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HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically 0.08-0.4 µg/mL." class="big_lightbox" target="_blank">
208-IL) was serially diluted and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF017). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox" target="_blank">
HAF019). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox" target="_blank">
208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA). The ND50 is typically 0.1-0.5 µg/mL." class="big_lightbox" target="_blank">
208-IL) was serially diluted and captured by Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-208-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/images/antibody/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-1882021143847.jpg)
![Western Blot CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-208-na_human-il-8-cxcl8-affinity-purified-polyclonal-ab-western-blot-1882021144622.jpg)
![Sandwich ELISA CXCL8/IL-8 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/CXCL8-IL-8-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79438-img0001.jpg)
![N/A CXCL8/IL-8 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IL8_DY208_ELISA_2307.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CXCL8_IL8_D8050_ELISA_557.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CXCL8_IL8_HS800_ELISA_892.jpg)
![Bioactivity CXCL8/IL-8 [Unconjugated]](http://images.novusbio.com/fullsize/protein/CXCL8_IL-8_208-IL_57.jpg)
![Immunocytochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/images/antibody/CXCL8_MAB208_Immunocytochemistry_9339.jpg)
![Simple Western CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/antibody/mab208_human-il-8-cxcl8-mab-clone-6217-simple-western-1072020142347.jpg)
![Neutralization CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/images/antibody/CXCL8_MAB208_Block_Neutralize_9064.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-1212202581188.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-12122025829014.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-12122025821215.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab7155_mouse-ikk-beta-mab-clone-725818-western-blot-121220258481611.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-121220259231619.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-121220259221220.jpg)
![Western Blot CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-western-blot-12122025971810.jpg)
![Flow Cytometry CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-flow-cytometry-12122025931122.jpg)
![Immunohistochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-immunohistochemistry-121220259302813.jpg)
![Immunohistochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-immunohistochemistry-12122025930715.jpg)
![Immunohistochemistry CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-immunohistochemistry-121220259294522.jpg)
![Flow Cytometry CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/fullsize/mab208_human-il-8-cxcl8-mab-clone-6217-flow-cytometry-1212202593078.jpg)
![ELISA CXCL8/IL-8 Antibody (6217) [Unconjugated]](http://images.novusbio.com/images/mab208_human-il-8-cxcl8-mab-clone-6217-elisa-639201776107916141.jpg)
![Simple Western CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-208-na_human-il-8-cxcl8-affinity-purified-polyclonal-ab-simple-western-1072020121328.jpg)
![Simple Western CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-208-na_human-il-8-cxcl8-affinity-purified-polyclonal-ab-simple-western-107202012618.jpg)
![Neutralization CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CXCL8_AF-208-NA_Block_Neutralize_8646.jpg)
![Immunocytochemistry CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CXCL8_AF-208-NA_Immunocytochemistry_6460.jpg)
![Immunohistochemistry CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CXCL8_AF-208-NA_Immunohistochemistry_7081.jpg)
![ELISA CXCL8/IL-8 Antibody [Unconjugated]](http://images.novusbio.com/images/af-208-na_human-il-8-cxcl8-affinity-purified-polyclonal-ab-elisa-639201852922655258.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CXCL8_IL8_D8050_ELISA_558.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/CXCL8_D8000C_ELISA_378.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-44202415425068.jpg)
![ELISA CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-2282024179564.jpg)
![ELISA CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-22820241795613.jpg)
![ELISA CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-2282024179569.jpg)
![ELISA CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/d8000c_human-il-8-cxcl8-quantikine-elisa-kit-22820241723281.jpg)
![N/A CXCL8/IL-8 [HRP]](http://images.novusbio.com/fullsize/elisa/CXCL8_HS800_ELISA_216.jpg)
![SDS-Page CXCL8/IL-8 [Unconjugated]](http://images.novusbio.com/fullsize/protein/CXCL8_IL-8_208-IL_56.jpg)
