CXCL7/NAP-2 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL7/NAP-2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CXCL7/NAP-2 (R&D Systems, Catalog # 393-NP)
  Ala59-Asp128
  Accession # P02775
• Specificity: Detects human CXCL7/NAP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) ENA-70, rhENA-74, rhENA-78, rhGCP-2, rhGRO alpha , rhGRO beta , rhGRO gamma , rhIL-8, rhIP-10, rhMIG, rhSDF‑1 alpha , and rhSDF‑1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPBP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture:Human CXCL7/NAP-2 Antibody (Catalog # MAB393)
  ELISA Detection: Human CXCL7/NAP-2 Biotinylated Antibody (Catalog # BAF393)
  Standard: Recombinant Human CXCL7/NAP-2 (Catalog # 393-NP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP‑III) and beta -thrombogulin ( beta ‑TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

1. Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
2. Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPBP