CXCL7/NAP-2 Antibody [Unconjugated]

Simple Western lane view shows lysates of human blood platelets, loaded at 0.2 mg/mL. A specific band was detected for CXCL7/NAP‑2 at approximately 7 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CXCL7/NAP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF393) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCL7/NAP-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL7/NAP-2
  Ala59-Asp128
  Accession # P02775
• Specificity: Detects human CXCL7/NAP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 10% cross-reactivity with recombinant human (rh) GRO alpha , rhIL-8, rhCINC-2 alpha , and rhGRO beta is observed, and 5% cross-reactivity with recombinant rat (rr) CINC-1, rrCINC-2 beta , rhGCP-2, rmKC, rhMIG, and rhGRO beta . is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPBP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL7/NAP-2 Antibody [Unconjugated]

• Beta-TG
• connective tissue-activating peptide III
• CTAP III
• CTAP3CTAP-III
• CTAPIII
• CXC chemokine ligand 7
• C-X-C motif chemokine 7
• CXCL7
• CXCL7b-TG1
• LA-PF4
• LDGFTC1
• Leukocyte-derived growth factor
• low-affinity platelet factor IV
• Macrophage-derived growth factor
• MDGFTC2
• NAP2
• NAP-2
• NAP-2-L1
• neutrophil-activating peptide 2
• neutrophil-activating peptide-2
• PBPTHBGB
• platelet basic protein
• PPBP
• pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• SCAR10
• SCYB7beta-thromboglobulin
• small inducible cytokine B7
• small inducible cytokine subfamily B, member 7
• Small-inducible cytokine B7
• TGB
• TGB1B-TG1
• THBGB1
• thrombocidin 1
• thrombocidin 2
• thromboglobulin, beta-1

Background

Neutrophil Activating Peptide 2 (NAP-2), Connective Tissue Activating Protein III (CTAP‑III) and beta -thrombogulin ( beta ‑TG), are proteolytically processed carboxyl-terminal fragments of platelet basic protein (PBP) which is found in the alpha-granules of human platelets. NAP-2 is a member of the CXC chemokines. Similar to other ELR domain containing CXC chemokines such as IL-8 and the GRO proteins, NAP-2 has been shown to bind CXCR2 and to chemoattract and activate neutrophils. Although CTAP-III, beta -TG and PBP represent amino-terminal extended variants of NAP-2 and possess the same CXC chemokine domains, these proteins do not exhibit NAP-2 activity. It has been shown that the additional amino-terminal residues of CTAP-III masks the critical ELR receptor binding domain that is exposed on NAP-2 and may account for lack of NAP-2 activity.

1. Schall, T. (1994) The Cytokine Handbook, 2nd edition, A. Thomson, ed. Academic Press, New York, p. 419.
2. Malkowski, M.G. et al. (1997) J. Mol. Biol. 266:367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL7/NAP-2 Antibody (AF393)(1)

Publication using AF393

• GK Annarapu, D Nolfi-Done, M Reynolds, Y Wang, L Kohut, B Zuckerbrau, S Shiva Heme stimulates platelet mitochondrial oxidant production to induce targeted granule secretion Redox Biology, 2021-12-05;48(0):102205. 2021-12-05 [PMID: 34891098] (Dot Blot, Human)

Bioinformatics

• Gene Symbol: PPBP
• Uniprot:
  • P02775()