presented as mean ± standard deviation; *p < 0.05, **p < 0.01. Each group contained five animals. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41419-024-06487-y), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC208U-100UG
Alexa Fluor 405	IC208V-100UG
Alexa Fluor 488	IC208G-100UG
Alexa Fluor 532	FAB208X
Alexa Fluor 594	IC208T-100UG
Alexa Fluor 647	IC208R-100UG
Alexa Fluor 700	IC208N-100UG
Alexa Fluor 750	IC208S-100UG
Alexa Fluor Plus 405	FAB208AFP405-100UG
Alexa Fluor Plus 488	FAB208AFP488-100UG
Alexa Fluor Plus 555	FAB208AFP555-100UG
Alexa Fluor Plus 594	FAB208AFP594-100UG
Alexa Fluor Plus 647	FAB208AFP647-100UG
Alexa Fluor Plus 680	FAB208AFP680-100UG
Allophycocyanin	FAB208A
Biotin	FAB208B
CoraFluor 1	FAB208CL1
DyLight 350	FAB208D
DyLight 405	FAB208E
DyLight 488	FAB208K
DyLight 550	FAB208L
DyLight 594	FAB208M
DyLight 650	FAB208W
DyLight 680	FAB208Y
DyLight 755	FAB208Z
Fluorescein	IC208F
HRP	FAB208H
Janelia Fluor 525	FAB208JF525
Janelia Fluor 549	FAB208I
Janelia Fluor 646	FAB208J
Janelia Fluor 669	FAB208JF669
PerCP	FAB208C
Phycoerythrin	IC208P
mFluor Violet 450 SE	FAB208MFV450
mFluor Violet 500 SE	FAB208MFV500
mFluor Violet 610 SE	FAB208MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC208F
Carrier Free	IC208P
Carrier Free	MAB208-SP
Carrier Free	MAB208-500
Carrier Free	IC208V-100UG
Carrier Free	IC208U-100UG
Carrier Free	IC208T-100UG
Carrier Free	IC208S-100UG
Carrier Free	IC208R-100UG
Carrier Free	IC208N-100UG
Carrier Free	IC208G-100UG

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AF-208-NA

CXCL8/IL-8 Antibody [Unconjugated]

(4 Reviews)

(26 Publications)

100 μg

AF-208-NA

HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates and conditioned media of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates and conditioned media of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hrs and 10ug/mL LPS for 3 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox">

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. " class="big_lightbox">

IL-8/CXCL8 was detected in immersion fixed paraffin-embedded sections of human tonsil (surgically removed due to severe EBV-induced mononucleosis) using Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑208‑NA) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for <a class=

Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. " class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-208-NAU-100UG
Alexa Fluor 405	AF-208-NAV-100UG
Alexa Fluor 488	AF-208-NAG-100UG
Alexa Fluor 532	AF-208-NAX-100UG
Alexa Fluor 594	AF-208-NAT-100UG
Alexa Fluor 647	AF-208-NAR-100UG
Alexa Fluor 700	AF-208-NAN-100UG
Alexa Fluor 750	AF-208-NAS-100UG
Alexa Fluor Plus 405	AF-208-NAAFP405
Alexa Fluor Plus 488	AF-208-NAAFP488
Alexa Fluor Plus 555	AF-208-NAAFP555
Alexa Fluor Plus 594	AF-208-NAAFP594
Alexa Fluor Plus 647	AF-208-NAAFP647
Alexa Fluor Plus 680	AF-208-NAAFP680
Allophycocyanin	AF-208-NAA
Biotin	BAF208
CoraFluor 1	AF-208-NACL1
DyLight 350	AF-208-NAD
DyLight 405	AF-208-NAE
DyLight 488	AF-208-NAK
DyLight 550	AF-208-NAL
DyLight 594	AF-208-NAM
DyLight 650	AF-208-NAW
DyLight 680	AF-208-NAY
DyLight 755	AF-208-NAZ
FITC	AF-208-NAF
HRP	AF-208-NAH
Janelia Fluor 525	AF-208-NAJF525
Janelia Fluor 549	AF-208-NAI
Janelia Fluor 646	AF-208-NAJ
Janelia Fluor 669	AF-208-NAJF669
PE	AF-208-NAP
PerCP	AF-208-NAC
mFluor Violet 450 SE	AF-208-NAMFV450
mFluor Violet 500 SE	AF-208-NAMFV500
mFluor Violet 610 SE	AF-208-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-208-SP

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NBP2-33819

CXCL8/IL-8 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP2-33819

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human rectum shows moderate positivity in plasma.

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human fallopian tube shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human lymph node shows weak to moderate cytoplasmic positivity in a subset of non-germinal center cells.

Immunohistochemistry-Paraffin: CXCL8/IL-8 Antibody [NBP2-33819] - Staining of human placenta shows moderate positivity in plasma.

(A, B) mRNA expression changes of acne inflammatory markers in sqRT-PCR. The reduction of inflammatory markers was large in the PDT group in which FDT gel and LED irradiation were applied together, in particular, it was greatest in the group in which LED irradiation was applied for 15 minutes. (C, D) Protein expression changes of acne inflammatory markers as indicated by Western blot analysis. The reduction of inflammatory markers was large in the PDT group in which FDT gel and LED irradiation were applied together; in particular, the greatest change was in the group in which LED irradiation was applied for 15 minutes. (B, D) * indicates p<0.05 statistical significance.IL: interleukin, TNF: tumor necrosis factor, TLR: Toll-like receptor, MMP: matrix metalloproteinase. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39623608), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-33819-25ul

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AB-208-NA

CXCL8/IL-8 Antibody [Unconjugated]

(1 Review)

(13 Publications)

1 mg

AB-208-NA

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox">

208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-8/CXCL8 Polyclonal Antibody (Catalog # AB-208-NA). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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MAB5351

CXCL8/IL-8 Antibody (105105) [Unconjugated]

(9 Publications)

100 μg

MAB5351

535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND₅₀ is typically 1-6 µg/mL." class="big_lightbox">

Recombinant Porcine IL-8/CXCL8 (Catalog # <a class=

535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND₅₀ is typically 1-6 µg/mL." alt="Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Porcine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB5351). The ND₅₀ is typically 1-6 µg/mL." class="big_thumb" />

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB5351U-100UG
Alexa Fluor 405	FAB5351V-100UG
Alexa Fluor 488	FAB5351G-100UG
Alexa Fluor 532	FAB5351X-100UG
Alexa Fluor 594	FAB5351T-100UG
Alexa Fluor 647	FAB5351R-100UG
Alexa Fluor 700	FAB5351N-100UG
Alexa Fluor 750	FAB5351S-100UG
Alexa Fluor Plus 405	FAB5351AFP405-100UG
Alexa Fluor Plus 488	FAB5351AFP488-100UG
Alexa Fluor Plus 555	FAB5351AFP555-100UG
Alexa Fluor Plus 594	FAB5351AFP594-100UG
Alexa Fluor Plus 647	FAB5351AFP647-100UG
Alexa Fluor Plus 680	FAB5351AFP680-100UG
Allophycocyanin	FAB5351A
Biotin	FAB5351B
CoraFluor 1	FAB5351CL1
DyLight 350	FAB5351D
DyLight 405	FAB5351E
DyLight 488	FAB5351K
DyLight 550	FAB5351L
DyLight 594	FAB5351M
DyLight 650	FAB5351W
DyLight 680	FAB5351Y
DyLight 755	FAB5351Z
FITC	FAB5351F
HRP	FAB5351H
Janelia Fluor 525	FAB5351JF525
Janelia Fluor 549	FAB5351I
Janelia Fluor 646	FAB5351J
Janelia Fluor 669	FAB5351JF669
PE	FAB5351P
PerCP	FAB5351C
mFluor Violet 450 SE	FAB5351MFV450
mFluor Violet 500 SE	FAB5351MFV500
mFluor Violet 610 SE	FAB5351MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB5351-SP
Carrier Free	MAB5351-500

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AF535

CXCL8/IL-8 Antibody [Unconjugated]

(4 Publications)

100 μg

AF535

535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND₅₀ is typically 1.5-7.5 µg/mL." class="big_lightbox">

535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND₅₀ is typically 1.5-7.5 µg/mL." alt="Recombinant Porcine IL-8/CXCL8 (Catalog # 535-IN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Porcine IL-8/CXCL8 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF535). The ND₅₀ is typically 1.5-7.5 µg/mL." class="big_thumb" />

Inflammatory response in PAMs induced by PRRSV 5′UTR RNA and LPS. A, B PAMs were transfected with different doses of 5′UTR RNA (1, 2, and 4 μg/well) along with 1 μg/mL LPS. qRT-PCR and Western blot results showed that compared with PAMs in other groups, PAMs in the 4 μg 5′UTR RNA and 1 μg/mL LPS co-stimulation group produced higher levels of IL-1 beta (p < 0.05). C After co-stimulation, the relative expression level of IL-1 beta mRNA obtained was similar at the 12 and 24 h time-points. D 5′UTR RNA and LPS co-stimulation induced IL-1 beta expression in cells and supernatants. E, F 5′UTR RNA and LPS co-stimulation induced increased levels of IL-6, IL-8 and TNF-alpha mRNA and protein. Expression was normalized to that of GAPDH. Different letters (a, b, c, d, and e) on data indicate significant differences between groups (p < 0.05). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31300043), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Po

Species Glossary

Reactivity: Po

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF535U-100UG
Alexa Fluor 405	AF535V-100UG
Alexa Fluor 488	AF535G-100UG
Alexa Fluor 532	AF535X-100UG
Alexa Fluor 594	AF535T-100UG
Alexa Fluor 647	AF535R-100UG
Alexa Fluor 700	AF535N-100UG
Alexa Fluor 750	AF535S-100UG
Alexa Fluor Plus 405	AF535AFP405-100UG
Alexa Fluor Plus 488	AF535AFP488-100UG
Alexa Fluor Plus 555	AF535AFP555-100UG
Alexa Fluor Plus 594	AF535AFP594-100UG
Alexa Fluor Plus 647	AF535AFP647-100UG
Alexa Fluor Plus 680	AF535AFP680-100UG
Allophycocyanin	AF535A
Biotin	BAF535
CoraFluor 1	AF535CL1
DyLight 350	AF535D
DyLight 405	AF535E
DyLight 488	AF535K
DyLight 550	AF535L
DyLight 594	AF535M
DyLight 650	AF535W
DyLight 680	AF535Y
DyLight 755	AF535Z
FITC	AF535F
HRP	AF535H
Janelia Fluor 525	AF535JF525
Janelia Fluor 549	AF535I
Janelia Fluor 646	AF535J
Janelia Fluor 669	AF535JF669
PE	AF535P
PerCP	AF535C
mFluor Violet 450 SE	AF535MFV450
mFluor Violet 500 SE	AF535MFV500
mFluor Violet 610 SE	AF535MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF535-SP

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NBP2-16958

CXCL8/IL-8 Antibody - BSA Free

(5 Publications)

0.1 ml

NBP2-16958

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8/IL-8 antibody (NBP2-16958) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: CXCL8/IL-8 Antibody [NBP2-16958] - Human leukocyte (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with CXCL8/IL-8 antibody (NBP2-16958) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, RM

Species Glossary

Reactivity: Hu, RM

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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MAB16081

CXCL8/IL-8 Antibody (258901) [Unconjugated]

(2 Publications)

500 μg

MAB16081

1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CXCL8/IL-8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB16081). The ND₅₀ is typically 3-12 µg/mL." class="big_lightbox">

Recombinant Canine CXCL8/IL-8 (Catalog # <a class=

1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CXCL8/IL-8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB16081). The ND₅₀ is typically 3-12 µg/mL." alt="Recombinant Canine CXCL8/IL-8 (Catalog # 1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine CXCL8/IL-8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine CXCL8/IL-8 Monoclonal Antibody (Catalog # MAB16081). The ND₅₀ is typically 3-12 µg/mL." class="big_thumb" />

Reactivity:

Ca

Species Glossary

Reactivity: Ca

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB16081U-100UG
Alexa Fluor 405	FAB16081V-100UG
Alexa Fluor 488	FAB16081G-100UG
Alexa Fluor 532	FAB16081X-100UG
Alexa Fluor 594	FAB16081T-100UG
Alexa Fluor 647	FAB16081R-100UG
Alexa Fluor 700	FAB16081N-100UG
Alexa Fluor 750	FAB16081S-100UG
Alexa Fluor Plus 405	FAB16081AFP405-100UG
Alexa Fluor Plus 488	FAB16081AFP488-100UG
Alexa Fluor Plus 555	FAB16081AFP555-100UG
Alexa Fluor Plus 594	FAB16081AFP594-100UG
Alexa Fluor Plus 647	FAB16081AFP647-100UG
Alexa Fluor Plus 680	FAB16081AFP680-100UG
Allophycocyanin	FAB16081A
Biotin	FAB16081B
CoraFluor 1	FAB16081CL1
DyLight 350	FAB16081D
DyLight 405	FAB16081E
DyLight 488	FAB16081K
DyLight 550	FAB16081L
DyLight 594	FAB16081M
DyLight 650	FAB16081W
DyLight 680	FAB16081Y
DyLight 755	FAB16081Z
FITC	FAB16081F
HRP	FAB16081H
Janelia Fluor 525	FAB16081JF525
Janelia Fluor 549	FAB16081I
Janelia Fluor 646	FAB16081J
Janelia Fluor 669	FAB16081JF669
PE	FAB16081P
PerCP	FAB16081C
mFluor Violet 450 SE	FAB16081MFV450
mFluor Violet 500 SE	FAB16081MFV500
mFluor Violet 610 SE	FAB16081MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB16081-SP

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AF1608

CXCL8/IL-8 Antibody [Unconjugated]

100 μg

AF1608

1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1608). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_lightbox">

Recombinant Canine IL-8/CXCL8 (Catalog # <a class=

1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1608). The ND₅₀ is typically 0.1-0.5 µg/mL." alt="Recombinant Canine IL-8/CXCL8 (Catalog # 1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1608). The ND₅₀ is typically 0.1-0.5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>IL-8/CXCL8 was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A using Goat Anti-Canine IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1608) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Ca

Species Glossary

Reactivity: Ca

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1608U-100UG
Alexa Fluor 405	AF1608V-100UG
Alexa Fluor 488	AF1608G-100UG
Alexa Fluor 532	AF1608X-100UG
Alexa Fluor 594	AF1608T-100UG
Alexa Fluor 647	AF1608R-100UG
Alexa Fluor 700	AF1608N-100UG
Alexa Fluor 750	AF1608S-100UG
Alexa Fluor Plus 405	AF1608AFP405-100UG
Alexa Fluor Plus 488	AF1608AFP488-100UG
Alexa Fluor Plus 555	AF1608AFP555-100UG
Alexa Fluor Plus 594	AF1608AFP594-100UG
Alexa Fluor Plus 647	AF1608AFP647-100UG
Alexa Fluor Plus 680	AF1608AFP680-100UG
Allophycocyanin	AF1608A
Biotin	BAF1608
CoraFluor 1	AF1608CL1
DyLight 350	AF1608D
DyLight 405	AF1608E
DyLight 488	AF1608K
DyLight 550	AF1608L
DyLight 594	AF1608M
DyLight 650	AF1608W
DyLight 680	AF1608Y
DyLight 755	AF1608Z
FITC	AF1608F
HRP	AF1608H
Janelia Fluor 525	AF1608JF525
Janelia Fluor 549	AF1608I
Janelia Fluor 646	AF1608J
Janelia Fluor 669	AF1608JF669
PE	AF1608P
PerCP	AF1608C
mFluor Violet 450 SE	AF1608MFV450
mFluor Violet 500 SE	AF1608MFV500
mFluor Violet 610 SE	AF1608MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1608-SP

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AF2277

CXCL8/IL-8 Antibody [Unconjugated]

100 μg

AF2277

2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox">

Recombinant Feline IL-8/CXCL8 (Catalog # <a class=

2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL-8/CXCL8 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Fe

Species Glossary

Reactivity: Fe

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2277U-100UG
Alexa Fluor 405	AF2277V-100UG
Alexa Fluor 488	AF2277G-100UG
Alexa Fluor 532	AF2277X-100UG
Alexa Fluor 594	AF2277T-100UG
Alexa Fluor 647	AF2277R-100UG
Alexa Fluor 700	AF2277N-100UG
Alexa Fluor 750	AF2277S-100UG
Alexa Fluor Plus 405	AF2277AFP405-100UG
Alexa Fluor Plus 488	AF2277AFP488-100UG
Alexa Fluor Plus 555	AF2277AFP555-100UG
Alexa Fluor Plus 594	AF2277AFP594-100UG
Alexa Fluor Plus 647	AF2277AFP647-100UG
Alexa Fluor Plus 680	AF2277AFP680-100UG
Allophycocyanin	AF2277A
Biotin	AF2277B
CoraFluor 1	AF2277CL1
DyLight 350	AF2277D
DyLight 405	AF2277E
DyLight 488	AF2277K
DyLight 550	AF2277L
DyLight 594	AF2277M
DyLight 650	AF2277W
DyLight 680	AF2277Y
DyLight 755	AF2277Z
FITC	AF2277F
HRP	AF2277H
Janelia Fluor 525	AF2277JF525
Janelia Fluor 549	AF2277I
Janelia Fluor 646	AF2277J
Janelia Fluor 669	AF2277JF669
PE	AF2277P
PerCP	AF2277C
mFluor Violet 450 SE	AF2277MFV450
mFluor Violet 500 SE	AF2277MFV500
mFluor Violet 610 SE	AF2277MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2277-SP

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MAB1608

CXCL8/IL-8 Antibody (258911) [Unconjugated]

100 μg

MAB1608

1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB1608). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_lightbox">

1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB1608). The ND₅₀ is typically 0.3-1.2 µg/mL." alt="Recombinant Canine IL-8/CXCL8 (Catalog # 1608-CL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Canine IL-8/CXCL8 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB1608). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

Reactivity:

Ca

Species Glossary

Reactivity: Ca

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1608U-100UG
Alexa Fluor 405	FAB1608V-100UG
Alexa Fluor 488	FAB1608G-100UG
Alexa Fluor 532	FAB1608X-100UG
Alexa Fluor 594	FAB1608T-100UG
Alexa Fluor 647	FAB1608R-100UG
Alexa Fluor 700	FAB1608N-100UG
Alexa Fluor 750	FAB1608S-100UG
Alexa Fluor Plus 405	FAB1608AFP405-100UG
Alexa Fluor Plus 488	FAB1608AFP488-100UG
Alexa Fluor Plus 555	FAB1608AFP555-100UG
Alexa Fluor Plus 594	FAB1608AFP594-100UG
Alexa Fluor Plus 647	FAB1608AFP647-100UG
Alexa Fluor Plus 680	FAB1608AFP680-100UG
Allophycocyanin	FAB1608A
Biotin	BAM16082
CoraFluor 1	FAB1608CL1
DyLight 350	FAB1608D
DyLight 405	FAB1608E
DyLight 488	FAB1608K
DyLight 550	FAB1608L
DyLight 594	FAB1608M
DyLight 650	FAB1608W
DyLight 680	FAB1608Y
DyLight 755	FAB1608Z
FITC	FAB1608F
HRP	FAB1608H
Janelia Fluor 525	FAB1608JF525
Janelia Fluor 549	FAB1608I
Janelia Fluor 646	FAB1608J
Janelia Fluor 669	FAB1608JF669
PE	FAB1608P
PerCP	FAB1608C
mFluor Violet 450 SE	FAB1608MFV450
mFluor Violet 500 SE	FAB1608MFV500
mFluor Violet 610 SE	FAB1608MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1608-SP

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MAB22772

CXCL8/IL-8 Antibody (344615) [Unconjugated]

500 μg

MAB22772

2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB22772). The ND₅₀ is typically 0.075-0.375 µg/mL." class="big_lightbox">

2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB22772). The ND₅₀ is typically 0.075-0.375 µg/mL." alt="Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB22772). The ND₅₀ is typically 0.075-0.375 µg/mL." class="big_thumb" />

Reactivity:

Fe

Species Glossary

Reactivity: Fe

Applications:

,

Applications: ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB22772U-100UG
Alexa Fluor 405	FAB22772V-100UG
Alexa Fluor 488	FAB22772G-100UG
Alexa Fluor 532	FAB22772X-100UG
Alexa Fluor 594	FAB22772T-100UG
Alexa Fluor 647	FAB22772R-100UG
Alexa Fluor 700	FAB22772N-100UG
Alexa Fluor 750	FAB22772S-100UG
Alexa Fluor Plus 405	FAB22772AFP405-100UG
Alexa Fluor Plus 488	FAB22772AFP488-100UG
Alexa Fluor Plus 555	FAB22772AFP555-100UG
Alexa Fluor Plus 594	FAB22772AFP594-100UG
Alexa Fluor Plus 647	FAB22772AFP647-100UG
Alexa Fluor Plus 680	FAB22772AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB22772-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CXCL8/IL-8 Antibodies

CXCL8/IL-8 Antibody (6217) [Unconjugated]

CXCL8/IL-8 Antibody [Unconjugated]

CXCL8/IL-8 Antibody - BSA Free

CXCL8/IL-8 Antibody [Unconjugated]

CXCL8/IL-8 Antibody (105105) [Unconjugated]

CXCL8/IL-8 Antibody [Unconjugated]

CXCL8/IL-8 Antibody - BSA Free

CXCL8/IL-8 Antibody (258901) [Unconjugated]

CXCL8/IL-8 Antibody [Unconjugated]

CXCL8/IL-8 Antibody [Unconjugated]

CXCL8/IL-8 Antibody (258911) [Unconjugated]

CXCL8/IL-8 Antibody (344615) [Unconjugated]